BCR-ABL1 transcript levels increase in peripheral blood but not in granulocytes after physical exercise in patients with chronic myeloid leukemia

Abstract

In chronic myeloid leukemia (CML) treatment response is determined by measurements of BCR-AB1L transcripts in peripheral blood by quantitative real-time PCR (qRT-PCR) and a 2-5 fold increase is considered a warning sign. The BCR-ABL1 gene is mainly expressed in myeloid cells whereas quantification of BCR-ABL1 is performed on the nucleated cell fraction of peripheral blood. Hence, leukocyte composition of the nucleated cell fraction may affect the result of BCR-ABL1 quantification. The aim of this study was to investigate if changes in leukocyte composition of peripheral blood had any effect on BCR-ABL1 transcript levels in CML patients. Six CML patients in complete cytogenetic remission (CCgR) performed a maximal physical exercise test. Blood samples were collected before exercise, at maximal exhaustion and after exercise. A biphasic increase in leukocyte count was observed and the relative proportion of granulocytes in peripheral blood changed significantly after exercise compared with baseline (p < 0.001). The BCR-ABL1 transcript level increased significantly following exercise, in nucleated cell fraction of peripheral blood (p < 0.05) but not in isolated granulocytes. In the nucleated cell fraction, the mean BCR-ABL1 transcript level was 3.3-fold (range 0.7-6.8) higher 180 min after exercise compared with baseline (p < 0.01). In conclusion, physical exercise induced significant increases in BCR-ABL1 transcript levels concomitant with changes in leukocyte content of peripheral blood. We therefore suggest that variations in leukocyte composition of peripheral blood, causing pre-analytic variations that affect BCR-ABL1 quantification, have to be accounted for. Consequently, small variations in BCR-ABL1 transcript levels should be interpreted cautiously in CML patients in CCgR.

Natural killer cell mobilization and egress following acute exercise in men with prostate cancer

“…However, the immune response to exercise in cancer survivors has only been superficially examined (Khosravi et al., 2019; Kruijsen‐Jaarsma et al., 2013). Complete blood counts are the most common outcome (Galvao et al., 2008; Jonsson et al., 2011; Ladha, Courneya, Bell, Field, & Grundy, 2006; Shore & Shepard, 1999) with minimal work investigating changes in specific cell types (Evans et al., 2015; Zimmer et al., 2016). As the stress hormone response to acute exercise appears attenuated in prostate cancer (Hanson et al., 2018), this may diminish lymphocyte mobilization.…”

Implementação de um Protocolo Fisioterapêutico em Pacientes Hemato-Oncológicos

“…Em relação aos exames sanguíneos, não foram encontradas alterações significativas, ao contrário dos resultados de Jönsson et al 29 , que mostraram aumentos significativos nos leucócitos, granulócitos e linfócitos em coleta sanguínea. Contudo, os indivíduos foram submetidos a um teste de esforço em um cicloergômetro eletrônico à 60rpm e com aumento de carga de trabalho progressiva.…”

Revisiting the Concept of Stress in the Prognosis of Solid Tumors: A Role for Stress Granules Proteins?

“…SGs proteins regulator expression level can be used to estimate the capacity of cells composing a tissue to form SGs [ 22 , 57 , 58 , 76 ]. In line with this, we analyzed publicly available gene expression data of breast [ 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 ], colon [ 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 ], and pancreatic [ 122 , 123 , 124 , 125 , 126 ,…”