<i>C. elegans</i>Rab GTPase 2 is required for the degradation of apoptotic cells

Lu, Qun; Zhang, Yan; Hu, Tianjing; Guo, Pengfei; Li, Weida; Wang, Xiaochen

doi:10.1242/dev.016063

Cited by 51 publications

(65 citation statements)

References 45 publications

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“…Mutations in rab-14 and unc-108/RAB2 also caused defective residual body clearance, which was further enhanced in unc-108(n3263); rab-14(tm2095) double mutants (Fig. 3F,L), consistent with the redundant roles of UNC-108 and RAB-14 in regulating phagosome maturation Lu et al, 2008;Mangahas et al, 2008).…”

Section: Research Articlesupporting

confidence: 60%

Residual body removal during spermatogenesis in C. elegans requires genes that mediate cell corpse clearance

et al. 2012

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SUMMARYGeneration of spermatozoa involves segregation of most of the cytoplasm into residual bodies, which are detached from spermatids and eliminated in mammals. However, the molecular and cellular mechanism underlying the removal of residual bodies remains largely unknown. Here, we demonstrate that during C. elegans spermatogenesis residual bodies are engulfed and degraded by gonadal sheath cells, a process that uses the same set of genes underlying apoptotic cell removal. The two partially redundant engulfment pathways that clear cell corpses also mediate phagocytosis of residual bodies, possibly by recognizing the 'eat me' signal phosphatidylserine exposed on the surface. The residual body-containing phagosome undergoes a maturation process involving sequential steps including dynamic coating with PtdIns(3)P and association of RAB small GTPases. The genetic hierarchy of residual body removal in hermaphrodites is similar to that of cell corpse clearance, but male residual body removal involves a distinct hierarchy, with differential use of the engulfment genes. Efficient removal of residual bodies regulates the number of spermatids and effective transfer of spermatids during male matings. Our results indicate that a similar molecular mechanism is employed for the removal of residual bodies and apoptotic cell corpses in C. elegans.

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Section: Research Articlesupporting

confidence: 60%

Residual body removal during spermatogenesis in C. elegans requires genes that mediate cell corpse clearance

et al. 2012

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“…In addition, Rab2 has a role in phagosome maturation [42–45]. In C. elegans , mutations in Rab2/UNC-108 impair progression through the conventional endolysosomal pathway at both early and late stages [46,47]. However, the mechanism behind this function remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Drosophila Rab2 controls endosome-lysosome fusion and LAMP delivery to late endosomes

2018

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Rab2 is a conserved Rab GTPase with a well-established role in secretory pathway function and phagocytosis. Here we demonstrate that Drosophila Rab2 is recruited to late endosomal membranes, where it controls the fusion of LAMP-containing biosynthetic carriers and lysosomes to late endosomes. In contrast, the lysosomal GTPase Gie/Arl8 is only required for late endosome-lysosome fusion, but not for the delivery of LAMP to the endocytic pathway. We also find that Rab2 is required for the fusion of autophagosomes to the endolysosomal pathway, but not for the biogenesis of lysosome-related organelles. Surprisingly, Rab2 does not rely on HOPS-mediated vesicular fusion for recruitment to late endosomal membranes. Our work suggests that Drosophila Rab2 is a central regulator of the endolysosomal and macroautophagic/autophagic pathways by controlling the major heterotypic fusion processes at the late endosome.

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“…These apoptotic bodies are then phagocyted by surrounding phagocytes, thus preventing the release of the cytoplasmic content to the outside. Hence, the development of an inflammatory response and damage to the neighboring cells are avoided (49,50).…”

Section: Introductionmentioning

confidence: 99%

Cell Death Mechanisms Induced by Cytotoxic Lymphocytes

et al. 2009

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One of the functions of the immune system is to recognize and destroy abnormal or infected cells to maintain homeostasis. This is accomplished by cytotoxic lymphocytes. Cytotoxicity is a highly organized multifactor process. Here, we reviewed the apoptosis pathways induced by the two main cytotoxic lymphocyte subsets, natural killer (NK) cells and CD8 + T cells. In base to recent experimental evidence, we reviewed NK receptors involved in recognition of target-cell, as well as lytic molecules such as perforin, granzymes-A and -B, and granulysin. In addition, we reviewed the Fas-FasL intercellular linkage mediated pathway, and briefly the cross-linking of tumor necrosis factor (TNF) and TNF receptor pathway. We discussed three models of possible molecular interaction between lytic molecules from effector cytotoxic cells and target-cell membrane to induction of apoptosis. Cellular & Molecular Immunology. 2009;6(1):15-25.

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C. elegansRab GTPase 2 is required for the degradation of apoptotic cells

Cited by 51 publications

References 45 publications

Residual body removal during spermatogenesis in C. elegans requires genes that mediate cell corpse clearance

Residual body removal during spermatogenesis in C. elegans requires genes that mediate cell corpse clearance

Drosophila Rab2 controls endosome-lysosome fusion and LAMP delivery to late endosomes

Cell Death Mechanisms Induced by Cytotoxic Lymphocytes

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