<i>dnaJ</i>
            : a New Approach to Identify Species within the Genus Enterobacter

Alonso, Enrique Hernández; Barreault, Simon; Augusto, Luis; Jatteau, Pierre; Villet, Millie; Tissières, Pierre; Doucet‐Populaire, Florence; Bourgeois-Nicolaos, Nadège

doi:10.1128/spectrum.01242-21

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…These results support the hypothesis that E. bugandensis is an emerging pathogen in the NICU with a virulence potentially greater than other species of the genus Enterobacter ( 35 , 36 ). However, more studies implementing tools for precise species identification are needed in additional settings to better understand its epidemiology in the NICU ( 12 ).…”

Section: Discussionmentioning

confidence: 99%

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Contaminated Incubators: Source of a Multispecies Enterobacter Outbreak of Neonatal Sepsis

et al. 2022

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Section: Discussionmentioning

confidence: 99%

“…Over the last decades, the genus Enterobacter has emerged as an important nosocomial pathogen in NICUs ( 10 , 11 ). Today, more than 20 different species have been identified by molecular techniques ( 12 ). Enterobacter spp.…”

Section: Introductionmentioning

confidence: 99%

Contaminated Incubators: Source of a Multispecies Enterobacter Outbreak of Neonatal Sepsis

et al. 2022

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“…The hsp60ECCtool developed here provides a more evident identification of hsp60 clusters in the ECC than a direct comparison using BLASTn against the entire National Center for Biotechnology Information (NCBI) database. Like other recently proposed methods and markers, this updated approach should help the scientific community to rapidly sort and classify ECC members for epidemiological and screening purposes [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Emergence of a Novel Lineage and Wide Spread of a blaCTX-M-15/IncHI2/ST1 Plasmid among Nosocomial Enterobacter in Guadeloupe

et al. 2022

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Between April 2018 and August 2019, a total of 135 strains of Enterobacter cloacae complex (ECC) were randomly collected at the University Hospital Center of Guadeloupe to investigate the structure and diversity of the local bacterial population. These nosocomial isolates were initially identified genetically by the hsp60 typing method, which revealed the clinical relevance of E. xiangfangensis (n = 69). Overall, 57/94 of the third cephalosporin-resistant strains were characterized as extended-spectrum-β-lactamase (ESBL) producers, and their whole-genome was sequenced using Illumina technology to determine the clonal relatedness and diffusion of resistance genes. We found limited genetic diversity among sequence types (STs). ST114 (n = 13), ST1503 (n = 9), ST53 (n = 5) and ST113 (n = 4), which belong to three different Enterobacter species, were the most prevalent among the 57 ESBL producers. The blaCTXM-15 gene was the most prevalent ESBL determinant (56/57) and was in most cases associated with IncHI2/ST1 plasmid replicon carriage (36/57). To fully characterize this predominant blaCTXM-15/IncHI2/ST1 plasmid, four isolates from different lineages were also sequenced using Oxford Nanopore sequencing technology to generate long-reads. Hybrid sequence analyses confirmed the circulation of a well-conserved plasmid among ECC members. In addition, the novel ST1503 and its associated species (ECC taxon 4) were analyzed, in view of its high prevalence in nosocomial infections. These genetic observations confirmed the overall incidence of nosocomial ESBL Enterobacteriaceae infections acquired in this hospital during the study period, which was clearly higher in Guadeloupe (1.59/1000 hospitalization days) than in mainland France (0.52/1,000 hospitalization days). This project revealed issues and future challenges for the management and surveillance of nosocomial and multidrug-resistant Enterobacter in the Caribbean.

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Molecular characterization and in-depth genome analysis of Enterobacter sp. S-16

Kumari,

Sharma,

Shikha

et al. 2023

Funct Integr Genomics

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dnaJ : a New Approach to Identify Species within the Genus Enterobacter

Abstract: We propose a new approach for Enterobacter species identification based on the diversity of the gene encoding the heat shock protein DnaJ. This new tool can be easily implemented in clinical laboratories in addition to identification by MALDI-TOF.

Cited by 3 publications

References 29 publications

Contaminated Incubators: Source of a Multispecies Enterobacter Outbreak of Neonatal Sepsis

Contaminated Incubators: Source of a Multispecies Enterobacter Outbreak of Neonatal Sepsis

Emergence of a Novel Lineage and Wide Spread of a blaCTX-M-15/IncHI2/ST1 Plasmid among Nosocomial Enterobacter in Guadeloupe

Molecular characterization and in-depth genome analysis of Enterobacter sp. S-16

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