<i>dnm1</i> deletion blocks mitochondrial fragmentation in <i>Δfzo1</i> cells

Yang, Yanmei; Hu, Yadong; Zhang, Pan; Shang, Jinjie

doi:10.1002/yea.3524

Cited by 10 publications

(11 citation statements)

References 37 publications

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“…This result is similar to the one reported recently that mitochondria become tubular upon deletion of both dnm1 and fzo1 [14]. It was shown that mitochondrial morphology depends on the order of the deletion of dnm1 and fzo1 [14]. That is, mitochondrial morphology of dnm1 Δ fzo1 Δ cells is similar to the one of dnm1 Δ cells if dnm1 is deleted first, whereas mitochondrial morphology of dnm1 Δ fzo1 Δ cells is wild type‐like if fzo1 is deleted first [14].…”

Section: Resultssupporting

confidence: 92%

“…1 and 2). Our finding is consistent with the one reported recently that deletion of both dnm1 and fzo1 restores tubular mitochondrial morphology and restores cell growth on nonfermentable medium [14]. Therefore, it seems that mitochondrial morphology is a key factor in dictating mitochondrial functionality in fission yeast cells.…”

Section: Discussionsupporting

confidence: 93%

“…1A). This result is similar to the one reported recently that mitochondria become tubular upon deletion of both dnm1 and fzo1 [14]. It was shown that mitochondrial morphology depends on the order of the deletion of dnm1 and fzo1 [14].…”

Section: Fission Yeast Cells Lacking Both Dnm1 and Fzo1 Display Tubular Mitochondriasupporting

confidence: 92%

“…Therefore, fission yeast has proven to be an excellent model organism, complementing the budding yeast Saccharomyces cerevisiae , for mitochondria studies [13]. Recently, it was reported that the absence of Fzo1 in fission yeast causes fragmented mitochondria and reduced growth on nonfermentable medium but the absence of Dnm1 and Fzo1 restores tubular mitochondrial morphology and wild type‐like growth on nonfermentable medium [14]. These observations indicate that mitochondrial functionality, particularly mitochondrial respiration, is impaired in the absence of Fzo1; nonetheless, mitochondrial membrane potential and respiration in the mutants have not been examined directly.…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial fusion and fission are required for proper mitochondrial function and cell proliferation in fission yeast

et al. 2021

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Mitochondria form a branched tubular network in many types of cells, depending on a balance between mitochondrial fusion and fission. How mitochondrial fusion and fission are involved in regulating mitochondrial function and cell proliferation is not well understood. Here, we dissected the roles of mitochondrial fusion and fission in mitochondrial function and cell proliferation in fission yeast. We examined mitochondrial membrane potential by staining cells with DiOC 6 and assessed mitochondrial respiration by directly measuring oxygen consumption of cells with a dissolved oxygen respirometer. We found that defects in mitochondrial fission or fusion reduce mitochondrial membrane potential and compromise mitochondrial respiration while the absence of both mitochondrial fusion and fission restores wild type-like respiration, normal membrane potential, and tubular networks of mitochondria. Moreover, we found that the absence of either mitochondrial fission or fusion prolongs the cell cycle and that the absence of both mitochondrial fusion and fission significantly delays cell cycle progression after nitrogen replenishment. The prolonged/delayed cell cycle is likely due to the deregulation of Cdc2 activation. Hence, our work not only establishes an intimate link between mitochondrial morphology and function but also underscores the importance of mitochondrial dynamics in regulating the cell cycle.

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Section: Resultssupporting

confidence: 92%

Section: Discussionsupporting

confidence: 93%

Section: Fission Yeast Cells Lacking Both Dnm1 and Fzo1 Display Tubular Mitochondriasupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mitochondrial fusion and fission are required for proper mitochondrial function and cell proliferation in fission yeast

et al. 2021

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“…Taking these results into account, in order to improve the stability of endogenous 2µ-based expression vector in yeast, an essential gene could be introduced into the plasmid while knocking out the same essential gene in the genome to ensure the existence of engineered endogenous 2µ plasmid in yeast (Zeng et al, 2021). In the future, researchers could apply the CRISPR/Cas9 system to directly integrate metabolic pathways into the endogenous 2µ plasmid with an essential gene in vivo (Dean-Johnson and Henry, 1989;Zheng et al, 1993;Wang et al, 2020;Yang et al, 2021). In summary, our endogenous 2µ-based expression vector p2µM has improved stability than the commonly used YEp pRS423, so it could be applied in S. cerevisiae for genetic manipulations.…”

Section: Discussionmentioning

confidence: 99%

Harnessing the Endogenous 2μ Plasmid of Saccharomyces cerevisiae for Pathway Construction

et al. 2021

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pRS episomal plasmids are widely used in Saccharomyces cerevisiae, owing to their easy genetic manipulations and high plasmid copy numbers (PCNs). Nevertheless, their broader application is hampered by the instability of the pRS plasmids. In this study, we designed an episomal plasmid based on the endogenous 2μ plasmid with both improved stability and increased PCN, naming it p2μM, a 2μ-modified plasmid. In the p2μM plasmid, an insertion site between the REP1 promoter and RAF1 promoter was identified, where the replication (ori) of Escherichia coli and a selection marker gene of S. cerevisiae were inserted. As a proof of concept, the tyrosol biosynthetic pathway was constructed in the p2μM plasmid and in a pRS plasmid (pRS423). As a result, the p2μM plasmid presented lower plasmid loss rate than that of pRS423. Furthermore, higher tyrosol titers were achieved in S. cerevisiae harboring p2μM plasmid carrying the tyrosol pathway-related genes. Our study provided an improved genetic manipulation tool in S. cerevisiae for metabolic engineering applications, which may be widely applied for valuable product biosynthesis in yeast.

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Low‐Molecular Weight Compounds that Extend the Chronological Lifespan of Yeasts, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

Ohtsuka,

Shimasaki,

Aiba

2024

Advanced Biology

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Yeast is an excellent model organism for research for regulating aging and lifespan, and the studies have made many contributions to date, including identifying various factors and signaling pathways related to aging and lifespan. More than 20 years have passed since molecular biological perspectives are adopted in this research field, and intracellular factors and signal pathways that control aging and lifespan have evolutionarily conserved from yeast to mammals. Furthermore, these findings have been applied to control the aging and lifespan of various model organisms by adjustment of the nutritional environment, genetic manipulation, and drug treatment using low‐molecular weight compounds. Among these, drug treatment is easier than the other methods, and research into drugs that regulate aging and lifespan is consequently expected to become more active. Chronological lifespan, a definition of yeast lifespan, refers to the survival period of a cell population under nondividing conditions. Herein, low‐molecular weight compounds are summarized that extend the chronological lifespan of Saccharomyces cerevisiae and Schizosaccharomyces pombe, along with their intracellular functions. The low‐molecular weight compounds are also discussed that extend the lifespan of other model organisms. Compounds that have so far only been studied in yeast may soon extend lifespan in other organisms.

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dnm1 deletion blocks mitochondrial fragmentation in Δfzo1 cells

Cited by 10 publications

References 37 publications

Mitochondrial fusion and fission are required for proper mitochondrial function and cell proliferation in fission yeast

Mitochondrial fusion and fission are required for proper mitochondrial function and cell proliferation in fission yeast

Harnessing the Endogenous 2μ Plasmid of Saccharomyces cerevisiae for Pathway Construction

Low‐Molecular Weight Compounds that Extend the Chronological Lifespan of Yeasts, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

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