<i>Drosophila</i> β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules

Myachina, Faina; Bosshardt, Fritz; Bischof, Johannes; Kirschmann, Moritz; Lehner, Christian F.

doi:10.1242/dev.156109

Cited by 25 publications

(16 citation statements)

References 78 publications

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“…However, more detailed species-specific analysis will be required to confirm this observation. Along these lines, a recent observation revealed that the Drosophila btubulin97EF is upregulated at low temperatures and contribute to stabilize microtubules (26). This example demonstrates that differential regulation of intracellular components is necessary for acclimation to environmental changes.…”

Section: Discussionmentioning

confidence: 86%

Temporal development of Drosophila embryos is highly robust across a wide temperature range

Chong

Amourda

Saunders

2018

Preprint

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Development is a precisely coordinated process in both space and time. Spatial precision has been quantified in a number of developmental systems, and, for example, such data has contributed significantly to our understanding of morphogen gradient interpretation. However, comparatively little quantitative analysis has been performed on timing and temporal coordination during development. Here, we use Drosophila to explore the temporal robustness of embryonic development within physiologically normal temperatures. We find that development is temporally very precise across a wide range of temperatures in all three Drosophila species investigated. However, we find temperature dependence in the heterochronicity. A simple model incorporating history-dependence can explain the developmental temporal trajectories. Interestingly, the history-dependence is temperature specific with either effective negative or positive feedback at different temperatures. We also find that embryos are surprisingly robust to shifting temperatures during embryogenesis. We further identify differences between tropical and temperate species that are suggestive of different potential mechanisms regulating temporal development depending on the local environment. Overall, our data shows that Drosophila embryonic development is temporally robust across a wide range of temperatures but there are species specific differences.

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Section: Discussionmentioning

confidence: 86%

Temporal development of Drosophila embryos is highly robust across a wide temperature range

Chong

Amourda

Saunders

2018

Preprint

View full text Add to dashboard Cite

show abstract

“…However, more detailed species-specific analysis will be required to confirm this observation. Along these lines, a recent observation revealed that the Drosophila βtubulin97EF is upregulated at low temperatures and contributes to stabilize microtubules [ 29 ]. This example demonstrates that differential regulation of intracellular components is necessary for acclimation to environmental changes.…”

Section: Discussionmentioning

confidence: 99%

Temporal development of Drosophila embryos is highly robust across a wide temperature range

Chong

Amourda

Saunders

2018

J. R. Soc. Interface.

View full text Add to dashboard Cite

Development is a process precisely coordinated in both space and time. Spatial precision has been quantified in a number of developmental systems, and such data have contributed significantly to our understanding of, for example, morphogen gradient interpretation. However, comparatively little quantitative analysis has been performed on timing and temporal coordination during development. Here, we use Drosophila to explore the temporal robustness of embryonic development within physiologically normal temperatures. We find that development is temporally very precise across a wide range of temperatures in the three Drosophila species investigated. However, we find temperature dependence in the timing of developmental events. A simple model incorporating history dependence can explain the developmental temporal trajectories. Interestingly, history dependence is temperature-specific, with either effective negative or positive feedback at different temperatures. We also find that embryos are surprisingly robust to shifting temperatures during embryogenesis. We further identify differences between tropical and temperate species, potentially due to different mechanisms regulating temporal development that depend on the local environment. Our data show that Drosophila embryonic development is temporally robust across a wide range of temperatures. This robustness shows interesting species-specific differences that are suggestive of different sensitivity to temperature fluctuations between Drosophila species.

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“…Thus, it is impossible to conclude from the RNAi data about the unique role of β3Tub in cap cell morphogenesis. Given that the available loss-of-function alleles of β3Tub and β1Tub are lethal ( Myachina et al 2017 ), better genetic tools that allow cell-specific knockout of β3Tub or β1Tub within the ChO are required for distinguishing between the roles played by each of these tubulin isotypes. Additional tools are also required to allow for cap cell-specific knockdown of genes using RNAi transgenes.…”

Section: Discussionmentioning

confidence: 99%

An RNAi Screen Identifies New Genes Required for Normal Morphogenesis of Larval Chordotonal Organs

Hassan

Timerman

Hamdan

et al. 2018

G3 Genes|Genomes|Genetics

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The proprioceptive chordotonal organs (ChO) of a fly larva respond to mechanical stimuli generated by muscle contractions and consequent deformations of the cuticle. The ability of the ChO to sense the relative displacement of its epidermal attachment sites likely depends on the correct mechanical properties of the accessory (cap and ligament) and attachment cells that connect the sensory unit (neuron and scolopale cell) to the cuticle. The genetic programs dictating the development of ChO cells with unique morphologies and mechanical properties are largely unknown. Here we describe an RNAi screen that focused on the ChO’s accessory and attachment cells and was performed in 2nd instar larvae to allow for phenotypic analysis of ChOs that had already experienced mechanical stresses during larval growth. Nearly one thousand strains carrying RNAi constructs targeting more than 500 candidate genes were screened for their effects on ChO morphogenesis. The screen identified 31 candidate genes whose knockdown within the ChO lineage disrupted various aspects of cell fate determination, cell differentiation, cellular morphogenesis and cell-cell attachment. Most interestingly, one phenotypic group consisted of genes that affected the response of specific ChO cell types to developmental organ stretching, leading to abnormal pattern of cell elongation. The ‘cell elongation’ group included the transcription factors Delilah and Stripe, implicating them for the first time in regulating the response of ChO cells to developmental stretching forces. Other genes found to affect the pattern of ChO cell elongation, such as αTub85E, β1Tub56D, Tbce, CCT8, mys, Rac1 and shot, represent putative effectors that link between cell-fate determinants and the realization of cell-specific mechanical properties.

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Drosophila β-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules

Cited by 25 publications

References 78 publications

Temporal development of Drosophila embryos is highly robust across a wide temperature range

Temporal development of Drosophila embryos is highly robust across a wide temperature range

Temporal development of Drosophila embryos is highly robust across a wide temperature range

An RNAi Screen Identifies New Genes Required for Normal Morphogenesis of Larval Chordotonal Organs

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