<i>Drosophila rae1</i> is required for male meiosis and spermatogenesis

Volpi, Silvia; Bongiorni, Silvia; Fabbretti, Fabiana; Wakimoto, Barbara T.; Prantera, Giorgio

doi:10.1242/jcs.111328

Cited by 22 publications

(16 citation statements)

References 44 publications

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“…Rae1 is involved in mRNA export through the NPC. The observation that depletion of these two Nups resulted in the loss of meiotic cells was consistent with previously published results that meiosis did not occur in Nup154 mutant and Rae1-depleted testes [38,39]. On the other hand, the other three Nups of the Nup62 complex constructs the central channel in NPC.…”

Section: Depletion Of the Nup62 Complex Components Constructing The Csupporting

confidence: 92%

Nuclear Export of Cyclin B Mediated by the Nup62 Complex Is Required for Meiotic Initiation in Drosophila Males

Okazaki

Yamazoe

Inoué

2020

Cells

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Background: The central channel of the nuclear pore complex plays an important role in the selective transport of proteins between the nucleus and cytoplasm. Previous studies have demonstrated that the depletion of the Nup62 complex, constructing the nuclear pore channel in premeiotic Drosophila cells, resulted in the absence of meiotic cells. We attempted to understand the mechanism underlying the cell cycle arrest before meiosis. Methods: We induced dsRNAs against the nucleoporin mRNAs using the Gal4/UAS system in Drosophila. Results: The cell cycle of the Nup62-depleted cells was arrested before meiosis without CDK1 activation. The ectopic over-expression of CycB, but not constitutively active CDK1, resulted in partial rescue from the arrest. CycB continued to exist in the nuclei of Nup62-depleted cells and cells depleted of exportin encoded by emb. Protein complexes containing CycB, Emb, and Nup62 were observed in premeiotic spermatocytes. CycB, which had temporally entered the nucleus, was associated with Emb, and the complex was transported back to the cytoplasm through the central channel, interacting with the Nup62 complex. Conclusion: We proposed that CycB is exported with Emb through the channel interacting with the Nup62 complex before the onset of meiosis. The nuclear export ensures the modification and formation of sufficient CycB-CDK1 in the cytoplasm.

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Section: Depletion Of the Nup62 Complex Components Constructing The Csupporting

confidence: 92%

Nuclear Export of Cyclin B Mediated by the Nup62 Complex Is Required for Meiotic Initiation in Drosophila Males

Okazaki

Yamazoe

Inoué

2020

Cells

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“…It was recently shown that Drosophila RAE1 plays an essential role in male meiosis and spermatogenesis [30]. Fission yeast Rae1 may also promote meiosis by transporting mRNPs from the nucleus to the cytoplasm.…”

Section: Resultsmentioning

confidence: 99%

Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast

et al. 2014

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BackgroundMeiotic cells undergo two rounds of nuclear division and generate gametes. Previous studies have indicated that a number of transcription factors modulate the transcriptome in successive waves during meiosis and spore formation in fission yeast. However, the mechanisms underlying the post-transcriptional regulation in meiosis are not fully understood. The fission yeast spo5 + gene encodes a meiosis-specific RNA-binding protein, which is required for the progression of meiosis II and spore formation. However, the target RNA molecules of Spo5 are yet to be identified. Characterization of meiosis-specific RNA-binding proteins will provide insight into how post-transcriptional regulation influence gene expression during sexual differentiation.ResultsTo assess the functional significance of RNA-recognition motifs (RRMs) of Spo5, we constructed a series of new spo5 truncated mutants and previously reported spo5 missense mutants. In addition, we isolated novel spo5 missense mutants. The phenotypic characteristics of these mutants indicated that the RRMs are essential for both the localization and function of the protein. Interestingly, Spo5 is exported from the nucleus to the cytoplasm via the Rae1-dependent mRNA export pathway, but is unlikely to be involved in global mRNA export. Furthermore, cytoplasmic localization of Spo5 is important for its function, which suggests the involvement of Spo5 in post-transcriptional regulation. We identified pcr1 + mRNA as one of the critical targets of Spo5. The pcr1 + gene encodes an activating transcription factor/cAMP response element binding (ATF/CREB) transcription factor family. Among the four family members, namely Pcr1, Atf1, Atf21, and Atf31, only the mRNA encoding Pcr1 binds to Spo5.ConclusionsSpo5 is exported from the nucleus with mRNAs via the Rae1-dependent pathway. RRMs are necessary for this process and also for the function of Spo5 after the nuclear export. Spo5 appears to influence the activity of pcr1 + mRNA, and the mechanism of how Spo5 stimulates the mRNA to promote the progression of meiosis II and spore formation remains an intriguing question for future research.

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“…The same mtlncRNAs have also shown to possess binding affinity for nDNA regions coding for RNA export proteins that may function in nucleo-cytoplasmic RNA transport [53], neuroprotective and anti-apoptotic factors, and excitatory neurotransmitter receptors [54] and the dihydrofolate reductase (DHFR), a key enzyme in folate metabolism which also contributes to the maintenance of the mitochondrial integrity [55]. The mtlncRNAs are thus likely to significantly contribute to the regulation of the nuclear activity.…”

Section: Long Non-protein-coding Rnasmentioning

confidence: 99%

Mitochondrial Epigenetics and Environmental Exposure

Lambertini

Byun

2016

Curr Envir Health Rpt

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The rising toll of chronic and debilitating diseases brought about by the exposure to an ever expanding number of environmental pollutants and socio-economic factors is calling for action. The understanding of the molecular mechanisms behind the effects of environmental exposures can lead to the development of biomarkers that can support the public health fields of both early diagnosis and intervention to limit the burden of environmental diseases. The study of mitochondrial epigenetics carries high hopes to provide important biomarkers of exposure and disease. Mitochondria are in fact on the frontline of the cellular response to the environment. Modifications of the epigenetic factors regulating the mitochondrial activity are emerging as informative tools that can effectively report on the effects of the environment on the phenotype. Here, we will discuss the emerging field of mitochondrial epigenetics. This review describes the main epigenetic phenomena that modify the activity of the mitochondrial DNA including DNA methylation, long and short non-coding RNAs. We will discuss the unique pattern of mitochondrial DNA methylation, describe the challenges of correctly measuring it, and report on the existing studies that have analysed the correlation between environmental exposures and mitochondrial DNA methylation. Finally, we provide a brief account of the therapeutic approaches targeting mitochondria currently under consideration.

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Drosophila rae1 is required for male meiosis and spermatogenesis

Cited by 22 publications

References 44 publications

Nuclear Export of Cyclin B Mediated by the Nup62 Complex Is Required for Meiotic Initiation in Drosophila Males

Nuclear Export of Cyclin B Mediated by the Nup62 Complex Is Required for Meiotic Initiation in Drosophila Males

Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast

Mitochondrial Epigenetics and Environmental Exposure

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