<i>Ex Vivo</i> Bone Formation in Bovine Trabecular Bone Cultured in a Dynamic 3D Bioreactor Is Enhanced by Compressive Mechanical Strain

David, Véronique; Guignandon, Alain; Martin, Aline; Malaval, Luc; Lafage-Proust, Marie-Hélène; Rattner, Aline; Mann, V.; Noble, Brendon; Jones, David B.; Vico, Laurence

doi:10.1089/ten.a.2007.0051

Cited by 76 publications

(105 citation statements)

References 35 publications

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“…Consistently, dynamic compression enhances the vasculogenic activities of circulating endothelial precursors (EPCs) seeded in the demineralized bone matrix scaffolds (Kong et al, 2012). The relationship between mechanical inputs and resulting biological tissue structure, composition, and metabolism is reported and discussed in several other papers and reviews (David et al, 2008;Desmoulin et al, 2013;Gardel, Serra, Reis, & Gomes, 2014;Gaspar, Gomide, & Monteiro;S.-T. Li et al, 2014;van Griensven et al, 2009).…”

Section: The Need For Function: Mechano-electrical Stressmentioning

confidence: 91%

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

et al. 2015

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Bone plays several physiological functions and is the second most commonly transplanted tissue after blood. Since the treatment of large bone defects is still unsatisfactory, researchers have endeavoured to obtain scaffolds able to release growth and differentiation factors for mesenchimal stem cells, osteoblasts and endothelial cells in order to obtain faster mineralization and prompt a reliable vascularization.Nowadays, the application of osteoblastic cultures spans from cell physiology and pharmacology to cytocompatibility measurement and osteogenic potential evaluation of novel biomaterials. To overcome the simple traditional monocultures in vitro, co-cultures of osteogenic and vasculogenic precursors were introduced with very interesting results. Increasingly complex culture systems have been developed, where cells are seeded on proper scaffolds and stimulated so as to mimic the physiological conditions more accurately. These bioreactors aim at enabling bone regeneration by incorporating different cells types into bioinspired materials within a surveilled habitat.This review is focused on the most recent developments in the organomimetic cultures of osteoblasts and vascular endothelial cells for bone tissue engineering.

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Section: The Need For Function: Mechano-electrical Stressmentioning

confidence: 91%

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

et al. 2015

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“…Our original culture chamber was developed to be compatible with the Zetos ® compression device (David et al, 2008;Davies et al, 2006;Dumas et al, 2009) which G Bouet et al In vitro 3D bone culture model can exert cyclic compression on a mobile piston through a piezo-electric actuator controlled by a computer program (Fig. 1D).…”

Section: Mechanical Loadingmentioning

confidence: 99%

Validation of an in vitro 3D bone culture model with perfused and mechanically stressed ceramic scaffold

Bouet¹,

Cruel²,

Laurent³

et al. 2015

eCM

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An engineered three dimensional (3D) in vitro cell culture system was designed with the goal of inducing and controlling in vitro osteogenesis in a reproducible manner under conditions more similar to the in vivo bone microenvironment than traditional two-dimensional (2D) models. This bioreactor allows efficient mechanical loading and perfusion of an original cubic calcium phosphate bioceramic of highly controlled composition and structure. This bioceramic comprises an internal portion containing homogeneously interconnected macropores surrounded by a dense layer, which minimises fluid flow bypass around the scaffold. This dense and flat layer permits the application of a homogeneous loading on the bioceramic while also enhancing its mechanical strength. Numerical modelling of constraints shows that the system provides direct mechanical stimulation of cells within the scaffold. Experimental results establish that under perfusion at a steady flow of 2 µL/min, corresponding to 3 ≤ Medium velocity ≤ 23 µm/s, mouse calvarial cells grow and differentiate as osteoblasts in a reproducible manner, and lay down a mineralised matrix. Moreover, cells respond to mechanical loading by increasing C-fos expression, which demonstrates the effective mechanical stimulation of the culture within the scaffold. In summary, we provide a "proof-of-concept" for osteoblastic cell culture in a controlled 3D culture system under perfusion and mechanical loading. This model will be a tool to analyse bone cell functions in vivo, and will provide a bench testing system for the clinical assessment of bioactive bone-targeting molecules under load.

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“…Chick limb organ culture in the 21 st century perfusion of nutrients throughout the tissue (Bilodeau and Mantovani, 2006;Butler et al, 2000;David et al, 2008;El Haj and Cartmell, 2010;Mauney et al, 2004;Yu et al, 2004). A wide variety of bioreactor types exist that include rotating oxygen-diffusing vessels, filled with culture media, and perfusion systems incorporating controlled flow back and forth within the construct.…”

Section: El Smith Et Almentioning

confidence: 99%

A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation

Smith

Kanczler²,

Oreffo³

2013

eCM

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Scientific research and progress, particularly in the drug discovery and regenerative medicine fields, is typically dependent on suitable animal models to develop new and improved clinical therapies for injuries and diseases. In vivo model systems are frequently utilised, but these models are expensive, highly complex and pose a number of ethical considerations leading to the development and use of a number of alternative ex vivo model systems. The ex vivo embryonic chick long bone and limb bud models have been utilised in the scientific research field as a model to understand skeletal development for over eighty years. The rapid development of avian skeletal tissues, coupled with the ease of experimental manipulation, availability of genome sequence and the presence of multiple cell and tissue types has seen such model systems gain significant research interest in the last few years in the tissue engineering field. The models have been explored both as systems for understanding the developmental bone niche and as potential testing tools for tissue engineering strategies for bone repair and regeneration. This review details the evolution of the chick limb organ culture system and presents recent innovative developments and emerging techniques and technologies applied to these models that are aiding our understanding of skeletal developmental and regenerative medicine research and application.

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Ex Vivo Bone Formation in Bovine Trabecular Bone Cultured in a Dynamic 3D Bioreactor Is Enhanced by Compressive Mechanical Strain

Cited by 76 publications

References 35 publications

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

Overcoming physical constraints in bone engineering: ‘the importance of being vascularized’

Validation of an in vitro 3D bone culture model with perfused and mechanically stressed ceramic scaffold

A new take on an old story: chick limb organ culture for skeletal niche development and regenerative medicine evaluation

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