Ex vivo
 observation of
 Pythium insidiosum
 -antigen treated neutrophils on three
 Pythium insidiosum
 strains isolated from vascular pythiosis patients

Medhasi, Sadeep; Sriwarom, Apichaya; Permpalung, Nitipong; Torvorapanit, Pattama; Plongla, Rongpong; Chindamporn, Ariya; Worasilchai, Navaporn

doi:10.1080/21645515.2024.2304372

Human Vaccines & Immunotherapeutics

2024

DOI: 10.1080/21645515.2024.2304372

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Ex vivo observation of Pythium insidiosum -antigen treated neutrophils on three Pythium insidiosum strains isolated from vascular pythiosis patients

Sadeep Medhasi,

Apichaya Sriwarom,

Nitipong Permpalung

et al.

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Functional characterization of macrophages and change of Th1/Th2 balance in patients with pythiosis after Pythium insidiosum antigen immunotherapy

Medhasi,

Sangphech,

Permpalung

et al. 2024

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There has been limited research into the role of the Pythium insidiosum antigen (PIA) in modulating immune response in patients with pythiosis. This study investigated the balance of T helper type 2 (Th2) and T helper type 1 (Th1) responses after receiving PIA immunotherapy in patients with pythiosis. Next, the phagocytic activity and phagocytic index of IFN-γ primed PIA-treated macrophages were examined. Furthermore, the phagocytosis of infective P. insidiosum zoospores by macrophages was investigated. This work showed that the PIA vaccine induced Th1 response and M1 macrophages in patients with vascular pythiosis who survived and those with localized pythiosis. Phagocytic activity and phagocytic index were increased considerably in localized pythiosis patients compared to vascular pythiosis patients with hematological diseases. IFN-γ priming of PIA-treated macrophages against P. insidiosum zoospores enhanced the phagocytic activity and phagocytic index in vascular and localized pythiosis patients. Macrophages engulfed P. insidiosum zoospores, but the zoospores continued germination, resulting in macrophage death. Overall, our results suggest that PIA can modulate the immune responses, contributing to higher levels of Th1-type cytokine and potentially improving the survival of patients with vascular pythiosis. This study is the first to uncover that P. insidiosum zoospores can survive within macrophages. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-78756-x.

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Functional characterization of macrophages and change of Th1/Th2 balance in patients with pythiosis after Pythium insidiosum antigen immunotherapy

Medhasi,

Sangphech,

Permpalung

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

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Ex vivo observation of Pythium insidiosum -antigen treated neutrophils on three Pythium insidiosum strains isolated from vascular pythiosis patients

Cited by 1 publication

References 52 publications

Functional characterization of macrophages and change of Th1/Th2 balance in patients with pythiosis after Pythium insidiosum antigen immunotherapy

Functional characterization of macrophages and change of Th1/Th2 balance in patients with pythiosis after Pythium insidiosum antigen immunotherapy

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