<i>In locus</i>analysis of patterning evolution in the BMPR2 Wishful thinking

Marmion, Robert A.; Yakoby, Nir

doi:10.1242/dev.161083

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…In reverse, the AE of witF, which responds exclusively to Brk in its native context, responds to Brkindependent Smad activation in the context of the Dad CRM. Notably, our results are in agreement with a recent study focusing on evolutionary diversification of wit expression between Drosophila species as illustrated by differences in the width of the anterior wit stripe in D. melanogaster and D. virilis egg chambers (Marmion and Yakoby, 2018). Marmion and Yakoby independently identify Brk and the Brinker-binding site as the mediators of BMP-dependent wit expression.…”

Section: Discussionsupporting

confidence: 92%

Sequence environment of BMP-dependent activating elements controls transcriptional responses to Dpp signaling in Drosophila

et al. 2019

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Intercellular signaling pathways activate transcription factors, which, along with tissue-specific co-factors, regulate expression of target genes. Responses to TGFβ/BMP signals are mediated by Smad proteins, which form complexes and accumulate in the nucleus to directly bind and regulate enhancers of BMP targets upon signaling. In Drosophila, gene activation by BMP signaling often requires, in addition to direct input by Smads, the signal-dependent removal of the transcriptional repressor Brk. Previous studies on enhancers of BMP-activated genes have defined a BMP-responsive motif, the AE, which integrates activatory and repressive input by the Smad complex and Brk, respectively. Here, we address whether sequence variations within the core AE sequences might endow the motif with additional properties accounting for qualitative and quantitative differences in BMP responses, including tissue specificity of transcriptional activation and differential sensitivity to Smad and Brk inputs. By analyzing and cross-comparing three distinct BMPresponsive enhancers from the genes wit and Dad in two different epithelia, the wing imaginal disc and the follicular epithelium, we demonstrate that differences in the AEs contribute neither to the observed tissue-restriction of BMP responses nor to differences in the utilization of the Smad and Brk branches for transcriptional activation. Rather, our results suggest that the cis-environment of the BMPresponse elements not only dictates tissue specificity but also differential sensitivity to the two BMP mediators.

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Section: Discussionsupporting

confidence: 92%

Sequence environment of BMP-dependent activating elements controls transcriptional responses to Dpp signaling in Drosophila

et al. 2019

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Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components

et al. 2019

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BackgroundBMP signaling is involved in myriad metazoan developmental processes, and study of this pathway in Drosophila has contributed greatly to our understanding of its molecular and genetic mechanisms. These studies have benefited not only from Drosophila’s advanced genetic tools, but from complimentary in vitro culture systems. However, the commonly-used S2 cell line is not intrinsically sensitive to the major BMP ligand Dpp and must therefore be augmented with exogenous pathway components for most experiments.ResultsHerein we identify and characterize the responses of Drosophila ML-DmD17-c3 cells, which are sensitive to Dpp stimulation and exhibit characteristic regulation of BMP target genes including Dad and brk. Dpp signaling in ML-DmD17-c3 cells is primarily mediated by the receptors Put and Tkv, with additional contributions from Wit and Sax. Furthermore, we report complex regulatory feedback on core pathway genes in this system.ConclusionsNative ML-DmD17-c3 cells exhibit robust transcriptional responses to BMP pathway induction. We propose that ML-DmD17-c3 cells are well-suited for future BMP pathway analyses.Electronic supplementary materialThe online version of this article (10.1186/s12861-019-0181-0) contains supplementary material, which is available to authorized users.

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The ETS-transcription factor Pointed is sufficient to regulate the posterior fate of the follicular epithelium

et al. 2020

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The Janus-kinase/Signal Transducers and Activators of Transcription (JAK/STAT) pathway regulates the anterior posterior axis of the Drosophila follicle cells. In the anterior, it activates the bone morphogenetic protein (BMP) signaling pathway through expression of the BMP ligand, decapentaplegic (dpp). In the posterior, JAK/STAT works with the epidermal growth factor receptor (EGFR) pathway to express the T-box transcription factor midline (mid). While MID is necessary in establishing the posterior fate of the egg chamber, we show that it is not sufficient to determine a posterior fate. The ETS-transcription factor pointed (pnt) is expressed in an overlapping domain to mid in the follicle cells. This study shows that pnt is upstream of mid, and it is sufficient to induce a posterior fate in the anterior end, which is characterized by the induction of mid, the prevention of the stretched cells formation, and the abrogation of border cells migration. We demonstrate that the anterior BMP signaling is abolished by PNT through dpp repression. However, ectopic DPP cannot rescue this repression, suggesting additional targets of PNT participate in the posterior fate determination.

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In locusanalysis of patterning evolution in the BMPR2 Wishful thinking

Cited by 3 publications

References 47 publications

Sequence environment of BMP-dependent activating elements controls transcriptional responses to Dpp signaling in Drosophila

Sequence environment of BMP-dependent activating elements controls transcriptional responses to Dpp signaling in Drosophila

Drosophila ML-DmD17-c3 cells respond robustly to Dpp and exhibit complex transcriptional feedback on BMP signaling components

The ETS-transcription factor Pointed is sufficient to regulate the posterior fate of the follicular epithelium

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