In vitroantifungal activity of extracts obtained fromHypericum perforatumadventitious roots cultured in a mist bioreactor against planktonic cells and biofilm ofMalassezia furfur

Simonetti, Giovanna; Tocci, Noemi; Valletta, Alessio; Brasili, Elisa; D’Auria, Felicia Diodata; Idoux, Alicia; Pasqua, Gabriella

doi:10.1080/14786419.2015.1028059

Cited by 40 publications

(28 citation statements)

References 14 publications

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“…Extracts from its aerial parts are used internally for the treatment of depression, which affects more than 300 million people worldwide, and externally for the healing of skin disorders (W€ olfle et al, 2014;Apaydin et al, 2016;WHO, 2017). In addition, extracts from the roots are under study for their pronounced antifungal activity against human pathogens, including Candida species (Tocci et al, 2011(Tocci et al, , 2013aSimonetti et al, 2016). H. perforatum contains a number of bioactive constituents, which act additively and/or synergetically (Schmidt & Butterweck, 2015).…”

Section: Introductionmentioning

confidence: 99%

Exodermis and endodermis are the sites of xanthone biosynthesis in Hypericum perforatum roots

et al. 2017

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Xanthones are specialized metabolites with antimicrobial properties, which accumulate in roots of Hypericum perforatum. This medicinal plant provides widely taken remedies for depressive episodes and skin disorders. Owing to the array of pharmacological activities, xanthone derivatives attract attention for drug design. Little is known about the sites of biosynthesis and accumulation of xanthones in roots. Xanthone biosynthesis is localized at the transcript, protein, and product levels using in situ mRNA hybridization, indirect immunofluorescence detection, and high lateral and mass resolution mass spectrometry imaging (AP-SMALDI-FT-Orbitrap MSI), respectively. The carbon skeleton of xanthones is formed by benzophenone synthase (BPS), for which a cDNA was cloned from root cultures of H. perforatum var. angustifolium. Both the BPS protein and the BPS transcripts are localized to the exodermis and the endodermis of roots. The xanthone compounds as the BPS products are detected in the same tissues. The exodermis and the endodermis, which are the outermost and innermost cell layers of the root cortex, respectively, are not only highly specialized barriers for controlling the passage of water and solutes but also preformed lines of defence against soilborne pathogens and predators.

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Section: Introductionmentioning

confidence: 99%

Exodermis and endodermis are the sites of xanthone biosynthesis in Hypericum perforatum roots

et al. 2017

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“…Antifungal susceptibility of M. furfur was determined according to standardized methods for yeast using the broth microdilution method with some modifications (CLSI M27‐A3, 2008; CLSI, ; Simonetti et al, ). Dermatophytes were grown on potato dextrose agar (Sigma Aldrich, St. Louis, Missouri, USA) at 28–30 °C until good conidial growth was present.…”

Section: Methodsmentioning

confidence: 99%

Anti‐Dermatophyte and Anti‐Malassezia Activity of Extracts Rich in Polymeric Flavan‐3‐ols Obtained from Vitis vinifera Seeds

Simonetti

D’Auria

Mulinacci

et al. 2016

Phytotherapy Research

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Several human skin diseases are associated with fungi as dermatophytes and Malassezia. Skin mycoses are increasing and new alternatives to conventional treatments with improved efficacy and/or safety profiles are desirable. For the first time, the anti-dermatophytes and the anti-Malassezia activities of Vitis vinifera seed extracts obtained from different table and wine cultivars have been evaluated. Geometric minimal inhibitory concentration ranged from 20 to 97 µg/mL for dermatophytes and from 32 to 161 µg/mL for Malassezia furfur. Dried grape seed extracts analyzed by HPLC/DAD/ESI/MS showed different quali-quantitative compositions in terms of monomeric and polymeric flavan-3-ols. The minimal inhibitory concentrations for Trichophyton mentagrophytes and for M. furfur were inversely correlated with the amount of the polymeric fraction (r = -0.7639 and r = -0.7228, respectively). Differently, the antifungal activity against T. mentagrophytes was not correlated to the content of flavan-3-ol monomers (r = 0.2920) and only weakly correlated for M. furfur (r = -0.53604). These results suggest that extracts rich in polymeric flavan-3-ols, recovered from V. vinifera seeds, could be used for the treatment of skin fungal infections. Copyright © 2016 John Wiley & Sons, Ltd.

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“…To overcome these drawbacks, special bioreactor setups were reported like gas phase or mist bioreactors. Simonetti et al [65] showed the possibility of cultivating SJW root cultures in mist bioreactors for 80 days. However, the authors focused mainly on the production of a special antifungal extract without detailed growth kinetics.…”

Section: Scale-up Potential In Bioreactormentioning

confidence: 99%

Toward enhanced hyperforin production in St. John's wort root cultures

Gaid

Grosch

Möller

et al. 2019

Engineering in Life Sciences

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During the past decades, several trials targeted a stable, sustainable and economic production of St. John's wort (Hypericum perforatum) extract. The value of this extract stems from its use to treat depression and skin irritation due to its hyperforin content. Previously, hyperforin‐forming in vitro root cultures were established. Here, detailed growth and production kinetics have been analyzed over 40 days of cultivation. In the first 10 days, sucrose was completely hydrolyzed to glucose and fructose. The ammonium consumption supported the increase in the biomass and hyperforin production. When sucrose was replaced with glucose/fructose, the linear growth phase started 6 days earlier and resulted in a higher space‐time‐yield. The maximum hyperforin production was 0.82 mg L−1 day−1, which was 67 % higher than in the sucrose‐supplemented standard cultivation. Buffering the sucrose‐supplemented medium with phosphate caused a 2.7‐fold increase in the product to biomass yield coefficient. However, the combination of monosaccharides and buffering conditions did not cause an appreciable improvements in the production performance of the shake flask approaches. A potential scalability from flask to lab‐scale stirred bioreactors has been demonstrated. The results obtained offer a basis for a scalable production of hyperforin and a sustainable source for a tissue culture‐based phytomedicine.

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In vitroantifungal activity of extracts obtained fromHypericum perforatumadventitious roots cultured in a mist bioreactor against planktonic cells and biofilm ofMalassezia furfur

Cited by 40 publications

References 14 publications

Exodermis and endodermis are the sites of xanthone biosynthesis in Hypericum perforatum roots

Exodermis and endodermis are the sites of xanthone biosynthesis in Hypericum perforatum roots

Anti‐Dermatophyte and Anti‐Malassezia Activity of Extracts Rich in Polymeric Flavan‐3‐ols Obtained from Vitis vinifera Seeds

Toward enhanced hyperforin production in St. John's wort root cultures

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