<i>In vitro</i> Experimental Research for Using the Silver Nanoparticles as Plasmid Curing Agent in Some Types of Multi-Antibiotic Resistant Pathogenic Bacteria

Mhawesh, Ahmed A.; Owaif, Hasan A. Aal; Abdulateef, Sura A.

doi:10.5958/0976-5506.2019.02233.2

Cited by 5 publications

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“…The strains and plasmids used during this study werelisted in Table (1).Bacteria were routinely cultured in Luria-Bertani (LB) medium at 37°C withshaking at 200rpm, and supplemented with the appropriate antibiotic as required (50μg/ml kanamycin; 100μg/ml ampicillin; 35μg/ml chloramphenicol). During the experiments, a starter culture was grown overnight in LB then diluted 1:100 into fresh media and grown with shaking at 200rpm at 37°C, and the desired growth phase was determined by optical density at a wavelength of 600nm (OD 600 nm).…”

Section: Bacterial Strains Plasmids and Growth Conditionsmentioning

confidence: 99%

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The role of BipA in the regulation of K1 capsular polysaccharide production of uropathogenic Escherichia coli

Owaif¹,

Mhawesh²,

Abdulateef³

2019

ATMPH

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Background: K1 capsular polysaccharide is usually expressed in uropathogenic E. coli.The aim of current study was to evaluate the effect of BipA in the regulation of K1 capsular polysaccharide expression in E. coli. Methods: In order to identify the role of BipA in theK1 capsule expression,bipA gene was deletedin the UPEC strain UTI89.Results: The flow cytometry analyses showed that the capsular polysaccharide expressedon the UTI89ΔbipA cells was less thanthe capsular polysaccharide present on the wild type UTI89 cells. Also, plaque assays using K1specific bacteriophage showed that the plaque diameter produced on UTI89ΔbipA was about 1/2 the plaque diameter produced on wild typeUTI89indicated a reduction in the capsular polysaccharide expression from UTI89ΔbipA in comparison with UTI89.Conclusion: These results showed that the expression of K1 capsular polysaccharide is upregulated by BipA.

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Section: Bacterial Strains Plasmids and Growth Conditionsmentioning

confidence: 99%

“…Most of the urinary tractinfections (UTIs) are caused by Uropathogenic Escherichia coli (UPEC) [1] . UPEC strains have both structural and secreted virulence factors that are required to cause disease [2,3] .…”

Section: Introductionmentioning

confidence: 99%

The role of BipA in the regulation of K1 capsular polysaccharide production of uropathogenic Escherichia coli

Owaif¹,

Mhawesh²,

Abdulateef³

2019

ATMPH

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“…Today, the increasing spread of antibiotic-resistant bacteria is one of the problems of human society. Bacteria can be resistant to different types of antibiotics through different mechanisms [10][11][12][13] . One of the most common antibiotic resistance mechanisms, especially in Gram-negative bacteria, is the production of beta-lactamase enzymes.…”

Section: Introductionmentioning

confidence: 99%

Major Genetic Determinants of Extended-Spectrum Β-Lactamase (ESBL), Carbapenemase, Fosfomycin and Colistin Resistance in Escherichia Coli from Intensive Care Units

Mhawesh

khudair

Abbas³

2022

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Escherichia coli (E. coli) strains placed in predominant nosocomial bacteria in intensive care units (ICUs), resulting in severe drug-resistant infections. Non-susceptibility to β-lactams and last-line drugs such as Fosfomycin and Colistin cause limited availability of infections eradication. The objective of this study included the determination of genes encoding extended-spectrum β-lactamase (ESBL), Carbapenemase, Colistin, and Fosfomycin resistance in clinical isolates of E. coli in ICUs. A total of 200 E. coli isolates were identified from ICU settings. The CTXM-1, SHV, IMP and OXA-48 genes were detected for β-lactamases using the polymerase chain reaction (PCR) technique. The fosA3 and mcr-1 and mcr-2 genes were also detected for resistance against Fosfomycin and Colistin. The CTX-M1, SHV, IMP and OXA-48 genes were detected in 60 (30%), 56 (28%), 28 (14%) and 4 (8%) of isolates. none of the E. coli isolates had the mcr-2 and fosA3 genes. Despite the existence of resistance genes to the third-generation antibiotics and Carbapenemase s, any isolates had genes for resistance to Fosfomycin and Colistin. More studies are needed to follow the resistance genes against last-resort antibiotics.

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“…In recent years, Enterococcus species have been identified as the major contributing pathogens in hospital infections and community-acquired infections 1,2 , due to the acquisition of multiple antibiotic resistance genes, such as beta-lactams, cephalosporins, trimethoprim-sulfamethoxazole and glycopeptides. Some Enterococci are inherently resistant to beta-lactam, and aminoglycoside antibiotics are considered a medical crisis [3][4][5][6] . Glycopeptides like vancomycin are used as the last line of antibiotics in treating multidrug-resistant (MDR) Gram-positive bacteria 7 .…”

Section: Introductionmentioning

confidence: 99%

“…The Enterococci pathogenic spp are less dependent on their virulence factors and the ability to adapt to environmental conditions 8,10 . Their resistance to antibiotics and ecological stresses, binding properties, and biofilm formation has resulted in crucial pathogenic features associated with implant infections 5,6,8 . In addition, Enterococci are significant contributors to nosocomial infections, and biofilm formation, which employ a wide variety of factors, including genetic factors, various environmental conditions, and signals mostly unknown and required for identification and characterization 11,12.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Some Vancomycin and Virulence Factor Genes in Enterococcus faecium

Mhawesh

Khalaf

khudair

2022

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Multidrug-resistant (MDR) and pathogenic E. faecium is a crisis in healthcare settings. This survey aimed at antibiotic susceptibility profiling and virulence determinants of E. faecium. This study pooled 100 fecal E. faecium isolates identified by phenotypic and molecular tests. Antibiotic susceptibility and ampicillin MIC were determined according to clinical and laboratory standards institute (CLSI) 2017. Moreover, biofilm formation was assayed by a microtiter tissue plate assay. Virulence genes pattern was performed by polymerase chain reaction (PCR) method. Among 460 fecal samples, 100 isolates of E. faecium were identified, among which the highest resistance was related to penicillin (81%), cephalothin (78%), cefazolin (76%), tetracycline and cefepime (69%). In contrast, 83% of them were susceptible to vancomycin. Moreover, four vancomycin-resistant isolates had vancomycin MIC>32µg/mL, and 11 isolates had MIC>8µg/mL. Of 32 ampicillin-resistant isolates, 18 (56%) produced strong biofilm, and 14 isolates (44%) produced moderate biofilm. Among 17 vancomycin-resistant E. faecium (VREfa), 15 (88.23%) isolates produced strong biofilms, and two of them produced moderate-level biofilms, which was significantly higher than susceptible isolates (p=0.0144). The vanA (vancomycin MIC: 16-64µg/mL) and vanB (vancomycin MIC: 8-64µg/mL) genes were detected in twelve and five isolates, respectively. The rate of adhesin genes ace, esp and ebp included 68%, 97% and 82%, respectively. All the VREfa harbored the ace, esp and ebp genes. The antibiotic resistance rate of E. faecium was low. Biofilm formation was significantly different between VREfa and vancomycin-susceptible isolates but not between k9ampicillin-resistant and ampicillin-susceptible isolates. All the VREfa harbored the ace, esp and ebp genes. The virulence adhesin genes were not significantly associated with biofilm formation. Further studies are essential to appreciate better the relation between biofilm formation, drug non-susceptibility and adhesin genes. Keywords: E. faecium, antibiotic resistance, biofilm formation, virulence genes

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In vitro Experimental Research for Using the Silver Nanoparticles as Plasmid Curing Agent in Some Types of Multi-Antibiotic Resistant Pathogenic Bacteria

Cited by 5 publications

References 0 publications

The role of BipA in the regulation of K1 capsular polysaccharide production of uropathogenic Escherichia coli

The role of BipA in the regulation of K1 capsular polysaccharide production of uropathogenic Escherichia coli

Major Genetic Determinants of Extended-Spectrum Β-Lactamase (ESBL), Carbapenemase, Fosfomycin and Colistin Resistance in Escherichia Coli from Intensive Care Units

Molecular Detection of Some Vancomycin and Virulence Factor Genes in Enterococcus faecium

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