<i>In Vitro</i>Fertilization

Hasler, J.F.; Barfield, Jennifer P.

doi:10.1002/9781118833971.ch81

Cited by 3 publications

(3 citation statements)

References 99 publications

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“…Previously conducted research works indicated that repeated collection oocytes via flank incision from living donor cows resulted in high recovery rates. Nevertheless, the method was found to be expensive, inefficient, and associated with formation of adhesions and subsequent loss of fertility [119,73]. In the contrary, repeated transvaginal follicular aspiration sessions can be performed in cattle without significant side effects and with a minimal stress to the animal [120].…”

Section: Percoll Density Gradient Methodmentioning

confidence: 99%

“…Such collection schemes can be carried out for longer periods, where recovery of about four to five quality oocytes per each collection session can be achieved [71]. According to Alnimer [72], a number of factors such as breed of cattle, age of donor, number of follicles present, frequency of collection, and vacuum pressure used to retrieve the follicular fluid, size and type of aspirating needle affect the number of intact COCs collected [73]. Manipulation of follicular dynamics [74] and function through hormonal treatment and dietary supplementation with energy concentrates that are rich in fatty acids [75] would help to improve oocyte quality within the follicle in OPU-IVF programs.…”

Section: Sources Of Oocyte For In Vitro Embryomentioning

confidence: 99%

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Assisted Reproductive Technologies and Recent Developments in In vitro Embryo Production Technology in Dairy Cattle

Yilma

2022

AJRAVS

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Scientific and technological researches in livestock reproduction have culminated in the development of various reproductive techniques. Assisted reproductive technologies that include artificial insemination, superovulation, estrus synchronization, in vitro fertilization and semen sexing as well as gametes and embryo cryopreservation have a major effect on dairy cattle genetic improvement programs worldwide. The major efforts of assisted reproductive technologies are production of large number of embryos from genetically superior dams and sires and dissemination of genetic material all over the world. To this end, scientific advances in in vitro fertilization and embryo production immensely contributed to the genetic improvement in cattle production systems. In vitro fertilization with sexed semen has been successfully used to enhance genetic improvement in wider part of the world. Recent developments in reproductive technologies which include ultrasound guided oocytes aspiration, semen and embryo sexing and cryopreservation of embryos immensely contributed for the advancement and success of reproduction and production in dairy industries worldwide.

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Section: Percoll Density Gradient Methodmentioning

confidence: 99%

Section: Sources Of Oocyte For In Vitro Embryomentioning

confidence: 99%

Assisted Reproductive Technologies and Recent Developments in In vitro Embryo Production Technology in Dairy Cattle

Yilma

2022

AJRAVS

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“…However, in vitro fertilization, IVF, is frequently used as a general term for the process of creating embryos outside of the body, which includes in vitro maturation (IVM) of oocytes, in vitro insemination and fertilization of oocytes (IVF) and in vivo culture (IVC) of resulting embryos. The steps of IVP are conducted in the sequence IVM-IVF-IVC (Hasler and Barfield, 2015). Assisted reproductive technologies, or ART, encompasses all the procedures that could be used to complete IVP.…”

Section: List Of Abbreviationsmentioning

confidence: 99%

41 Effects of Dimethyl Sulfoxide- or Glycerol-Based Vitrification Protocols on Zona Pellucida Hardening in Mature Bovine Oocytes

Rogers

Foster

Guiterrez

et al. 2018

Reprod. Fertil. Dev.

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Zona pellucida hardening is a natural process that occurs after oocyte fertilization to prevent polyspermic fertilization and to protect embryonic development. Pre-fertilization hardening of the zona pellucida, however, decreases fertilization rates. Cryoprotectants have also been shown to negatively affect fertilization rates, one possible mechanism of which being through zona hardening. This experiment was conducted to determine the effect of different cryoprotectants on hardening of the zona pellucida of mature bovine oocytes. Oocytes were collected by ovum pick-up (OPU) by transvaginal ultrasound guided aspiration (TUGA) from mixed-breed cows. After collection, oocytes were randomly assigned to 3 cryoprotectant treatment groups: dimethyl sulfoxide (DMSO), glycerol, or PBS (control). Drops (50 µL) of each vitrification solution were placed under mineral oil. Vitrification solution 1 (VS1) contained 10% ethylene glycol (EG), either 10% DMSO or glycerol, and 0.5 M sucrose. Vitrification solution 2 (VS2) contained 20% EG, 20% DMSO or glycerol, and 0.5 M sucrose. All oocytes were held in VS1 for 5 min before being transferred to VS2 for 45 s. All oocytes were washed in a common dilution solution (80% PBS, 20% calf serum, 0.025 M sucrose) for 5 min. Next, oocytes were moved to 50-µL drops of protease solution (0.1% protease) under mineral oil. Control oocytes were held in PBS for ~10 min before entering the protease solution to represent the same period as the vitrification procedure. The oocytes were observed until the zonae pellucidae were completely digested and times were recorded for each oocyte. This experiment included 4 replicates with a total of 88 oocytes used, 32 each in DMSO and glycerol and 24 in PBS. The data were analysed using ANOVA. The DMSO group had the lower mean zona digestion time out of the 2 cryoprotectants at 15.75 min and glycerol had the highest mean digestion time at 19.3 min. The control group (PBS) had the lowest mean of the 3 treatments at 12.7 min. The differences between DMSO and glycerol, and between DMSO and PBS were not significant (P = 0.0654 and 0.1073, respectively). However, both glycerol versus PBS and the average of DMSO and glycerol versus PBS were significantly different (P-value = 0.0053 and 0.0119, respectively). These results suggest that glycerol hardens the zona pellucida more than DMSO or PBS; however, there is not enough evidence to determine whether DMSO hardens the zona pellucida compared with PBS. This would suggest that, in relation to zona hardening and ensuring proper fertilization, glycerol-based cryoprotectants may be a better option than DMSO-based ones. Further, these results may be important in embryo vitrification as zona hardening may prevent blastocyst hatching, suggesting that glycerol-based cryoprotectants should be investigated as the optimal cryoprotectant here also.

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Guía de manejo de oocitos bovinos durante su colecta y envío para la producción in vitro de embriones

Cortés Escobar,

Gómez López,

Martínez Rocha

2023

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El nacimiento de un ternero sano, a partir de un embrión implantado en una vaca receptora y que ha sido producido en condiciones de laboratorio, es el resultado de un complejo y coordinado proceso que involucra el trabajo colaborativo entre productores, profesionales de campo y de laboratorio. Durante la formación del embrión, el oocito no solo aporta la mitad del material genético, sino que también crea un entorno esencial para el éxito del desarrollo embrionario. Por lo tanto, las características de los oocitos seleccionados y el manejo que se le dé a los mismos, son factores que influyen directamente en la calidad de los embriones obtenidos en el laboratorio. La finalidad de esta cartilla es proporcionar una guía detallada con ilustraciones y recomendaciones prácticas que resaltan puntos importantes del proceso de búsqueda, selección, clasificación, embalaje y envío de los oocitos al laboratorio para realizar la producción in vitro de embriones bovinos.

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In VitroFertilization

Cited by 3 publications

References 99 publications

Assisted Reproductive Technologies and Recent Developments in In vitro Embryo Production Technology in Dairy Cattle

Assisted Reproductive Technologies and Recent Developments in In vitro Embryo Production Technology in Dairy Cattle

41 Effects of Dimethyl Sulfoxide- or Glycerol-Based Vitrification Protocols on Zona Pellucida Hardening in Mature Bovine Oocytes

Guía de manejo de oocitos bovinos durante su colecta y envío para la producción in vitro de embriones

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