<i>In vitro</i> generation of differentiated cardiac myofibers on micropatterned laminin surfaces

McDevitt, Todd C.; Angello, John C.; Whitney, Marsha L.; Reinecke, Hans; Hauschka, Stephen D.; Murry, Charles E.; Stayton, Patrick S.

doi:10.1002/jbm.1292

Cited by 178 publications

(145 citation statements)

References 27 publications

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“…McDevitt et al 225 microstamped laminin lines (5-50 μm wide) on glass and on a biodegradable polymer (Fig. 3I).…”

Section: Micropatterns Of Cardiacmentioning

confidence: 99%

“…When cells were seen bridging across narrow gaps (10 μm), different lanes beat in synchrony. 225 Calcium-induced calcium release (CICR) is a critical mechanism for cardio-myocyte contraction. Kaji et al 226 patterned chick embryonic cardiac myocytes on fibronectin patterns microstamped on an octadecyl trichlorosilane SAM on glass.…”

Section: Micropatterns Of Cardiacmentioning

confidence: 99%

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Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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“…McDevitt et al 225 microstamped laminin lines (5-50 μm wide) on glass and on a biodegradable polymer (Fig. 3I).…”

Section: Micropatterns Of Cardiacmentioning

confidence: 99%

Section: Micropatterns Of Cardiacmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

View full text Add to dashboard Cite

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“…Spatial control was introduced by McDevitt et al (22,23). High densities of cardiomyocytes were obtained by relying on self-aggregation of cardiomyocytes, the basis for scaffoldfree systems (3)(4)(5)(6)(7)(8).…”

Section: Arrows) (D) Cd68 + Macrophages Infiltrated Porous Constructmentioning

confidence: 99%

Proangiogenic scaffolds as functional templates for cardiac tissue engineering

Madden¹,

Mortisen

Sussman³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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607

486

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We demonstrate here a cardiac tissue-engineering strategy addressing multicellular organization, integration into host myocardium, and directional cues to reconstruct the functional architecture of heart muscle. Microtemplating is used to shape poly(2-hydroxyethyl methacrylate-co-methacrylic acid) hydrogel into a tissue-engineering scaffold with architectures driving heart tissue integration. The construct contains parallel channels to organize cardiomyocyte bundles, supported by micrometer-sized, spherical, interconnected pores that enhance angiogenesis while reducing scarring. Surfacemodified scaffolds were seeded with human ES cell-derived cardiomyocytes and cultured in vitro. Cardiomyocytes survived and proliferated for 2 wk in scaffolds, reaching adult heart densities. Cardiac implantation of acellular scaffolds with pore diameters of 30-40 μm showed angiogenesis and reduced fibrotic response, coinciding with a shift in macrophage phenotype toward the M2 state. This work establishes a foundation for spatially controlled cardiac tissue engineering by providing discrete compartments for cardiomyocytes and stroma in a scaffold that enhances vascularization and integration while controlling the inflammatory response.angiogenesis | cardiomyocyte | human embryonic stem cell | hydrogel

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“…The orientation and cardiomyocyte phenotype could also be improved by microcontact printing of extracellular matrix components (e.g. laminin) on thin polyurethane and PLA films [20,21].…”

Section: Introductionmentioning

confidence: 99%

Interactive effects of surface topography and pulsatile electrical field stimulation on orientation and elongation of fibroblasts and cardiomyocytes

Au¹,

Cheng²,

Chowdhury³

et al. 2007

Biomaterials

178

166

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In contractile tissues such as myocardium, functional properties are directly related to the cellular orientation and elongation. Thus, tissue engineering of functional cardiac patches critically depends on our understanding of the interaction between multiple guidance cues such as topographical, adhesive or electrical. The main objective of this study was to determine the interactive effects of contact guidance and electrical field stimulation on elongation and orientation of fibroblasts and cardiomyocytes, major cell populations of the myocardium. Polyvinyl surfaces were abraded using lapping paper with grain size 1 to 80μm, resulting in V-shaped abrasions with the average abrasion peak-to-peak width in the range from 3 to 13μm, and the average depth in the range from 140nm to 700nm (AFM). The surfaces with abrasions 13μm wide and 700nm deep, exhibited the strongest effect on neonatal rat cardiomyocyte elongation and orientation as well as statistically significant effect on orientation of fibroblasts, thus they were utilized for electrical field stimulation. Electrical field stimulation was performed using a regime of relevance for heart tissue in vivo as well as for cardiac tissue engineering. Stimulation (square pulses, 1ms duration, 1Hz, 2.3V/cm or 4.6V/cm) was initiated 24hr after cell seeding and maintained for additional 72hr. The cover slips were positioned between the carbon rod electrodes so that the abrasions were either parallel or perpendicular to the field lines. Non-abraded surfaces were utilized as controls. Field stimulation did not affect cell viability (live/dead staining). The presence of a well developed contractile apparatus in neonatal rat cardiomyocytes (staining for cardiac Troponin I and actin filaments) was identified in the groups cultivated on abraded surfaces in the presence of field stimulation. Overall we observed that i) fibroblast and cardiomyocyte elongation on non-abraded surfaces was significantly enhanced by electrical field stimulation ii) electrical field stimulation promoted orientation of fibroblasts in the direction perpendicular to the field lines when the abrasions were also placed perpendicular to the field lines and iii) topographical cues were a significantly stronger determinant of cardiomyocyte orientation than the electrical field stimulation. The orientation and elongation response of cardiomyocytes was completely abolished by inhibition of actin polymerization (Cytochalasin D) and only partially by inhibition of phosphatidyl-inositol 3 kinase (PI3K) pathway (LY294002).

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In vitro generation of differentiated cardiac myofibers on micropatterned laminin surfaces

Cited by 178 publications

References 27 publications

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Proangiogenic scaffolds as functional templates for cardiac tissue engineering

Interactive effects of surface topography and pulsatile electrical field stimulation on orientation and elongation of fibroblasts and cardiomyocytes

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