In vitroimmunomodulatory effects of fractions obtained from aqueous extracts ofLarrea divaricataCav (Jarilla) on mouse peritoneal macrophages

Abstract

These results indicate that NDGA was not the compound responsible for the immunomodulatory action exerted by the aqueous extract from L. divaricata.

In vivo effect of three fractions of Larrea divaricata Cav. (jarilla) on the innate immune system: macrophage response against Candida albicans

“…We also demonstrated that L. divaricata decoction has immunomodulatory activity both in vitro and in vivo 7,8 . Moreover, fractions isolated from the decoction induced the activation of the innate immune system in vitro 9 . On the other hand, Davicino et al.…”

“…Fractions were prepared as described previously 9 . Briefly, 7 g of lyophilised material was resuspended in chloroform and chromatographed in a column of silica gel (130 gr) using different chloroform/methanol ratios (97.5 : 2.5; 95 : 5; 92.5 : 7.5; 90 : 10; 80 : 20 and pure methanol) as the mobile phase as described.…”

“…All the portions with similar profiles were pooled. Five fractions (F1–F5) were obtained and dried 9 . The dry weights of the fractions were: F1: 118 mg; F2: 288.11 mg; F3: 56 mg, F4: 72 mg and F5: 315 mg. Only F1–F3 were used in cell studies, as in previous reports 9 .…”

“…Five fractions (F1–F5) were obtained and dried 9 . The dry weights of the fractions were: F1: 118 mg; F2: 288.11 mg; F3: 56 mg, F4: 72 mg and F5: 315 mg. Only F1–F3 were used in cell studies, as in previous reports 9 . Before assays, all drugs and extracts used were subjected to the Limulus test for the detection of lipopolysaccharide (LPS) contamination.…”

“…Apoptosis was evaluated after staining with Giemsa and acridine orange/ethidium bromide. After incubation, treated and untreated cells were stained with Giemsa 7 or 10 μg ml −1 acridine orange/ethidium bromide 9 . Cells were counted with an Axiovert inverted microscope (Carl Zeiss MicroImaging, Jena, Germany) using an excitation filter of 480/30 nm.…”