IN VITRO STIMULATION OF BLOOD LYMPHOCYTES FROM MYCOPLASMA PNEUMONIAE INFECTED PATIENTS WITH PNEUMONIA AND WITH DISORDERS OF THE CENTRAL NERVOUS SYSTEM

Abstract

stimulation of blood lymphocytes from Mycoplasrna pneurnoniae infected patients with pneumonia and with disorders of the central nervous system. Acta path. microbiol. scand. Sect. C, 88: 61-65, 1980. In 23 patients with Mycoplasma pneurnoniae (MP) infection ( I 3 with pneumonia and 10 with an acute febrile, non-bacterial disorder of the central nervous system (CNS)) and in 26 healthy control persons, thymidine incorporation of blood lymphocytes stimulated in vitro by killed MP was studied. The lymphocyte response to MP was significantly higher in the pneumonia patients than in the controls. In the patients with an acute disorder of the CNS, lymphocyte responses to MP tended to be low or normal in lack of pleocytosis in the spinal fluid, but were predominantly high when either pleocytosis or a pulmonary infiltrate was present. Lymphocyte responses to the mitogens PHA, PWM and Con-A were normal in all groups. The lack of increased responses to MP antigen in some of the neurological patients. despite a current MP infection, may reflect an antigen-specific depression or a lack of specific sensitization of their lymphocytes.

Sequential Mycoplasma pneumoniae pneumonia and Chromobacterium violaceum skin abscess in a pediatric patient

“…In addition, blood lymphocytes from patients in acute phase of Mycoplasma pneumoniae infection have been shown to have a decreased response to purified protein derivatives in vitro [26]. Hence, it has been suggested that Mycoplasma pneumoniae infection causes transient depression of cell-mediated immunity [26]. It appears possible that the depressed cell-mediated immunity during the acute Mycoplasma pneumoniae infection predisposed our patient to the development of Chrombobacterium violaceum infection which manifested as skin abscess.…”

“…In addition, blood lymphocytes from patients in acute phase of Mycoplasma pneumoniae infection have been shown to have a decreased response to purified protein derivatives in vitro [26]. Hence, it has been suggested that Mycoplasma pneumoniae infection causes transient depression of cell-mediated immunity [26].…”

“…Chromobacterium violaceum is characteristically resistant to penicillin, first-and second-generation cephalosporins, and has a variable susceptibility to third-generation cephalosporins, carbapenems and aminoglycosides, resulting from the presence of numerous ORFs associated with various drug resistance mechanisms in particular beta-lactam and multidrug resistance genes [25,26]. It is hypothesized that these genes are essential for Chromobacterium violaceum survival in competing with other bacteria in different ecosystems, because isolates from environment have demonstrated a higher level of antibiotic resistance than laboratory reference strains [27,28].…”

Mycoplasma Pneumoniae - Stimulation of Lymphocytes Obtained From Adenoid Vegetations and Blood in Children With and Without Serological Evidence of Mycoplasma Pneumoniae Infection

“…Antigen preparation. M.pneumoniae was cultured, washed and sonicated as previously described (15). This non-viable preparation had a protein concentration of 0.5 g/litre.…”

SPECIFICITY OF LYMPHOCYTE STIMULATION IN VITRO BY MICROBIAL PREPARATIONS: IMPORTANCE OF ANTIGEN CONCENTRATION

“…2) Mycoplasma pneumoniae was cultured, washed and sonicated as previously described (8). The preparation was employed in 7 concentrations with 3-fold dilution steps; 20 pl were added to the cultures.…”