<i>In vitro</i> three‐dimensional development of mouse molar tooth germs in a rotary cell culture system

Sun, Liang; Yang, Chao; Yang, Ge; Yu, Man; Guo, Weihua; Tian, Weidong

doi:10.1111/ipd.12057

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…Tooth germs can develop in standard tissue culture conditions for 7-8 days, but eventually become flattened and disorganized. When using 3D culture systems, this period can be slightly extended (Sun et al, 2014). Different transplantation methods have also been adopted to recapitulate the normal developmental conditions for the tooth germ where revitalisation/revascularisation can occur (Yokohama-Tamaki et al, 2006).…”

Section: Nerve -Organogenesis Interplaymentioning

confidence: 99%

Parasympathetic neurons originate from nerve-associated peripheral glial progenitors

Dyachuk

Furlan

Shahidi

et al. 2014

Science

204

182

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The peripheral autonomic nervous system reaches far throughout the body and includes neurons of diverse functions, such as sympathetic and parasympathetic. We show that the parasympathetic system in mice--including trunk ganglia and the cranial ciliary, pterygopalatine, lingual, submandibular, and otic ganglia--arise from glial cells in nerves, not neural crest cells. The parasympathetic fate is induced in nerve-associated Schwann cell precursors at distal peripheral sites. We used multicolor Cre-reporter lineage tracing to show that most of these neurons arise from bi-potent progenitors that generate both glia and neurons. This nerve origin places cellular elements for generating parasympathetic neurons in diverse tissues and organs, which may enable wiring of the developing parasympathetic nervous system.

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Section: Nerve -Organogenesis Interplaymentioning

confidence: 99%

Parasympathetic neurons originate from nerve-associated peripheral glial progenitors

Dyachuk

Furlan

Shahidi

et al. 2014

Science

204

182

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“…salivary glands (Nedvetsky et al, 2014) is still unresolved. Tooth explants that are removed grow without nerve fibers in vitro (Lumsden and Buchanan, 1986; Sun et al, 2014), but it cannot be excluded that nerves influence the ectomesenchyme or dental epithelium earlier, when teeth actually are induced. To experimentally address this in the mouse, however, would require sophisticated nerve ablation experiments that as yet have not been performed.…”

Section: Cell Types Related To Innervation and Vascular Systemmentioning

confidence: 99%

Heterogeneity and Developmental Connections between Cell Types Inhabiting Teeth

2017

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Every tissue is composed of multiple cell types that are developmentally, evolutionary and functionally integrated into the unit we call an organ. Teeth, our organs for biting and mastication, are complex and made of many different cell types connected or disconnected in terms of their ontogeny. In general, epithelial and mesenchymal compartments represent the major framework of tooth formation. Thus, they give rise to the two most important matrix–producing populations: ameloblasts generating enamel and odontoblasts producing dentin. However, the real picture is far from this quite simplified view. Diverse pulp cells, the immune system, the vascular system, the innervation and cells organizing the dental follicle all interact, and jointly participate in transforming lifeless matrix into a functional organ that can sense and protect itself. Here we outline the heterogeneity of cell types that inhabit the tooth, and also provide a life history of the major populations. The mouse model system has been indispensable not only for the studies of cell lineages and heterogeneity, but also for the investigation of dental stem cells and tooth patterning during development. Finally, we briefly discuss the evolutionary aspects of cell type diversity and dental tissue integration.

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“…Tooth germs can develop in standard tissue culture conditions for 7–8 days, but eventually become flattened and disorganized. When using 3D culture systems, this period can be slightly extended (Sun et al, 2014 ). However, tooth explant cultures have been performed with tissues that may already be committed to a dental fate, and it cannot be altogether excluded that the actual initial tooth-determining signals to epithelium and mesenchyme are of neural or glial origin.…”

Section: Nerve-organogenesis Interplay In the Cranial Compartmentmentioning

confidence: 99%

The Nervous System Orchestrates and Integrates Craniofacial Development: A Review

Adameyko

Fried

2016

Front. Physiol.

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Development of a head is a dazzlingly complex process: a number of distinct cellular sources including cranial ecto- and endoderm, mesoderm and neural crest contribute to facial and other structures. In the head, an extremely fine-tuned developmental coordination of CNS, peripheral neural components, sensory organs and a musculo-skeletal apparatus occurs, which provides protection and functional integration. The face can to a large extent be considered as an assembly of sensory systems encased and functionally fused with appendages represented by jaws. Here we review how the developing brain, neurogenic placodes and peripheral nerves influence the morphogenesis of surrounding tissues as a part of various general integrative processes in the head. The mechanisms of this impact, as we understand it now, span from the targeted release of the morphogens necessary for shaping to providing a niche for cellular sources required in later development. In this review we also discuss the most recent findings and ideas related to how peripheral nerves and nerve-associated cells contribute to craniofacial development, including teeth, during the post- neural crest period and potentially in regeneration.

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In vitro three‐dimensional development of mouse molar tooth germs in a rotary cell culture system

Abstract: Use of RCCS enhanced the development of tooth germs and allowed the tooth germs to maintain their spatial morphology. These results indicate that RCCS may be an effective culture system to investigate the mechanism of tooth development.

Cited by 5 publications

References 27 publications

Parasympathetic neurons originate from nerve-associated peripheral glial progenitors

Parasympathetic neurons originate from nerve-associated peripheral glial progenitors

Heterogeneity and Developmental Connections between Cell Types Inhabiting Teeth

The Nervous System Orchestrates and Integrates Craniofacial Development: A Review

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