<i>In vivo</i>and<i>in vitro</i>effect of bendiocarb on rabbit testicular structure and spermatozoa motility

Kročková, Jiřina; Massányi, Péter; Toman, R.; Danko, Ján; Roychoudhury, Shubhadeep

doi:10.1080/10934529.2012.672136

Cited by 15 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Motility was assessed at six time intervals: 0, 1, 2, 3, 4, and 5 h after spermatozoa collection and experiments were realized in six repetitions. The samples were evaluated doi: 10.17221/79/2017-CJAS using the CASA system (Computer Assisted Semen Analyser) with the Sperm Vision ® program (Minitube, Germany) equipped with a microscope Olympus BX 51 (Olympus Corp., Japan) to assess the spermatozoa motility (Massanyi et al 2008;Krockova et al 2012;Slanina et al 2015a). Each sample (10 μl) was placed into Makler Counting Chamber ® (depth 10 μm) (Sefi Medical Instruments Ltd., Germany).…”

Section: Methodsmentioning

confidence: 99%

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Slanina¹,

Miškeje²,

Tirpák³

et al. 2018

Czech J. Anim. Sci.

View full text Add to dashboard Cite

Slanina T., Miškeje M., Tirpák F., Błaszczyk M., Stawarz R., Massányi P. (2018): Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility. Czech J. Anim. Sci., 63, 127-135.The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine -from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision ® and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87-9.90%) if compared to 5°C (2.12-4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Slanina¹,

Miškeje²,

Tirpák³

et al. 2018

Czech J. Anim. Sci.

View full text Add to dashboard Cite

show abstract

“…In vitro studies have clearly shown that, whether determined by a computer-assisted sperm analyzer (CASA) system or simple phase-contrast microscopy, the pirethroids deltamethrin, cypermethrin and fenvalerate, as well as the organophosphates dimethoate, malathion and diazinon and the herbicides alachlor, bendiocarb, fenoxaprop-ethyl, and atrazine, can significantly reduce such parameter in humans, rodents, rabbit and boar (Table 1; Betancourt et al 2006, Grizard et al 2007, Abdallah et al 2010, Yuan et al 2010, Krockova et al 2012 followed by an increase in cell death (Betancourt et al 2006, Grizard et al 2007, Abdallah et al 2010. In parallel, the functional status of mitochondria, which is well correlated with sperm fertilizing capability (Amaral et al 2013) and widely characterized by the determination of the mitochondrial membrane potential (MMP), is also affected by EDCs (Table 1).…”

Section: Pesticidesmentioning

confidence: 99%

The non-genomic effects of endocrine-disrupting chemicals on mammalian sperm

Tavares

Escada-Rebelo

Correia³

et al. 2016

Reproduction

View full text Add to dashboard Cite

Exposure to toxicants present in the environment, especially the so-called endocrine-disrupting chemicals (EDCs), has been associated with decreased sperm quality and increased anomalies in male reproductive organs over the past decades. Both human and animal populations are continuously exposed to ubiquitous synthetic and natural-occurring EDCs through diet, dermal contact and/or inhalation, therefore potentially compromising male reproductive health. Although the effects of EDC are likely induced via multiple genomic-based pathways, their non-genomic effects may also be relevant. Furthermore, spermatozoa are transcriptionally inactive cells that can come in direct contact with EDCs in reproductive fluids and secretions and are therefore a good model to address non-genomic effects. This review thus focuses on the non-genomic effects of several important EDCs relevant to mammalian exposure. Notably, EDCs were found to interfere with pre-existing pathways inducing a panoply of deleterious effects to sperm function that included altered intracellular Ca 2C oscillations, induction of oxidative stress, mitochondrial dysfunction, increased DNA damage and decreased sperm motility and viability, among others, potentially jeopardizing male fertility. Although many studies have used non-environmentally relevant concentrations of only one compound for mechanistic studies, it is important to remember that mammals are not exposed to one, but rather to a multitude of environmental EDCs, and synergistic effects may occur. Furthermore, some effects have been detected with single compounds at environmentally relevant concentrations.Reproduction (2016) 151 R1-R13

show abstract

“…Each of the prepared samples were evaluated using a CASA system -Sperm Vision® program (Minitub, Tiefenbach, Germany) equipped with a microscope (Olympus BX 51, Japan) to assess the spermatozoa motility (Massányi et al 2008;Kročková et al 2012). Each sample was placed into Makler Counting Chamber® (depth 10 µm, Sefi-Medical Instruments, Germany).…”

Section: Motility Analysismentioning

confidence: 99%

The effect of diluent, temperature and age on turkey spermatozoa motilityin vitro

Slanina

Petrovičová

Miškeje

et al. 2014

Journal of Applied Animal Research

Self Cite

View full text Add to dashboard Cite

The aim of this study was to evaluate the turkey spermatozoa motility in in vitro conditions and to prove the effect of different conditions of incubation -diluents, temperature and age of birds. Spermatozoa were obtained from adult turkey's line of Big 6, and spermatozoa motility parameters were evaluated using a computer-assisted semen analyzer (CASA) system. Significant decrease of spermatozoa motility at laboratory temperature (22°C) was detected from time 0 (94.15%) till 180 minutes of incubation (53.91%). At the cool media incubation (5°C), this difference was lower (95.41 and 78.86%, respectively), and the differences were significant from 30 minutes of incubation till 180 minutes. Progressive spermatozoa motility replicated the tendency of total spermatozoa motility. When the physiological solution to commercial diluent at 5°C was compared, the spermatozoa motility and progressive motility in both groups were very consistent for 90 minutes of incubation. Subsequently, significantly higher spermatozoa motility was detected at time periods 120, 150 and 180 minutes of incubation in commercial diluent. Motility was also higher in this group after 24 hours. Influence of age on spermatozoa motility parameters was analysed at 22°C at the time 0 and after 30 minutes of incubation. Analysis of spermatozoa motility as well as progressive spermatozoa motility proved higher values in Group A (aged 35-42 weeks) compared to Group B (aged 63-73 weeks). These results clearly suggest that low temperature and commercial diluents maintain motility parameters during longer time periods and the increasing age of birds has negative impact on motility parameters.

show abstract

In vivoandin vitroeffect of bendiocarb on rabbit testicular structure and spermatozoa motility

Cited by 15 publications

References 13 publications

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

The non-genomic effects of endocrine-disrupting chemicals on mammalian sperm

The effect of diluent, temperature and age on turkey spermatozoa motilityin vitro

Contact Info

Product

Resources

About