In vivosimultaneous multispectral fluorescence imaging with spectral multiplexed volume holographic imaging system

Abstract

A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in the scene. The implementation and performance of the simultaneous multispectral imaging system are presented. Further, the system’s capability in simultaneously obtaining multispectral fluorescence measurements is demonstrated with in vivo experiments on a mouse. The demonstrated imaging system has the potential to obtain multispectral images fluorescence simultaneously.

Multiplex fluorescence imaging‐guided programmed delivery of doxorubicin and curcumin from a nanoparticles/hydrogel system for synergistic chemotherapy

“…[23][24][25] Multiplex fluorescence imaging could separate and monitor each signal of multiple fluorescent components by the advanced multispectral unmixing technique. 26,27 In our previous studies, dual fluorescence imaging successfully guided the drug delivery including the drug distribution and carrier location by fluorescence signal separation. [28][29][30] Recently, we have tried to investigate the delivery of dual drugs by multiplex fluorescence imaging using fluorescence reagents as drug models.…”

“…Compared to them, fluorescence imaging offers unparalleled advantages in tracking multiple fluorescent signals simultaneously 23–25 . Multiplex fluorescence imaging could separate and monitor each signal of multiple fluorescent components by the advanced multispectral unmixing technique 26,27 . In our previous studies, dual fluorescence imaging successfully guided the drug delivery including the drug distribution and carrier location by fluorescence signal separation 28–30 .…”

Reduction of blurring in broadband volume holographic imaging using a deconvolution method

“…Broadband illumination imaging, such as white light and fluorescence, is widely used in many biomedical applications. When the imaging object emits fluorescence or scatters broadband light, the contrast of the image acquired with VHI system deteriorates in the horizontal direction [10], which can have noticeable effect on the diagnosis of disease. Therefore, a better understanding of the imaging performance of volume holograms under broadband illumination is necessary to optimize the construction of VHI system for various biomedical applications.…”