<i>Lymphatic Tissue Engineering</i>

Hitchcock, Thomas; Niklason, Laura E.

doi:10.1196/annals.1413.004

Cited by 28 publications

(16 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our model also utilises more aspects of the entire lymphatic drainage (e.g. the regular deformations of the surrounding matrix to lymphatic capillaries) than previous models of the primary lymphatic valve system [13,18,21,38,42].…”

Section: Discussionmentioning

confidence: 99%

A Model for Interstitial Drainage Through a Sliding Lymphatic Valve

2015

View full text Add to dashboard Cite

This study investigates fluid flow and elastic deformation in tissues that are drained by the primary lymphatic system. A model is formulated based on the Rossi hypothesis that states that the primary lymphatic valves, which are formed by overlapping endothelial cells around the circumferential lining of lymphatic capillaries, open in response to swelling of the surrounding tissue. Tissue deformation and interstitial fluid flow through the tissue are treated using the Biot equations of poroelasticity and, the fluid flux, (into the interstitium) across the walls of the blood capillaries, is assumed to be linearly related to the pressure difference across the walls via a constant of proportionality (the vascular permeability). The resulting model is solved in a periodic domain containing one blood capillary and one lymphatic capillary starting from a configuration in which the tissue is undeformed. On imposition of a constant pressure difference between blood and lymphatic capillaries the solutions are found to settle to a steady state. Given that the magnitude of pressure fluctuations in the lymphatic system is much smaller than this pressure difference between blood and lymph it is postulated that the resulting steady state solution gives a good representation of the state of the tissue under physiological conditions. The effects of changes to the Young's modulus of the tissue, the blood-lymphatic pressure difference, vascular permeability and valve dimensions on the steady state are investigated and discussed in terms of their effects on oedema in the context of age-and pregnancy-related changes to the body.

show abstract

Section: Discussionmentioning

confidence: 99%

A Model for Interstitial Drainage Through a Sliding Lymphatic Valve

2015

View full text Add to dashboard Cite

show abstract

“…The LECs embryologically originate from out pouching of blood endothelial cells from cardinal vein, and they both share close structural similarities. About 300 genes are expressed differentially between blood endothelial cells and LECs, which includes LEC-specific genes (lymphatic vessel hyaluronan receptor 1, vascular endothelial growth factor receptor 3, prox1, podoplanin, and β-chemokine receptor D6), integrins, cadherins, proinflammatory cytokines, and chemokines [9]. …”

Section: Tissue Engineering Of Lymphatic Graftmentioning

confidence: 99%

Tissue-engineered lymphatic graft for the treatment of lymphedema

Kanapathy

Patel

Kalaskar

et al. 2014

Journal of Surgical Research

View full text Add to dashboard Cite

Background Lymphedema is a chronic debilitating condition and curative treatment is yet to be found. Tissue engineering approach, which combines cellular components, scaffold, and molecular signals hold great potential in the treatment of secondary lymphedema with the advent of lymphatic graft to reconstruct damaged collecting lymphatic vessel. This review highlights the ideal characteristics of lymphatic graft, the limitation and challenges faced, and the approaches in developing tissue-engineered lymphatic graft. Methods Literature on tissue engineering of lymphatic system and lymphatic tissue biology was reviewed. Results The prime challenge in the design and manufacturing of this graft is producing endothelialized conduit with intraluminal valves. Suitable scaffold material is needed to ensure stability and functionality of the construct. Endothelialization of the construct can be enhanced via biofunctionalization and nanotopography, which mimics extracellular matrix. Nanocomposite polymers with improved performance over existing biomaterials are likely to benefit the development of lymphatic graft. Conclusions With the in-depth understanding of tissue engineering, nanotechnology, and improved knowledge on the biology of lymphatic regeneration, the aspiration to develop successful lymphatic graft is well achievable.

show abstract

“…Engineered three-dimensional (3D) microvascular networks are also expected to find widespread clinical use as a treatment to reverse tissue ischemia caused by disease or injury (Hitchcock and Niklason, 2008). In addition, there is a broad need for a variety of vascularized 3D models of physiological systems that can better predict efficacy, safety, bioavailability and toxicology outcomes for candidate therapeutics (Lin and Chang, 2008).…”

Section: Introductionmentioning

confidence: 99%

Ultrasound patterning technologies for studying vascular morphogenesis in 3D

Comeau

Hocking

Dalecki

2016

Journal of Cell Science

View full text Add to dashboard Cite

Investigations in this report demonstrate the versatility of ultrasoundbased patterning and imaging technologies for studying determinants of vascular morphogenesis in 3D environments. Forces associated with ultrasound standing wave fields (USWFs) were employed to non-invasively and volumetrically pattern endothelial cells within 3D collagen hydrogels. Patterned hydrogels were composed of parallel bands of endothelial cells located at nodal regions of the USWF and spaced at intervals equal to one half wavelength of the incident sound field. Acoustic parameters were adjusted to vary the spatial dimensions of the endothelial bands, and effects on microvessel morphogenesis were analyzed. High-frequency ultrasound imaging techniques were used to image and quantify the spacing, width and density of initial planar cell bands. Analysis of resultant microvessel networks showed that vessel width, orientation, density and branching activity were strongly influenced by the initial 3D organization of planar bands and, hence, could be controlled by acoustic parameters used for patterning. In summary, integration of USWF-patterning and high-frequency ultrasound imaging tools enabled fabrication of vascular constructs with defined microvessel size and orientation, providing insight into how spatial cues in 3D influence vascular morphogenesis.

show abstract

Lymphatic Tissue Engineering

Cited by 28 publications

References 30 publications

A Model for Interstitial Drainage Through a Sliding Lymphatic Valve

A Model for Interstitial Drainage Through a Sliding Lymphatic Valve

Tissue-engineered lymphatic graft for the treatment of lymphedema

Ultrasound patterning technologies for studying vascular morphogenesis in 3D

Contact Info

Product

Resources

About