<i>MALE GAMETOPHYTE DEFECTIVE 2</i>, Encoding a Sialyltransferase‐like Protein, is Required for Normal Pollen Germination and Pollen Tube Growth in <i>Arabidopsis</i>

Deng, Yi Zhen; Wang, Wei; Li, Wenqing; Xia, Chuan; Liao, Hong-Ze; Zhang, Xue-Qin; Ye, De

doi:10.1111/j.1744-7909.2010.00963.x

Cited by 34 publications

(36 citation statements)

References 47 publications

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“…S5A at JXB online). To confirm this result, the GFP–CSLD1/4 plants were crossed with transgenic lines expressing the Golgi marker protein RPA (Song et al , 2006; Deng et al , 2010) with a C-terminal-fused red fluorescent tag, DsRed2, under the control of the pollen-specific LAT52 promoter (RPA–DsRed2). In mature pollen grains, as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Arabidopsis CSLD1 and CSLD4 are required for cellulose deposition and normal growth of pollen tubes

Wang

Chen

et al. 2011

Journal of Experimental Botany

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The cell wall is important for pollen tube growth, but little is known about the molecular mechanism that controls cell wall deposition in pollen tubes. Here, the functional characterization of the pollen-expressed Arabidopsis cellulose synthase-like D genes CSLD1 and CSLD4 that are required for pollen tube growth is reported. Both CSLD1 and CSLD4 are highly expressed in mature pollen grains and pollen tubes. The CSLD1 and CSLD4 proteins are located in the Golgi apparatus and transported to the plasma membrane of the tip region of growing pollen tubes, where cellulose is actively synthesized. Mutations in CSLD1 and CSLD4 caused a significant reduction in cellulose deposition in the pollen tube wall and a remarkable disorganization of the pollen tube wall layers, which disrupted the genetic transmission of the male gametophyte. In csld1 and csld4 single mutants and in the csld1 csld4 double mutant, all the mutant pollen tubes exhibited similar phenotypes: the pollen tubes grew extremely abnormally both in vitro and in vivo, which indicates that CSLD1 and CSLD4 are not functionally redundant. Taken together, these results suggest that CSLD1 and CSLD4 play important roles in pollen tube growth, probably through participation in cellulose synthesis of the pollen tube wall.

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Section: Resultsmentioning

confidence: 99%

Arabidopsis CSLD1 and CSLD4 are required for cellulose deposition and normal growth of pollen tubes

Wang

Chen

et al. 2011

Journal of Experimental Botany

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“…These include an Arabidopsis thaliana homolog (AT1G08660) of mgv1a005647m, a glycosyl transferase for which mutants are known to show reduced pollen tube germination and growth rates [72], along with an A. thaliana homolog (AT1G14420) of mgv1a006379, a pectate lyase-like gene first characterized in tomato (LAT59) and notable for studies of it's pollen-specific promoter element [73]. In addition, they include an M. guttatus homolog (mgv1a000017m) of A. thaliana KINGY POLLEN (KIP; AT5G649680) [74].…”

Section: Discussionmentioning

confidence: 99%

Selection on Plant Male Function Genes Identifies Candidates for Reproductive Isolation of Yellow Monkeyflowers

et al. 2013

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Understanding the genetic basis of reproductive isolation promises insight into speciation and the origins of biological diversity. While progress has been made in identifying genes underlying barriers to reproduction that function after fertilization (post-zygotic isolation), we know much less about earlier acting pre-zygotic barriers. Of particular interest are barriers involved in mating and fertilization that can evolve extremely rapidly under sexual selection, suggesting they may play a prominent role in the initial stages of reproductive isolation. A significant challenge to the field of speciation genetics is developing new approaches for identification of candidate genes underlying these barriers, particularly among non-traditional model systems. We employ powerful proteomic and genomic strategies to study the genetic basis of conspecific pollen precedence, an important component of pre-zygotic reproductive isolation among yellow monkeyflowers (Mimulus spp.) resulting from male pollen competition. We use isotopic labeling in combination with shotgun proteomics to identify more than 2,000 male function (pollen tube) proteins within maternal reproductive structures (styles) of M. guttatus flowers where pollen competition occurs. We then sequence array-captured pollen tube exomes from a large outcrossing population of M. guttatus, and identify those genes with evidence of selective sweeps or balancing selection consistent with their role in pollen competition. We also test for evidence of positive selection on these genes more broadly across yellow monkeyflowers, because a signal of adaptive divergence is a common feature of genes causing reproductive isolation. Together the molecular evolution studies identify 159 pollen tube proteins that are candidate genes for conspecific pollen precedence. Our work demonstrates how powerful proteomic and genomic tools can be readily adapted to non-traditional model systems, allowing for genome-wide screens towards the goal of identifying the molecular basis of genetically complex traits.

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“…Many pollen germination and pollen tube growth mutants have been identified recently. The male gametophyte defective2 (mgp2) mutant shows inhibited pollen germination (Deng et al, 2010). FIMBRIN5 (FIM5) is needed in actin cytoskeleton organization in pollen grains and pollen tubes.…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis AtVPS15 Plays Essential Roles in Pollen Germination Possibly by Interacting with AtVPS34

Wang

Zhang

Xing

et al. 2012

Journal of Genetics and Genomics

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MALE GAMETOPHYTE DEFECTIVE 2, Encoding a Sialyltransferase‐like Protein, is Required for Normal Pollen Germination and Pollen Tube Growth in Arabidopsis

Cited by 34 publications

References 47 publications

Arabidopsis CSLD1 and CSLD4 are required for cellulose deposition and normal growth of pollen tubes

Arabidopsis CSLD1 and CSLD4 are required for cellulose deposition and normal growth of pollen tubes

Selection on Plant Male Function Genes Identifies Candidates for Reproductive Isolation of Yellow Monkeyflowers

Arabidopsis AtVPS15 Plays Essential Roles in Pollen Germination Possibly by Interacting with AtVPS34

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