<i>Marinobacter</i>: A case study in bioelectrochemical chassis evaluation

Bird, Lina J.; Mickol, R. L.; Eddie, Brian J.; Thakur, Meghna; Yates, Matthew D.; Glaven, Sarah M.

doi:10.1111/1751-7915.14170

Cited by 14 publications

(6 citation statements)

References 133 publications

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“…These strains acquired tolerance to these compounds through adjustments made in the phospholipid composition of cell membranes, increased robustness of the protein folding machinery, and a set of efflux pumps that extrude solvents to the external environment from the cytoplasm, periplasm and cell membranes [ 23 ]. In addition to solvent tolerance, a crucial characteristic of microbial chassis for chemical production [ 46 , 47 ] is the ability to maintain the integrity of the desired product(s). In the case of P. putida CM12-5 strain, a phenylalanine producing strain catabolism of the aromatic amino acid is blocked, in addition, the strain does not metabolize t CA and styrene, compounds that can be made from L-phenylalanine [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas

García-Franco,

Godoy,

Duque

et al. 2024

Microb Cell Fact

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We are interested in converting second generation feedstocks into styrene, a valuable chemical compound, using the solvent-tolerant Pseudomonas putida DOT-T1E as a chassis. Styrene biosynthesis takes place from L-phenylalanine in two steps: firstly, L-phenylalanine is converted into trans-cinnamic acid (tCA) by PAL enzymes and secondly, a decarboxylase yields styrene. This study focuses on designing and synthesizing a functional trans-cinnamic acid decarboxylase in Pseudomonas putida. To achieve this, we utilized the “wholesale” method, involving deriving two consensus sequences from multi-alignments of homologous yeast ferulate decarboxylase FDC1 sequences with > 60% and > 50% identity, respectively. These consensus sequences were used to design Pseudomonas codon-optimized genes named psc1 and psd1 and assays were conducted to test the activity in P. putida. Our results show that the PSC1 enzyme effectively decarboxylates tCA into styrene, whilst the PSD1 enzyme does not. The optimal conditions for the PSC1 enzyme, including pH and temperature were determined. The L-phenylalanine DOT-T1E derivative Pseudomonas putida CM12-5 that overproduces L-phenylalanine was used as the host for expression of pal/psc1 genes to efficiently convert L-phenylalanine into tCA, and the aromatic carboxylic acid into styrene. The highest styrene production was achieved when the pal and psc1 genes were co-expressed as an operon in P. putida CM12-5. This construction yielded styrene production exceeding 220 mg L−1. This study serves as a successful demonstration of our strategy to tailor functional enzymes for novel host organisms, thereby broadening their metabolic capabilities. This breakthrough opens the doors to the synthesis of aromatic hydrocarbons using Pseudomonas putida as a versatile biofactory.

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Section: Discussionmentioning

confidence: 99%

Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas

García-Franco,

Godoy,

Duque

et al. 2024

Microb Cell Fact

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“…5). Previous studies demonstrated its ability to synthesize various compounds and degrade hydrocarbons in high-salinity environments (37). In addition, Marinobacter were shown to control the formation of bio lms associated with calcium levels(38).…”

Section: Discussionmentioning

confidence: 99%

Prokaryotic microbial diversity analysis and metabolic function prediction of salt lakes on the Qinghai-Tibet Plateau

Zhang,

Li,

Zhu

et al. 2023

Preprint

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The Dong Taijinar Salt Lake (DT) and Xi Taijinar Salt Lake (XT) have been widely studied as mineral-rich areas. However, little is known about the composition and distribution of the microbial communities in these two lakes. In this study, metagenomics sequencing was used to analyze the diversity and potential functions of the microbial communities in DT and XT. According to our report, the salinity of DT (332.18–358.30 g/L) was 10 times higher than that of XT (20.09–36.83 g/L). Interestingly, the dominant domain in DT was Archaea (96.16%), while that in XT was Bacteria (93.09%). The distribution of Bacteria in the DT revealed 33 phyla and 1717 genera. The dominant genus in DT was Marinobacillus, which was positively correlated with total phosphorus content. There were four main phyla and 153 genera identified in the Archaea of DT. The most abundant Archaea genera in DT were Natronomonas (24.61%) and Halorubrum (23.69%), which were mainly positively correlated with the Na+, Ca2+, and Cl− contents. Similarly, there were 33 phyla and 1906 genera of Bacteria in XT, and Loktanella was the dominant genus. The archaeal taxonomy in XT mainly included four phyla and 149 genera. Proteobacteria and Euryarchaeota were the most abundant bacterial and archaeal phyla in the two salt lakes. Analysis of the halophilic mechanisms of the microorganisms identified in these two salt lakes revealed that the Bacteria in XT preferred to synthesize compatible solutes, whereas the Archaea in DT preferred a "salt-in" adaptation strategy in salt-stressed environments.

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“…5). Previous studies demonstrated its ability to synthesize various compounds and degrade hydrocarbons in high-salinity environments (47). In addition, Marinobacter were shown to control the formation of biofilms associated with calcium levels (48).…”

Section: Discussionmentioning

confidence: 99%

Prokaryotic microbial diversity analysis and metabolic function prediction of salt lakes on the Qinghai-Tibet Plateau

Zhang

Zhu

et al. 2023

Preprint

View full text Add to dashboard Cite

The Dong Taijinar Salt Lake (DT) and Xi Taijinar Salt Lake (XT) have been widely studied as mineral-rich areas. However, little is known about the composition and distribution of the microbial communities in these two lakes. In this study, metagenomics sequencing was used to analyze the diversity and potential functions of the microbial communities in DT and XT. According to our report, the salinity of DT (332.18–358.30 g/L) was 10 times higher than that of XT (20.09–36.83 g/L). Interestingly, the dominant domain in DT wasArchaea(96.16%), while that in XT wasBacteria(93.09%). The distribution ofBacteriain the DT revealed 33 phyla and 1717 genera. The dominant genus in DT was Marinobacillus, which was positively correlated with total phosphorus content. There were four main phyla and 153 genera identified in theArchaeaof DT. The most abundantArchaeagenera in DT were Natronomonas (24.61%) and Halorubrum (23.69%), which were mainly positively correlated with the Na+, Ca2+, and Cl−contents. Similarly, there were 33 phyla and 1906 genera ofBacteriain XT, and Loktanella was the dominant genus. The archaeal taxonomy in XT mainly included four phyla and 149 genera. Proteobacteria and Euryarchaeota were the most abundant bacterial and archaeal phyla in the two salt lakes. Analysis of the halophilic mechanisms of the microorganisms identified in these two salt lakes revealed that the Bacteria in XT preferred to synthesize compatible solutes, whereas the Archaea in DT preferred a "salt-in" adaptation strategy in salt-stressed environments.

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Marinobacter: A case study in bioelectrochemical chassis evaluation

Cited by 14 publications

References 133 publications

Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas

Engineering styrene biosynthesis: designing a functional trans-cinnamic acid decarboxylase in Pseudomonas

Prokaryotic microbial diversity analysis and metabolic function prediction of salt lakes on the Qinghai-Tibet Plateau

Prokaryotic microbial diversity analysis and metabolic function prediction of salt lakes on the Qinghai-Tibet Plateau

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