2019
DOI: 10.1128/iai.00750-19
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Mycobacterium tuberculosis LipE Has a Lipase/Esterase Activity and Is Important for Intracellular Growth and In Vivo Infection

Abstract: Mycobacterium tuberculosis Rv3775 (LipE) was annotated as a putative lipase. However, its lipase activity has never been characterized, and its precise role in tuberculosis (TB) pathogenesis has not been thoroughly studied to date. We overexpressed and purified the recombinant LipE (rLipE) protein and demonstrated that LipE has a lipase/esterase activity. rLipE prefers medium-chain ester substrates, with the maximal activity on hexanoate. Its activity is the highest at 40°C and pH 9.

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Cited by 9 publications
(12 citation statements)
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“… 12 , 13 The transcription of some genes can be induced by Mtb infection. And some genes encode molecules necessary for Mtb growth and reproduction such as lipase, 14 isocitrate lyase, 15 phospholipase, 16 mycotic acid lyase, 17 free fatty acid coenzyme A (CoA), 18 and acyl-coenzyme A dehydrogenase. 19 The mutation of genes related to lipid metabolism can alleviate the symptoms of Mtb -infected cells or animals.…”
Section: Discussionmentioning
confidence: 99%
“… 12 , 13 The transcription of some genes can be induced by Mtb infection. And some genes encode molecules necessary for Mtb growth and reproduction such as lipase, 14 isocitrate lyase, 15 phospholipase, 16 mycotic acid lyase, 17 free fatty acid coenzyme A (CoA), 18 and acyl-coenzyme A dehydrogenase. 19 The mutation of genes related to lipid metabolism can alleviate the symptoms of Mtb -infected cells or animals.…”
Section: Discussionmentioning
confidence: 99%
“…Additional virulence determinants that may contribute to M.tb pathogenesis including proteases ( zmp1 , clpP1 and pepN ) (56, 57), lipases ( lipE ) (58), iron efflux pumps ( MmpL4 ) (59) and adhesins ( apa , fbpB , glnA1 , gap and pstS1 ) (60) were found upregulated in E i vs . E, as well as other genes associated with mycobacterial survival ( glpX and Rv1096 ) (61, 62) ( Suppl.…”
Section: Resultsmentioning
confidence: 99%
“…Metalloprotease Zmp-1 is involved in inflammasome inactivation and phagosome maturation arrest and is essential for intracellular survival (78, 79), and clpP1 is critical for M.tb growth (80). Similarly, LipE with lipase/esterase activity is induced under stress conditions and is essential for M.tb intracellular growth in vivo (58). Lastly, several adhesins such as apa , fbpB , glnA1 , gap, and pstS1 were upregulated in E-ALF-exposed M.tb during infection.…”
Section: Discussionmentioning
confidence: 99%
“…For example, Mycobacterium tuberculosis virulence, persistence, and drug-resistance have been linked to lipid acquisition and metabolism [43]. M. tuberculosis is predicted to possess up to 30 lipolytic enzymes [44,45], some of which contribute to virulence mechanisms such as intracellular growth, bacterial loads in mice and recovery from dormancy [46][47][48][49][50]. Pseudomonas aeruginosa lipases, LipA and LipC, influence motility and biofilm formation as well as the production of virulence factors pyoverdine and rhamnolipid [51,52].…”
Section: Plos Pathogensmentioning
confidence: 99%