<i>OsGRF4</i> controls grain shape, panicle length and seed shattering in rice

Sun, Pingyong; Zhang, Wuhan; Wang, Yihua; He, Qingfang; Fu, Shu Man; Hai, Liu; Wang, Jie; Jian-min, Wang; Yuan, Longping; Deng, Huafeng

doi:10.1111/jipb.12473

Cited by 159 publications

(119 citation statements)

References 44 publications

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“…OsGRF4 , a target of miR396, reportedly regulates a CK dehydrogenase precursor gene and controls rice grain shape (Sun et al, 2016), while TCP14 and TCP15 TFs, which mediate CK responses, were found to cause excessive proliferation of the boundaries of the Arabidopsis gynoecium replum (Takei et al, 2001). Isopentenyl adenine (iPA) and zeatin riboside (ZR) are the major CK species in maize (Takei et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Development of Incompletely Fused Carpels in Maize Ovary Revealed by miRNA, Target Gene and Phytohormone Analysis

Peng

Wang

et al. 2017

Front. Plant Sci.

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Although the molecular basis of carpel fusion in maize ovary development remains largely unknown, increasing evidence suggests a critical role of microRNAs (miRNAs). In this study, a combination of miRNA sequencing, degradome and physiological analyses was used to characterize carpel fusion development in maize ovaries showing incompletely (IFC) and completely fused carpels (CFC). A total of 162 known miRNAs distributed across 33 families were identified, of which 20 were differentially expressed. In addition, 53 miRNA candidates were identified, of which 10 were differentially expressed in the IFC and CFC ovaries. In degradome analysis, a total of 113 and 11 target genes were predicted for the known and novel miRNAs, respectively. Moreover, 24 (60%) target genes of the differentially expressed known miRNAs were found to code transcription factors, including auxin response factor (ARF), TB1-CYC-PCFs (TCP), APETALA2 (AP2), growth regulating factor (GRF), MYB, NAC, and NF-YA, all of which have been shown to play a role in carpel fusion development. Correlation analysis of these differentially expressed known miRNAs and their targets with phytohormone signals revealed significant correlations with at least one phytohormone signal, the main regulator of carpel fusion development. These results suggest that incomplete carpel fusion is partly the result of differential expression of certain miRNAs and their targets. Overall, these findings improve our knowledge of the effect of miRNA regulation on target expression, providing a useful resource for further analysis of the interactions between miRNAs, target genes and phytohormones during carpel fusion development in maize.

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Section: Discussionmentioning

confidence: 99%

Development of Incompletely Fused Carpels in Maize Ovary Revealed by miRNA, Target Gene and Phytohormone Analysis

Peng

Wang

et al. 2017

Front. Plant Sci.

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“…Grain size and shape in rice are complex genetic traits, controlled by a large number of quantitative trait loci (QTLs). A number of regulatory genes have been identified through QTL mapping, including GW2, GS 3, BG1 , GW8, GW7 and GW5 (Zuo and Li ; Sun et al ; Li et al ; Xu et al ). For example, GW2 encodes an ortholog of Arabidopsis DA2 and controls seed size, via the ubiquitin‐proteasome pathway.…”

Section: Introductionmentioning

confidence: 99%

GW5‐Like, a homolog of GW5, negatively regulates grain width, weight and salt resistance in rice

Tian

Liu

Mou

et al. 2019

JIPB

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Grain size is an important determinant of yield potential in crops. We previously demonstrated that natural mutations in the regulatory sequences of qSW5/GW5 confer grain width diversity in rice. However, the biological function of a GW5 homolog, named GW5‐Like (GW5L), remains unknown. In this study, we report on GW5L knockout mutants in Kitaake, a japonica cultivar (cv.) considered to have a weak gw5 variant allele that confers shorter and wider grains. GW5L is evenly expressed in various tissues, and its protein product is localized to the plasma membrane. Biochemical assays verified that GW5L functions in a similar fashion to GW5. It positively regulates brassinosteroid (BR) signaling through repression of the phosphorylation activity of GSK2. Genetic data show that GW5L overexpression in either Kitaake or a GW5 knockout line, Kasaorf3 (indica cv. Kasalath background), causes more slender, longer grains relative to the wild‐type. We also show that GW5L could confer salt stress resistance through an association with calmodulin protein OsCaM1‐1. These findings identify GW5L as a negative regulator of both grain size and salt stress tolerance, and provide a potential target for breeders to improve grain yield and salt stress resistance in rice.

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“…PL QTL is commonly co‐identified with heading date (Liu et al, ). Growth‐Regulating Factor 4 ( OsGRF4 ) controls synchronously PL, grain shape and seed shattering in rice by regulating two cytokinin dehydrogenase precursor genes ( CKX5 and CKX1 ) resulting in increased cytokinin level (Sun et al, ). Additionally, for the three major QTLs qPL10 , qSPP10 and qPH10 , their LOD confidence intervals mostly overlapped one another in the same interval between the markers RM1375 and RM3229 on chromosome 10, indicating that the locus in this interval might harbour a gene with pleiotropic effects on the traits of PH, PL and SPP (Kovi et al, ).…”

Section: Discussionmentioning

confidence: 99%

Identification and application of major quantitative trait loci for panicle length in rice (Oryza sativa) through single‐segment substitution lines

et al. 2019

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Panicle length (PL), an important yield‐related trait, strongly affects yield components, such as grain number, grain density and rice quality. More than 200 panicle length quantitative trait loci (PL QTLs) are identified, but only a small number are applied in rice breeding. In this study, we performed QTL analysis for PL using 42 single‐segment substitution lines (SSSLs) derived from nine donors in the genetic background of HJX74. Fourteen QTLs and five heterosis QTLs (HQTLs) for PL were recognised. Three QTLs and four HQTLs acted positively, and the other eleven QTLs and one HQTL acted negatively. By scanning the single heterozygous background region of the F2 population with large‐genetic‐effect SSSLs, we mapped PL loci qPL6‐2 and qPL7‐1 to different locations on chromosomes 6 and 7, respectively, in three consecutive years of independent trials. The genetic effects of these QTLs were further assessed. qPL6‐2 demonstrated the most positive additive effect (QTL), whereas qPL7‐1 achieved the most positive dominant effect (HQTL) for PL. These results indicated that the pyramiding of PL QTLs might increase grain yield and facilitate the application of the beneficial allele in hybrid rice breeding.

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OsGRF4 controls grain shape, panicle length and seed shattering in rice

Cited by 159 publications

References 44 publications

Development of Incompletely Fused Carpels in Maize Ovary Revealed by miRNA, Target Gene and Phytohormone Analysis

Development of Incompletely Fused Carpels in Maize Ovary Revealed by miRNA, Target Gene and Phytohormone Analysis

GW5‐Like, a homolog of GW5, negatively regulates grain width, weight and salt resistance in rice

Identification and application of major quantitative trait loci for panicle length in rice (Oryza sativa) through single‐segment substitution lines

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