<i>PALE-GREEN LEAF12</i> Encodes a Novel Pentatricopeptide Repeat Protein Required for Chloroplast Development and 16S rRNA Processing in Rice

Chen, Long; Huang, Lichao; Dai, Liping; Gao, Yi-hong; Zou, Weiwei; Lu, Xueli; Wang, Changjian; Zhang, Guangheng; Ren, Deyong; Hu, Jiang; Shen, Lan; Gao, Zhenyu; Chen, Guang; Xue, Dawei; Guo, Longbiao; Xing, Yongzhong; Qian, Qian; Zhu, Li

doi:10.1093/pcp/pcy229

Cited by 30 publications

(15 citation statements)

References 63 publications

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“…The chloroplast-localized OsWSL5 is predominantly involved in the intron splicing of rpl2 and rps12 in chloroplasts (Liu et al 2018). The chloroplast-localized OsPGL12 is mainly involved in the intron splicing of ndhA in chloroplasts (Chen et al 2019). The mitochondrion-localized OsFLO10 is mainly involved in the trans-splicing of the nad1 intron 1 in mitochondria (Wu et al 2019), and the nucleus-localized OsNPPR1 was reported to be involved in the intron splicing of a small number of nuclearlocalized genes, many of which are mitochondrionlocalized (Hao et al 2019).…”

Section: Introductionmentioning

confidence: 99%

OsSLC1 Encodes a Pentatricopeptide Repeat Protein Essential for Early Chloroplast Development and Seedling Survival

Shang

Chen

et al. 2020

Rice

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Background: The large family of pentatricopeptide repeat (PPR) proteins is widely distributed among land plants. Such proteins play vital roles in intron splicing, RNA editing, RNA processing, RNA stability and RNA translation. However, only a small number of PPR genes have been identified in rice. Results: In this study, we raised a mutant from tissue-culture-derived plants of Oryza sativa subsp. japonica 'Zhonghua 11', which exhibited a lethal chlorosis phenotype from germination to the third-leaf stage. The mutant was designated seedling-lethal chlorosis 1 (slc1). The slc1 mutant leaves showed extremely low contents of photosynthetic pigments and abnormal chloroplast development, and were severely defective in photosynthesis. Map-based cloning of OsSLC1 revealed that a single base (G) deletion was detected in the first exon of Os06g0710800 in the slc1 mutant, which caused a premature stop codon. Knockout and complementation experiments further confirmed that OsSLC1 is responsible for the seedling-lethal chlorosis phenotype in the slc1 mutant. OsSLC1 was preferentially expressed in green leaves, and encoded a chloroplast-localized PPR protein harboring 12 PPR motifs. Loss-of-function of OsSLC1 affected the intron splicing of multiple group II introns, and especially precluded the intron splicing of rps16, and resulted in significant increase in the transcript levels of 3 chloroplast ribosomal RNAs and 16 chloroplast development-related and photosynthesis-related genes, and in significant reduction in the transcript levels of 1 chloroplast ribosomal RNAs and 2 chloroplast development-related and photosynthesis-related genes. Conclusion: We characterized a novel chloroplast-localized PPR protein, OsSLC1, which plays a vital role in the intron splicing of multiple group II introns, especially the rps16 intron, and is essential for early chloroplast development and seedling survival in rice.

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Section: Introductionmentioning

confidence: 99%

OsSLC1 Encodes a Pentatricopeptide Repeat Protein Essential for Early Chloroplast Development and Seedling Survival

Shang

Chen

et al. 2020

Rice

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“…Transmission electron microscopy analysis was carried out as previously described [26]. In the two-leaf stage, the leaves of WT plants and oscfm2-1 mutants were collected and cut into 0.1 cm × 0.1 cm pieces.…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Characterization of the CRM Gene Family and Elucidating the Function of OsCFM2 in Rice

et al. 2020

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The chloroplast RNA splicing and ribosome maturation (CRM) domain-containing proteins regulate the expression of chloroplast or mitochondrial genes that influence plant growth and development. Although 14 CRM domain proteins have previously been identified in rice, there are few studies of these gene expression patterns in various tissues and under abiotic stress. In our study, we found that 14 CRM domain-containing proteins have a conservative motif1. Under salt stress, the expression levels of 14 CRM genes were downregulated. However, under drought and cold stress, the expression level of some CRM genes was increased. The analysis of gene expression patterns showed that 14 CRM genes were expressed in all tissues but especially highly expressed in leaves. In addition, we analyzed the functions of OsCFM2 and found that this protein influences chloroplast development by regulating the splicing of a group I and five group II introns. Our study provides information for the function analysis of CRM domain-containing proteins in rice.

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“…In A. thaliana, AtOTP70 and AtOTP51 are required for intron splicing of rpoC1 and ycf3-2 [16,17]. In rice, WSL4 and PGL12 participate in intron splicing of rpl2 and ndhA, respectively [18,19]. Inactivation of the PPR proteins involved in chloroplast intron splicing leads to abnormal chloroplast development, with leaves showing chlorosis or albino seedling phenotypes [20,21].…”

Section: Introductionmentioning

confidence: 99%

OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice

et al. 2020

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Background Chloroplasts play an important role in plant growth and development. The chloroplast genome contains approximately twenty group II introns that are spliced due to proteins encoded by nuclear genes. CAF2 is one of these splicing factors that has been shown to splice group IIB introns in maize and Arabidopsis thaliana. However, the research of the OsCAF2 gene in rice is very little, and the effects of OsCAF2 genes on chloroplasts development are not well characterized. Results In this study, oscaf2 mutants were obtained by editing the OsCAF2 gene in the Nipponbare variety of rice. Phenotypic analysis showed that mutations to OsCAF2 led to albino leaves at the seeding stage that eventually caused plant death, and oscaf2 mutant plants had fewer chloroplasts and damaged chloroplast structure. We speculated that OsCAF2 might participate in the splicing of group IIA and IIB introns, which differs from its orthologs in A. thaliana and maize. Through yeast two-hybrid experiments, we found that the C-terminal region of OsCAF2 interacted with OsCRS2 and formed an OsCAF2-OsCRS2 complex. In addition, the N-terminal region of OsCAF2 interacted with itself to form homodimers. Conclusion Taken together, this study improved our understanding of the OsCAF2 protein, and revealed additional information about the molecular mechanism of OsCAF2 in regulating of chloroplast development in rice.

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PALE-GREEN LEAF12 Encodes a Novel Pentatricopeptide Repeat Protein Required for Chloroplast Development and 16S rRNA Processing in Rice

Cited by 30 publications

References 63 publications

OsSLC1 Encodes a Pentatricopeptide Repeat Protein Essential for Early Chloroplast Development and Seedling Survival

OsSLC1 Encodes a Pentatricopeptide Repeat Protein Essential for Early Chloroplast Development and Seedling Survival

Characterization of the CRM Gene Family and Elucidating the Function of OsCFM2 in Rice

OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice

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