<i>PGL</i>, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice

Yang, Yaolong; Xu, Jie; Huang, Lichao; Leng, Yujia; Dai, Liping; Rao, Yuchun; Long, Chen; Wang, Yuqiong; Tu, Zhengjun; Hu, Jiang; Ren, Deyong; Zhang, Guangheng; Zhu, Li; Guo, Longbiao; Qian, Qian; Zeng, Dali

doi:10.1093/jxb/erv529

Cited by 119 publications

(101 citation statements)

References 51 publications

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“…The chlorophyll content was determined according to the method previously described (Yang et al, 2016). Net photosynthesis rate was measured using a Walz GFS-3000 (Eichenring).…”

Section: Chlorophyll Content and Net Photosynthesis Ratementioning

confidence: 99%

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

et al. 2017

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To better understand the molecular mechanisms behind plant growth and leaf senescence in monocot plants, we identified a mutant exhibiting dwarfism and an early-senescence leaf phenotype, termed dwarf and early-senescence leaf1 (del1). Histological analysis showed that the abnormal growth was caused by a reduction in cell number. Further investigation revealed that the decline in cell number in del1 was affected by the cell cycle. Physiological analysis, transmission electron microscopy, and TUNEL assays showed that leaf senescence was triggered by the accumulation of reactive oxygen species. The DEL1 gene was cloned using a map-based approach. It was shown to encode a pectate lyase (PEL) precursor that contains a PelC domain. DEL1 contains all the conserved residues of PEL and has strong similarity with plant PelC. DEL1 is expressed in all tissues but predominantly in elongating tissues. Functional analysis revealed that mutation of DEL1 decreased the total PEL enzymatic activity, increased the degree of methylesterified homogalacturonan, and altered the cell wall composition and structure. In addition, transcriptome assay revealed that a set of cell wall function-and senescence-related gene expression was altered in del1 plants. Our research indicates that DEL1 is involved in both the maintenance of normal cell division and the induction of leaf senescence. These findings reveal a new molecular mechanism for plant growth and leaf senescence mediated by PECTATE LYASE-LIKE genes.

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“…The chlorophyll content was determined according to the method previously described (Yang et al, 2016). Net photosynthesis rate was measured using a Walz GFS-3000 (Eichenring).…”

Section: Chlorophyll Content and Net Photosynthesis Ratementioning

confidence: 99%

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

et al. 2017

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“…Mutations to any of these genes may reduce or even eliminate chlorophyll biosynthesis, leading to leaf color changes (Liu et al, 2007, 2016; Yoo et al, 2009; Tian et al, 2013; Wang et al, 2014; Zhang et al, 2015; Yang et al, 2016). Through gene mapping and additional studies, the mutant gene responsible for the xantha marker trait of HYB was identified as a mutated OsGUN4 ( genomes uncoupled 4 ) (Chi et al, 2010; Li et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

The xantha Marker Trait Is Associated with Altered Tetrapyrrole Biosynthesis and Deregulated Transcription of PhANGs in Rice

Jiang

Liu

et al. 2017

Front. Plant Sci.

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The xantha marker trait, which is controlled by a down-regulating epi-mutation of OsGUN4, has been applied to the production of hybrid rice. However, the molecular basis for the ability of xantha mutants to attain high photosynthetic capacity even with decreased chlorophyll contents has not been characterized. In the present study, we observed that the total chlorophyll content of the xantha mutant was only 27.2% of that of the wild-type (WT) plants. However, the xantha mutant still accumulated 59.9% of the WT δ-aminolevulinic acid content, 72.8% of the WT Mg-protoporphyrin IX content, and 63.0% of the WT protochlorophyllide a content. Additionally, the protoporphyrin IX and heme contents in the mutant increased to 155.0 and 160.0%, respectively, of the WT levels. A search for homologs resulted in the identification of 124 rice genes involved in tetrapyrrole biosynthesis and photosynthesis. With the exception of OsGUN4, OsHO-1, and OsHO-2, the expression levels of the genes involved in tetrapyrrole biosynthesis were significantly higher in the xantha mutant than in the WT plants, as were all 72 photosynthesis-associated nuclear genes. In contrast, there were no differences between the xantha mutant and WT plants regarding the expression of all 22 photosynthesis-associated chloroplast genes. Furthermore, the abundance of 1O2 and the expression levels of 1O2-related genes were lower in the xantha mutant than in the WT plants, indicating 1O2-mediated retrograde signaling was repressed in the mutant plants. These results suggested that the abundance of protoporphyrin IX used for chlorophyll synthesis decreased in the mutant, which ultimately decreased the amount of chlorophyll in the xantha mutant. Additionally, the up-regulated expression of photosynthesis-associated nuclear genes enabled the mutant to attain a high photosynthetic capacity. Our findings confirm that OsGUN4 plays an important role in tetrapyrrole biosynthesis and photosynthesis in rice. GUN4, chlorophyll synthesis pathways, and photosynthetic activities are highly conserved in plants and hence, novel traits (e.g., xantha marker trait) may be generated in other cereal crops by modifying the GUN4 gene.

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“…They were soaked in 2.5 mg ml −1 trypan blue solution at room temperature for 1 hr and then decolored by soaking in lactophenol/ethanol solution. ROS level in the leaves was detected by nitro blue tetrazolium (NBT) and diamino benzidine (DAB) staining as previously described (Yang et al, ; Wang, He, et al, ).…”

Section: Methodsmentioning

confidence: 99%

Solanum lycopersicum microRNA1916 targets multiple target genes and negatively regulates the immune response in tomato

Chen

Meng

et al. 2018

Plant Cell & Environment

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MicroRNA1916 (miR1916) is one of the nonconserved miRNAs that respond to various stresses in plants, but little has been known at present about its mechanisms in biotic stresses. In this study, the expression of Solanum lycopersicum (sly)‐miR1916 in tomato was found to be down‐regulated after infection with Phytophthora infestans or Botrytis cinerea. Tomato plants that overexpressed sly‐miR1916 displayed significant enhancement in susceptibility to P. infestans and B. cinerea infection, as well as increased tendency to produce reactive oxygen species. Silencing of sly‐miR1916 by short tandem target mimic and artificial microRNA strategies caused the tomato plants to become more tolerant to adverse conditions. In addition, lower sly‐miR1916 expression could up‐regulate the expression of strictosidine synthase (STR‐2), UDP‐glycosyltransferases (UGTs), late blight resistance protein homolog R1B‐16, disease resistance protein RPP13‐like, and MYB transcription factor (MYB12), which ultimately resulted in the accumulation of α‐tomatine and anthocyanins via STR‐2, UGT, and MYB12. Furthermore, ectopic expression of sly‐miR1916/STR‐2 significantly changed the tolerance of tobacco to B. cinerea. Taken together, the results demonstrated that sly‐miR1916 might regulate the expression of STR‐2, UGT, and MYB12 in tomato plant, conferring sensitivity to biotic stress via modulating α‐tomatine and anthocyanins.

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PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice

Abstract: Highlight PGL encodes CAO1 in rice, which is essential for Chl b synthesis and affects Chl synthesis and degradation. PGL also impacts leaf senescence and indirectly affects grain yield and quality.

Cited by 119 publications

References 51 publications

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence

The xantha Marker Trait Is Associated with Altered Tetrapyrrole Biosynthesis and Deregulated Transcription of PhANGs in Rice

Solanum lycopersicum microRNA1916 targets multiple target genes and negatively regulates the immune response in tomato

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