<i>Plasmodium vivax</i> Strains Use Alternative Pathways for Invasion

Kanjee, Usheer; Grüring, Christof; Babar, Prasad H.; Meyers, Anosha; Dash, Rashmi; Pereira, Ligia; Mascarenhas, Anjali; Chaand, Mudit; Rangel, Gabriel W.; Clark, Martha A.; Chéry, Lisly; Gomes, Edwin; Rathod, Pradipsinh K.; Duraisingh, Manoj T.

doi:10.1093/infdis/jiaa592

Cited by 25 publications

(44 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies of various culture conditions in red cells have revealed switching mechanisms in mature RBCs invasion by P. falciparum (Dolan et al, 1990). Recently, by blocking reticulocytes' DARC and TfR1 receptors in short-term cultures of P. vivax isolates, a significant variation in receptor usage was observed, suggesting that P. vivax may use alternative invasion pathways (Kanjee et al, 2020). Immature reticulocytes clearly represent the right cell to search for these alternative pathways' mediators.…”

Section: Young Reticulocytes: the Keystone For P Vivax Infection Of The Duffy Negative African Population?mentioning

confidence: 99%

Home Sweet Home: Plasmodium vivax-Infected Reticulocytes—The Younger the Better?

Thomson-Luque

Bautista

2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

After a century of constant failure to produce an in vitro culture of the most widespread human malaria parasite Plasmodium vivax, recent advances have highlighted the difficulties to provide this parasite with a healthy host cell to invade, develop, and multiply under in vitro conditions. The actual level of understanding of the heterogeneous populations of cells—framed under the name ‘reticulocytes’—and, importantly, their adequate in vitro progression from very immature reticulocytes to normocytes (mature erythrocytes) is far from complete. The volatility of its individual stability may suggest the reticulocyte as a delusory cell, particularly to be used for stable culture purposes. Yet, the recent relevance gained by a specific subset of highly immature reticulocytes has brought some hope. Very immature reticulocytes are characterized by a peculiar membrane harboring a plethora of molecules potentially involved in P. vivax invasion and by an intracellular complexity dynamically changing upon its quick maturation into normocytes. We analyze the potentialities offered by this youngest reticulocyte subsets as an ideal in vitro host cell for P. vivax.

show abstract

Section: Young Reticulocytes: the Keystone For P Vivax Infection Of The Duffy Negative African Population?mentioning

confidence: 99%

Home Sweet Home: Plasmodium vivax-Infected Reticulocytes—The Younger the Better?

Thomson-Luque

Bautista

2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Recently, an additional essential P. vivax invasion pathway has been identified. P. vivax reticulocyte-binding protein 2b (PvRBP2b) binds to human reticulocytes through an alternative pathway involving human transferrin receptor [77], perhaps expanding the available vaccine targets against P. vivax.…”

Section: Plasmodium Chimeras: Developing Models For the Nonculturable...mentioning

confidence: 99%

Leveraging genome editing to functionally evaluate Plasmodium diversity

Thiam

Mangou

et al. 2022

Trends in Parasitology

View full text Add to dashboard Cite

“…Intracellular pathogens frequently exploit receptor-ligand interactions to recognise and invade the appropriate cell types [9][10][11][12] . For extracellular parasites such as Trypanosoma brucei spp., causative agents of human and animal trypanosomiasis, it is not known what drives them to move from one host tissue to another.…”

Section: Introductionmentioning

confidence: 99%

Cyclic AMP signalling and glucose metabolism mediate pH taxis by African trypanosomes

Shaw

Knuesel

Abbuehl

et al. 2021

Preprint

View full text Add to dashboard Cite

More than a decade ago it was proposed that the collective migration of African trypanosomes on semi-solid surfaces could be explained by a combination of migration factors and repellents released by the parasites, but the identity of these molecules was unknown. Here we show that procyclic (insect midgut) forms acidify their environment as a consequence of glucose metabolism, generating pH gradients by diffusion. Early and late procyclic forms exhibit self-organising properties on surfaces. Both forms are attracted to alkali, but while early procyclic forms are repelled by acid and migrate outwards, late procyclic forms remain at the inoculation site. pH taxis relies on cyclic AMP signalling. Acid sensing requires a flagellar adenylate cyclase, ACP5, and a cyclic AMP response protein, CARP3, that interacts with ACP5. Deletion of the flagellar phosphodiesterase PDEB1 abolishes pH taxis completely. Trypanosomes can also respond to exogenously formed gradients. pH sensing is likely to be biologically relevant as trypanosomes experience large differences in pH as they progress through their tsetse fly host. In addition, self-generated gradients may help reinforce directionality. Moreover, since trypanosomes encode a large family of adenylate cyclases, these may govern other chemotactic responses and tissue tropisms in both the mammal and the fly.

show abstract

Plasmodium vivax Strains Use Alternative Pathways for Invasion

Cited by 25 publications

References 18 publications

Home Sweet Home: Plasmodium vivax-Infected Reticulocytes—The Younger the Better?

Home Sweet Home: Plasmodium vivax-Infected Reticulocytes—The Younger the Better?

Leveraging genome editing to functionally evaluate Plasmodium diversity

Cyclic AMP signalling and glucose metabolism mediate pH taxis by African trypanosomes

Contact Info

Product

Resources

About