<i>Potyviridae</i>

L��pez-Moya, Juan Jos��; Valli, Adri��n; Garc��a, Juan Antonio

doi:10.1002/9780470015902.a0000755.pub2

Cited by 28 publications

(4 citation statements)

References 56 publications

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“…The family Potyviridae is a large group of plant viruses, many of which are of great agricultural significance (5,41). Viruses in this family have single-stranded positive-sense RNA (+ssRNA) genomes that are encapsidated in flexible, filamentous virions.…”

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confidence: 99%

Selection Pressure and Founder Effects Constrain Genetic Variation in Differentiated Populations of Soilborne Bymovirus Wheat yellow mosaic virus (Potyviridae) in China

Sun¹,

Sun²,

Tugume³

et al. 2013

Phytopathology®

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To study the population genetic structure and forces driving the evolution of Wheat yellow mosaic virus (WYMV), the nucleotide sequences encoding the coat protein (CP) (297 sequences) or the genome-linked virion protein (VPg) (87 sequences) were determined from wheat plants growing at 11 different locations distributed in five provinces in China. There were close phylogenetic relationships between all sequences but clustering on the phylogenetic trees was congruent with their provenance, suggesting an origin-dependent population genetic structure. There were low levels of genetic diversity, ranging from 0.00035 ± 0.00019 to 0.01536 ± 0.00043 (CP), and 0.00086 ± 0.00039 to 0.00573 ± 0.00111 (VPg), indicating genetic stability or recent emergence of WYMV in China. The results may suggest that founder effects play a role in shaping the genetic structure of WYMV. Between-population diversity was consistently higher than within-population diversity, suggesting limited gene flow between subpopulations (average FST 0.6241 for the CP and 0.7981 for the VPg). Consistent amino acid substitutions correlated with the provenance of the sequences were observed at nine positions in the CP (but none in the VPg), indicating an advanced stage in population structuring. Strong negative (purifying) selection was implicated on both the CP and VPg but positive selection on a few codons in the CP, indicating an ongoing molecular adaptation.

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mentioning

confidence: 99%

Selection Pressure and Founder Effects Constrain Genetic Variation in Differentiated Populations of Soilborne Bymovirus Wheat yellow mosaic virus (Potyviridae) in China

Sun¹,

Sun²,

Tugume³

et al. 2013

Phytopathology®

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“…The family Potyviridae [107] includes almost a quarter of the known plant viruses. The virions are approximately 7500 Å long and 120 Å in diameter, with helical pitches of about 33 Å.…”

Section: Potyvirusesmentioning

confidence: 99%

Fibre diffraction in the analysis of filamentous virus structure

Kendall

Stubbs

2015

Crystallography Reviews

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“…The genus Potyvirus, the largest group of plant viruses transmitted by aphids (Ló pez-Moya et al, 2009), is a good example. Potyvirus particles are thought to associate specifically with the lining of the anterior alimentary tract or mouthparts of aphids during transmission (Ammar et al, 1994; Wang et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

The helper-component protease transmission factor of tobacco etch potyvirus binds specifically to an aphid ribosomal protein homologous to the laminin receptor precursor

Fernández‐Calvino

Goytia

López‐Abella

et al. 2010

Journal of General Virology

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Potyviruses are plant pathogens transmitted by aphids in a non-persistent manner. During transmission, the virus-encoded factor helper-component protease (HCPro) is presumed to act as a molecular bridge, mediating the reversible retention of virions to uncharacterized binding sites in the vector mouthparts. Whilst the predicted interaction between HCPro and the coat protein (CP) of virions has been confirmed experimentally, the characterization of putative HCPro-specific receptors in aphids has remained elusive, with the exception of a report that described binding of HCPro of zucchini yellow mosaic virus to several cuticle proteins. To identify other aphid components that could play a role during transmission, this study used purified HCPro of tobacco etch virus (TEV) in far-Western blotting assays as bait to select interactors among proteins extracted from aphid heads. With this approach, new HCPro-interacting proteins were found, and several were identified after mass spectrometry analysis and searches in databases dedicated to aphid sequences. Among these interactors, a ribosomal protein S2 (RPS2) was chosen for further investigation due to its homology with the laminin receptor precursor, known to act as the receptor of several viruses. The specific interaction between RPS2 and TEV HCPro was confirmed after cloning and heterologous expression of the corresponding Myzus persicae gene. The possible involvement of RPS2 in the transmission process was further suggested by testing a variant of HCPro that was non-functional for transmission due to a mutation in the conserved KITC motif (EITC variant). This variant retained its ability to bind CP but failed to interact with RPS2. INTRODUCTIONNatural dissemination of most plant viruses is mediated by insect vectors, in either circulative or non-circulative processes, with aphids (Hemiptera, Aphididae) being the most common vectors (Pirone & Perry, 2002;Ng & Falk, 2006;Hogenhout et al., 2008). Whilst the viral components implicated in transmission are frequently well known, there is substantially less knowledge about the insect counterparts, and only a few published studies have identified insect products with the capacity to interact with virions or viral components that might play a role in plant virus transmission. Using far-Western blotting or similar methodologies, studies with circulative plant viruses have found proteins interacting with virions at key points of the pathways followed by these viruses inside their insect vectors. Examples include a thrips-transmitted tospovirus (Medeiros et al., 2000) and several aphid-transmitted members of the family Luteoviridae (van den Heuvel et al., 1997;Li et al., 2001;Seddas et al., 2004). A promising approach for further advances in this field is represented by the recent adoption of combined genetic and proteomic tools in the analysis of vector specificity in luteovirids (Yang et al., 2008).In the case of non-circulative plant viruses, the insect vector factors implicated in transmission remain largely unknown. The ...

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Potyviridae

Cited by 28 publications

References 56 publications

Selection Pressure and Founder Effects Constrain Genetic Variation in Differentiated Populations of Soilborne Bymovirus Wheat yellow mosaic virus (Potyviridae) in China

Selection Pressure and Founder Effects Constrain Genetic Variation in Differentiated Populations of Soilborne Bymovirus Wheat yellow mosaic virus (Potyviridae) in China

Fibre diffraction in the analysis of filamentous virus structure

The helper-component protease transmission factor of tobacco etch potyvirus binds specifically to an aphid ribosomal protein homologous to the laminin receptor precursor

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