<i>Rapid Communication</i>: Somatic Cell Nuclear Transfer in Humans: Pronuclear and Early Embryonic Development

Cibelli, José B.; Kiessling, Ann A.; Cunniff, Kerrianne; Richards, Charlotte J.; Lanza, Robert; West, Michael

doi:10.1089/152489001753262168

Cited by 83 publications

(62 citation statements)

References 26 publications

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“…Lu et al also reported having cloned human embryos using fetal fibroblasts and granulosa cells, although the reliability of the finding is uncertain since no DNA fingerprinting of the cloned blastocysts was provided [16]. Other preliminary reports have generated only early cleavage stage nuclear transfer embryos [17,18] using cytoplasts obtained from both in vitro and in vivo maturation and aged human in vitro fertilization (IVF) oocytes that had failed-to-fertilize [18,19]. Clearly, a number of significant hurdles need to be addressed if SCNT is to lead to the successful application of cell-based therapies using autologous hESC lines.…”

Section: Introductionmentioning

confidence: 99%

Development of Human Cloned Blastocysts Following Somatic Cell Nuclear Transfer with Adult Fibroblasts

French¹,

et al. 2008

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Nuclear transfer stem cells hold considerable promise in the field of regenerative medicine and cell-based drug discovery. In this study, a total of 29 oocytes were obtained from three young (20 -24 years old) reproductive egg donors who had been successful in previous cycles. These oocytes, deemed by intended parents to be in excess of their reproductive needs, were donated for research without financial compensation by both the egg donor and intended parents after receiving informed consent. All intended parents successfully achieved ongoing pregnancies with the oocytes retained for reproductive purposes. Mature oocytes, obtained within 2 hours following transvaginal aspiration, were enucleated using one of two methods, extrusion or aspiration, after 45 minutes of incubation in cytochalasin B. Rates of oocyte lysis or degeneration did not differ between the two methods. Somatic cell nuclear transfer (SCNT) embryos were constructed using two established adult male fibroblast lines of normal karyotype. High rates of pronuclear formation (66%), early cleavage (47%), and blastocyst (23%) development were observed following incubation in standard in vitro fertilization culture media. One cloned blastocyst was confirmed by DNA and mitochondrial DNA fingerprinting analyses, and DNA fingerprinting of two other cloned blastocysts indicated that they were also generated by SCNT. Blastocysts were also obtained from a limited number of parthenogenetically activated oocytes. This study demonstrates, for the first time, that SCNT can produce human blastocyst-stage embryos using nuclei obtained from differentiated adult cells and provides new information on methods that may be needed for a higher level of efficiency for human nuclear transfer. STEM CELLS 2008;26:485-493 Disclosure of potential conflicts of interest is found at the end of this article.

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Section: Introductionmentioning

confidence: 99%

Development of Human Cloned Blastocysts Following Somatic Cell Nuclear Transfer with Adult Fibroblasts

French¹,

et al. 2008

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“…Several previous studies have reported the successful activation of human oocytes and the formation of parthenogenetic blastocysts thereafter [10][11][12], but the successful derivation of parthenogenetic hESCs (pESCs) was reported only recently [13], owing to the inaccessibility of human oocytes and the existing ethical concerns about using viable fresh human oocytes in the process [14].…”

Section: Introductionmentioning

confidence: 99%

A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure

et al. 2007

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Homozygous human embryonic stem cells (hESCs) are thought to be better cell sources for hESC banking because their human leukocyte antigen (HLA) haplotype would strongly increase the degree of matching for certain populations with relatively smaller cohorts of cell lines. Homozygous hESCs can be generated from parthenogenetic embryos, but only heterozygous hESCs have been established using the current strategy to artificially activate the oocyte without second polar body extrusion. Here we report the first successful derivation of a human homozygous ESC line (chHES-32) from a one-pronuclear oocyte following routine in vitro fertilization treatment. chHES-32 cells express common markers and genes with normal hESCs. They have been propagated in an undifferentiated state for more than a year (>P50) and have maintained a stable karyotype of 46, XX. When differentiated in vivo and in vitro, chHES-32 cells can form derivatives from all three embryonic germ layers. The almost undetectable expression of five paternally expressed imprinted genes and their HLA genotype identical to the oocyte donor indicated their parthenogenetic origin. Using genome-wide single-nucleotide polymorphism analysis and DNA fingerprinting, the homozygosity of chHES-32 cells was further confirmed. The results indicated that 'unwanted' one-pronuclear oocytes might be a potential source for human homozygous and parthenogenetic ESCs, and suggested an alternative strategy for obtaining homozygous hESC lines from parthenogenetic haploid oocytes.

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“…Esto abrió un frente de discusión ética muy fuerte, mientras se gestaba una línea de resistencia contra la clonación, fuese reproductiva o terapéutica (17)(18)(19)(20)(21)(22)(23)(24). No obstante, hay consenso en que la clonación terapéutica favorecería el tratamiento de enfermedades invalidantes, como el mal de Alzheimer, el de Parkinson y la diabetes, entre otras (25)(26)(27).…”

Section: Clonación a Juicio Primero La Sentencia El Veredicto Desunclassified

Deterioro Genómico Y Manipulación Genética: Desequilibrio en La Prioridad De Las Agendas Públicas

González

Elío²

2007

Acta bioeth.

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Resumen: Se analiza el desequilibrio en el tratamiento de dos temas que atañen a la conservación de la integridad del genoma humano: las tecnologías de manipulación genética y el deterioro genómico provocado por la contaminación ambiental. Las carencias educativas, la falta de información asentada en los hechos y un manejo sensacionalista en los medios, entre otros factores, desajustan las agendas mediáticas con las necesidades públicas, lo que desemboca en una falta de conciencia ciudadana acerca de las verdaderas amenazas a las que está expuesto el material genético y los riesgos de salud que ello implica.Palabras clave: genotóxico, manipulación genética, deterioro genómico, bioética, toxicología ambiental, justicia ambiental GENOME DAMAGE AND GENETIC MANIPULATION. UNBALANCE IN PUBLIC POLICIES PRIORITYAbstract: Unbalance in the treatment of two topics related to the conservation of the human genome integrity is analyzed here: the technologies for genetic manipulation and genome damage due to environmental pollution. Lack of education and of information based on real facts, and the media sensationalist treatment, among other factors, provoke an unbalance between the media agenda and public needs. This leads to a lack of citizenship consciousness over the true threats that genetic material faces and the health risks that it involves.Keywords: genotoxic, genetic manipulation, genomic damage, bioethics, environmental toxicology, environment justice DETERIORIZAÇÃO GENÔMICA E MANIPULAÇÃO GENÉTICA. DESEQUILÍBRIO NA PRIORIDADE DAS AGENDAS PÚBLICASResumo: O presente texto analisa o desequilíbrio no tratamento de dois temas que dizem respeito à conservação da integridade do genoma humano: as tecnologias de manipulação genética e a deteriorização genômica provocada pela contaminação ambiental. As carências educativas, a falta de informação assentada nos fatos e um manejo sensacionalista dos meios, entre outros fatores, desajustam as agendas mediáticas com as necessidades públicas, o que desemboca numa falta de consciência cidadã em relação às verdadeiras ameaças à que se expôs o material genético e os riscos de saúde que isto implica.

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Rapid Communication: Somatic Cell Nuclear Transfer in Humans: Pronuclear and Early Embryonic Development

Cited by 83 publications

References 26 publications

Development of Human Cloned Blastocysts Following Somatic Cell Nuclear Transfer with Adult Fibroblasts

Development of Human Cloned Blastocysts Following Somatic Cell Nuclear Transfer with Adult Fibroblasts

A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure

Deterioro Genómico Y Manipulación Genética: Desequilibrio en La Prioridad De Las Agendas Públicas

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