<i>Rh6</i> gene modulates the visual mechanism of host utilization in fruit fly <scp><i>Bactrocera minax</i></scp>

Wang, Yaohui; Andongma, Awawing A.; Dong, Yongcheng; Chen, Zhenzhong; Xu, Penghui; Ren, Xueming; Krosch, Matt N.; Clarke, Anthony R.; Niu, Changying

doi:10.1002/ps.5278

Cited by 20 publications

(22 citation statements)

References 52 publications

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“…20E responsive genes including ecdysone nuclear receptor ecr and transcription factor broad were obtained by retrieving previously constructed B. minax transcriptomic data (Dong et al, 2014a). dsRNA preparation was performed following the methods previously described (Dong et al, 2016; Wang et al, 2018). The DNA templates for dsRNA synthesis were amplified by PCR using specific primers containing the T7 RNA polymerase promoter at both ends (Supplementary Table S1), following the program: 95°C for 5 min, followed by 30 cycles of 95°C for 30 s, 58°C for 30 s, and 72°C for 2 min, and a final extension step of 72°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Changes in Energy Metabolism Trigger Pupal Diapause Transition of Bactrocera minax After 20-Hydroxyecdysone Application

et al. 2019

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Correct timing of diapause entry and exit is critical for a species' survival. While many aspects of insect diapause are well-studied, the mechanisms underlying diapause termination remain largely unknown. The Chinese citrus fly, Bactrocera minax, is a univoltine insect with an obligatory pupal diapause. The application of 20-hydroxyecdysone (20E) is known to terminate diapause in B. minax, and we used this approach, along with isobaric tags for relative and absolute quantitation technology, to determine the proteins associated with diapause termination in this fly. Among 2,258 identified proteins, 1,169 proteins significantly differed at 1, 2, and 5 days post-injection of 20E, compared with the solvent-injected control group. Functional annotation revealed that the majority of differentially expressed proteins were enriched in the core energy metabolism of amino acids, proteins, lipids, and carbohydrates as well as in signal transduction pathways including PPAR signaling, Calcium signaling, Glucagon signaling, VEGF signaling, Ras signaling, cGMP-PKG signaling, and cAMP signaling. A combined transcriptomic and proteomic analysis suggested the involvement of energy metabolism in the response of diapause transition. RNA interference experiments disclosed that a 20E injection triggers diapause termination probably through non-genomic actions, rather than nuclear receptor mediated genomic actions. Our results provide extensive proteomic resources for insect diapause transition and offer a potential for pest control by incapacitating the regulation of diapause termination either by breaking diapause prematurely or by delaying diapause termination to render diapausing individuals at a high risk of mortality.

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Section: Methodsmentioning

confidence: 99%

Changes in Energy Metabolism Trigger Pupal Diapause Transition of Bactrocera minax After 20-Hydroxyecdysone Application

et al. 2019

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“…To fully understand the specific molecular mechanisms, the RNAi of BminOBP9 should be performed using the microinjector system to silence the functional genes in B. minax. 54,70,71 Silencing the GOBP99a gene of B. dorsalis influenced its egg-laying behavior. 34 Although B. minax is an oligophagous insect, it can lay eggs on almost all citrus species/cultivars, 72 which may release various VOCs depending on the developmental stages, tissues, and species or cultivars.…”

Section: Wileyonlinelibrarycom/journal/psmentioning

confidence: 99%

Characterization of the binding ability of the odorant binding protein BminOBP9 of Bactrocera minax to citrus volatiles

Yao

Zhao

Zhong

et al. 2020

Pest Management Science

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BACKGROUND Bactrocera minax, one of the most important citrus pests, oviposits exclusively on citrus fruit. In the insect olfactory system, odorant‐binding proteins (OBPs) facilitate the initial recognition role of host odor molecules. The aim of this study was to characterize the functional OBPs of B. minax and identify specific volatile organic compounds in the Citrus genus as OBP targets. RESULTS BminOBP9 (BminGOBP99a), a closely related homolog of BdorGOBP99a, which reduces the egg‐laying behavior of Bactrocera dorsalis through silencing technology, was cloned, expressed, and purified. The binding ability of BminOBP9 to 11 citrus volatiles was then examined using fluorescence competition binding assays (FCBA). The results demonstrated that BminOBP9 could bind to all tested citrus volatiles, as could BdorGOBP99a, ZcucGOBP99a, and ZtauGOBP99a. Interestingly, the binding ability of BminOBP9 was the strongest among the four, suggesting that BminOBP9 may have a function in the specific recognition of citrus volatiles. Furthermore, we aligned the above four proteins and found nine distinctive amino acid sites in BminOBP9. To identify the unique binding sites of BminOBP9, we produced the nine mutants using site‐directed mutagenesis. Further FCBA showed that the binding ability of the nine mutants to citrus volatiles significantly reduced, and six of them (substitutes S24P, L36F, E53K, N68D, D112A, and S118R) had the weakest binding ability. CONCLUSION The results demonstrated that BminOBP9 was the specific protein involved in the perception of citrus host volatiles by B. minax. Moreover, BminOBP9 could prove efficient in screening the candidate odors for pest management. © 2020 Society of Chemical Industry

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“…And B. minax experience a diapause when overwinter in the soil as pupae (Chen et al, 2016). To control this agricultural pest, a number of studies are currently being performed to understand the biology and ecology of B. minax (Xiong et al, 2016; Wang et al, 2018). However, there is still a lack of research on the bacterial and fungal compositions of the larval gut of B. minax , as well as on the shift patterns and functions of the core microbiota.…”

Section: Introductionmentioning

confidence: 99%

Similar Shift Patterns in Gut Bacterial and Fungal Communities Across the Life Stages of Bactrocera minax Larvae From Two Field Populations

Yao

Cai

et al. 2019

Front. Microbiol.

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Bactrocera minax (Enderlein) (Diptera: Tephritidae) is an oligophagous insect pest that damages citrus fruit, especially in China. Due to larvae living within a highly septic environment, a wide variety of microorganisms exist in the larval gut of B. minax. However, a systematic study of the intestinal microbiota of this harmful insect pest is still lacking. Here, we comprehensively investigated the larval gut microbiota of B. minax in two field populations from Zigui (developed in orange) and Danjiangkou (developed in mandarin orange). We observed a dominance of Proteobacteria and Firmicutes in these bacterial communities, and Enterobacteriaceae was the predominant family throughout the larval stage. However, most of the identified fungal sequences were annotated as being from either Ascomycota or Basidiomycota phyla. Although there was a difference in the structure of the microbial communities between the two populations, the dynamic change patterns of most of the members of the microbiota were similar across the lifespan of larvae in both populations. The relative abundances of the Acetobacteraceae, Leuconostocaceae, and Lactobacillaceae gut bacteria as well as the Pichiaceae, Sebacinaceae, and Amanitaceae fungi increased throughout development, and these microorganisms stably resided in the larval gut. Furthermore, the dynamic changes of the functions of gut bacterial communities were inferred, and there was a significant increase in carbohydrate metabolism across the lifespan of larvae in both groups. Spearman correlation analysis showed that Acetobacteraceae, Lactobacillaceae, and Leuconostocaceae displayed a positive correlation with fructose and mannose metabolism, an important pathway of carbohydrate metabolism, highlighting the potential roles of these prevalent microbial communities in host biology.

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Rh6 gene modulates the visual mechanism of host utilization in fruit fly Bactrocera minax

Cited by 20 publications

References 52 publications

Changes in Energy Metabolism Trigger Pupal Diapause Transition of Bactrocera minax After 20-Hydroxyecdysone Application

Changes in Energy Metabolism Trigger Pupal Diapause Transition of Bactrocera minax After 20-Hydroxyecdysone Application

Characterization of the binding ability of the odorant binding protein BminOBP9 of Bactrocera minax to citrus volatiles

Similar Shift Patterns in Gut Bacterial and Fungal Communities Across the Life Stages of Bactrocera minax Larvae From Two Field Populations

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