ROI-Finder: machine learning to guide region-of-interest scanning for X-ray fluorescence microscopy

Abstract

The microscopy research at the Bionanoprobe (currently at beamline 9-ID and later 2-ID after APS-U) of Argonne National Laboratory focuses on applying synchrotron X-ray fluorescence (XRF) techniques to obtain trace elemental mappings of cryogenic biological samples to gain insights about their role in critical biological activities. The elemental mappings and the morphological aspects of the biological samples, in this instance, the bacterium Escherichia coli (E. Coli), also serve as label-free biological fingerprints to identify E. coli cells that have been treated differently. The key limitations of achieving good identification performance are the extraction of cells from raw XRF measurements via binary conversion, definition of features, noise floor and proportion of cells treated differently in the measurement. Automating cell extraction from raw XRF measurements across different types of chemical treatment and the implementation of machine-learning models to distinguish cells from the background and their differing treatments are described. Principal components are calculated from domain knowledge specific features and clustered to distinguish healthy and poisoned cells from the background without manual annotation. The cells are ranked via fuzzy clustering to recommend regions of interest for automated experimentation. The effects of dwell time and the amount of data required on the usability of the software are also discussed.

2023 atomic spectrometry update – a review of advances in X-ray fluorescence spectrometry and its special applications

“…An open source machine-learning software package (ROI-Finder) was developed 33 for Bionanoprobe beamline 9-ID of the Advanced Photon Source (APS) (Lemont, Illinois, USA). In the analysis of samples containing biological cells treated with various chemicals, the algorithms distinguished the cells from the background and detected the different treatments.…”