Scutellaria barbata D. Don (SBD) protects oxygen glucose deprivation/reperfusion-induced injuries of PC12 cells by up-regulating Nrf2

Abstract

This study aimed to investigate the potential effect of Scutellaria barbata D. Don (SBD) on oxygen glucose deprivation/reperfusion (OGD/R)-injured PC12 cells. PC12 cells were pretreated with various concentrations of 0.1-0.8 mg/ml SBD for indicated times (12-48 h) and then subjected to OGD/R injury. Cell viability, apoptosis and proliferation were detected using MTT assay, flow cytometry, Ki67 staining and western blot. Oxidative damage was assessed by detecting MDA content, SOD activity and GSH levels. The mitochondrial membrane potential (Dwm) was measured by Rh123 staining. Western blot was performed to assess the expression levels of Nrf2 and PI3K/AKT pathway-related proteins. We found that SBD pretreatment promoted cell viability and proliferation but inhibited apoptosis of OGD/R-injured PC12 cells in dosage-and time-dependent manner. Meanwhile, SBD attenuated oxidative damage and restored mitochondria dysfunction, as evidenced by the reduced MDA content, the increased SOD and GSH levels, and the increased Dwm. Furthermore, SBD induced the expression of Nrf2 in a PI3K/AKT-dependent signalling. Knockdown of Nrf2 blocked the protective effects of SBD on PC12 cells. In conclusion, this study demonstrates that SBD pretreatment protects PC12 cells against OGD/R-induced injury. The potential mechanism may be through up-regulating the expression of Nrf2 in a PI3K/AKT-dependent pathway. ARTICLE HISTORY

Lion’s Mane Mushroom, Hericium erinaceus (Bull.: Fr.) Pers. Suppresses H2O2-Induced Oxidative Damage and LPS-Induced Inflammation in HT22 Hippocampal Neurons and BV2 Microglia

“…Antioxidant, mitochondrial health and anti-apoptosis are always interrelated in overcoming oxidative stress-induced toxicity, hence becoming the main targets of neuroprotective therapy [72,73,74,75,76]. Similarly, we found in this study that the increase in antioxidant activities were accompanied by improvement of MMP and reduction of mitochondrial toxicity.…”

Echinocystic acid alleviated hypoxic-ischemic brain damage in neonatal mice by activating the PI3K/Akt/Nrf2 signaling pathway

“…Moreover, our in vitro data also indicated that EA treatment suppressed OGD/R-induced ROS generation in primary cortical neurons. MDA, a final breakdown product of lipid peroxidation, is used to measure the extent of oxidative damage to cell membranes ( Wang et al, 2019 ). Our study showed that EA reduced the level of MDA in the ipsilateral hemisphere of neonatal mice following HIBD.…”

A Network-Based Method for Mechanistic Investigation and Neuroprotective Effect on Post-treatment of Senkyunolid-H Against Cerebral Ischemic Stroke in Mouse

“…In addition, Lv's data indicated that CXCL8 gene silencing accelerated the activation of neuroglial cells while suppressing neuroinflammation via the PI3K/Akt/NF-κB pathway in the mice of CIS (50). Besides, LY294002, as a PI3K inhibitor that is widely used for the study of the characteristics of PI3K cell signaling pathways (51)(52)(53), inhibited the expressions of PI3K/Akt/NF-κB pathway and blocked the function of SEH treatment on PC12 cells injured by OGD/R. For instance, published study indicated that ginkgolides protected against ischemia-reperfusion damage in vivo and in vitro through the activation of Nrf2 and CREB via PI3K/Akt signaling, which could be reversed by cotreatment with LY294002 (54).…”