<i>Spirulina</i> extract enriched for Braun‐type lipoprotein (Immulina®) for inhibition of 4T1 breast tumors' growth and metastasis

Kefayat, Amirhosein; Ghahremani, Fatemeh; Safavi, Ashkan; Hajiaghababa, Alireza; Moshtaghian, Jamal

doi:10.1002/ptr.6527

Cited by 11 publications

(9 citation statements)

References 76 publications

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“…Apoptosis rate was determined by the apoptosis detection kit (BD Biosciences, USA) according to previous study 52 . 4T1 cells were seeded in 6-well culture plates at a concentration of 1 × 10 5 and incubated overnight.…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo anti-cancer effects of hibernating common carp (Cyprinus carpio) plasma on metastatic triple-negative breast cancer

Golpich

Amini

Kefayat

et al. 2022

Sci Rep

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Uncontrollable proliferation is a hallmark of cancer cells. Cell proliferation and migration are significantly depressed during hibernation state. Many studies believe some factors in the plasma of hibernating animals cause these effects. This study aimed to assess the anti-cancer effects of hibernating common carp (Cyprinus carpio) plasma on 4T1 cancer cells in vitro and in vivo. The effect of hibernating plasma on cell viability, morphology, migration, apoptosis rate, and cell cycle distribution of 4T1 cells was investigated in vitro and in vivo. Hibernating plasma at a concentration of 16 mg/ml significantly reduced the viability of 4T1 cancer cells, without any toxicity on L929 normal fibroblast cells. It could change the morphology of cancer cells, induced apoptosis and cell cycle arrest at the G2/M phase, and inhibited migration. Furthermore, intratumoral injection of hibernating plasma (200 µl, 16 mg/ml) in the tumor-bearing mice caused a significant inhibition of 4T1 breast tumors volume (46.9%) and weight (58.8%) compared with controls. A significant decrease in the number of metastatic colonies at the lungs (80%) and liver (52.8%) of hibernating plasma-treated animals was detected which increased the survival time (21.9%) compared to the control groups. Immunohistochemical analysis revealed a considerable reduction in the Ki-67-positive cells in the tumor section of the hibernating plasma-treated animals compared with controls. Taken together, the SDS-PAGE and mass spectrometry analysis indicated the alpha-2-macroglobulin level in the hibernating fish plasma was significantly increased. It could exert an anti-cancer effect on breast cancer cells and suggested as a novel cancer treatment strategy.

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Section: Methodsmentioning

confidence: 99%

In vitro and in vivo anti-cancer effects of hibernating common carp (Cyprinus carpio) plasma on metastatic triple-negative breast cancer

Golpich

Amini

Kefayat

et al. 2022

Sci Rep

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“…High delivery efficiency, prolonged drug release, and low systemic toxicity are effective weapons for drug delivery systems to win the battle against metastatic breast cancer. It is demonstrated that Spirulina can be used as a natural carrier to build a drug-loaded system for targeted delivery and fluorescence imaging-guided chemotherapy on breast cancer lung metastases [ 92 ]. The Spirulina’s protein C-phycocyanin (C-PC) inhibited cell proliferation and reduced the colony formation ability of MDA-MB-231 cells.…”

Section: Applications In Oncologymentioning

confidence: 99%

“…Also, CD4, CD8, and CD56 staining were performed to investigate the effect on the immune cells’ recruitment to the tumors by immunohistochemistry. This treatment combination could significantly inhibit 4T1 breast tumors’ growth, decreasing the tumors’ volume compared to the control and the metastatic burden [ 92 ].…”

Section: Applications In Oncologymentioning

confidence: 99%

Wide Range Applications of Spirulina: From Earth to Space Missions

et al. 2022

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Spirulina is the most studied cyanobacterium species for both pharmacological applications and the food industry. The aim of the present review is to summarize the potential benefits of the use of Spirulina for improving healthcare both in space and on Earth. Regarding the first field of application, Spirulina could represent a new technology for the sustainment of long-duration manned missions to planets beyond the Lower Earth Orbit (e.g., Mars); furthermore, it could help astronauts stay healthy while exposed to a variety of stress factors that can have negative consequences even after years. As far as the second field of application, Spirulina could have an active role in various aspects of medicine, such as metabolism, oncology, ophthalmology, central and peripheral nervous systems, and nephrology. The recent findings of the capacity of Spirulina to improve stem cells mobility and to increase immune response have opened new intriguing scenarios in oncological and infectious diseases, respectively.

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“…Some of its derivatives are commercially in use as dietary supplements. For example, the company ChromaDex produces an extract called Immulina [ 135 , 136 , 137 ], with some action against IAV thanks to its Braun-type lipoproteins. These lipoproteins activate toll-like receptors, and consequently the immunity system [ 137 ].…”

Section: An Overview Of the Antiviral Effects Of A Supplementary Microalgae Diet And Its Possible Action On Sars-cov-2mentioning

confidence: 99%

“…Immurella, a mix of extracted polysaccharides from Chlorella, had interesting effects when supplemented into the diets of broilers [ 48 , 137 , 152 , 164 ].…”

Section: An Overview Of the Antiviral Effects Of A Supplementary Microalgae Diet And Its Possible Action On Sars-cov-2mentioning

confidence: 99%

Evaluation of Microalgae Antiviral Activity and Their Bioactive Compounds

et al. 2021

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During the last year, science has been focusing on the research of antivirally active compounds overall after the SARS-CoV-2 pandemic, which caused a great amount of deaths and the downfall of the economy in 2020. Photosynthetic organisms such as microalgae are known to be a reservoir of bioactive secondary metabolites; this feature, coupled with the possibility of achieving very high biomass levels without excessive energetic expenses, make microalgae worthy of attention in the search for new molecules with antiviral effects. In this work, the antiviral effects of microalgae against some common human or animal viruses were considered, focusing our attention on some possible effects against SARS-CoV-2. We summed up the data from the literature on microalgae antiviral compounds, from the most common ones, such as lectins, polysaccharides and photosynthetic pigments, to the less known ones, such as unidentified proteins. We have discussed the effects of a microalgae-based genetic engineering approach against some viral diseases. We have illustrated the potential antiviral benefits of a diet enriched in microalgae.

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Spirulina extract enriched for Braun‐type lipoprotein (Immulina®) for inhibition of 4T1 breast tumors' growth and metastasis

Cited by 11 publications

References 76 publications

In vitro and in vivo anti-cancer effects of hibernating common carp (Cyprinus carpio) plasma on metastatic triple-negative breast cancer

In vitro and in vivo anti-cancer effects of hibernating common carp (Cyprinus carpio) plasma on metastatic triple-negative breast cancer

Wide Range Applications of Spirulina: From Earth to Space Missions

Evaluation of Microalgae Antiviral Activity and Their Bioactive Compounds

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