Sweetpotato leaf curl virus decreased storage root yield and quality of edible sweetpotato in China

Abstract

Sweetpotato leaf curl virus (SPLCV) causes leaf curl symptoms in sweetpotato [Ipomoea batatas (L.) Lam]. These symptoms disappear under high temperature. Previous studies have shown that SPCLV reduces storage root yield in sweetpotato. However, knowledge of SPLCV's effect on root quality is limited. In this study, we surveyed the effect of SPLCV on storage root yield and quality in edible sweetpotato. Quantitative real-time reverse transcriptase-polymerase chain reaction and leaf paraffin section results showed that the SPLCV mRNA level and the distribution density of spongy mesophyll cells in SPLCV-infected plants were significantly higher than in healthy control plants (CK). Leaf net photosynthetic rate, vine length, and storage root yield were reduced in SPLCV-infected plants during the growing period, and the shoot/root ratio was significantly increased compared with CK. Additionally, root abscisic acid, auxin, and zeatin riboside contents under SPLCV infection were higher than the CK, whereas root gibberellic acid content was lower. Root dry matter, starch, and carotenoid contents were lower in the infected plants than in the CK. Moreover, SPLCV-infected plants showed reduced expression of starch and carotenoid metabolitic genes, such as ADP-glucose pyrophosphorylase-ß, granule-bound starch synthase I, starch branching enzyme I and II, and carotenoid isomerase, and increased expression of α-amylase, ß-carotene hydroxylase, and zeaxanthin epoxidase. These results suggest that sweetpotato infected by SPLCV had decreased storage root yield and decreased starch and carotenoid contents through reducing photosynthetic capacity, regulating starch and carotenoid degradation, and changing endogenous hormone contents. Therefore, effective management should be taken to prevent SPLCV epidemics during sweetpotato production.

Effect of infection of potato plants by Potato virus Y (PVY), Potato virus S (PVS), and Potato virus M (PVM) on content and physicochemical properties of tuber starch

“…As noted before, researchers found a decrease in the starch content in the tubers of potatoes infected with the virus (Blotskaya, 2000; Hou et al, 2020; Lal et al, 2021; Zhao et al, 2022; Amelyushkina, 2018; Rusetsky, 2006). The dry matter of tubers and starch content decrease in tubers of potato plants infected by PVM, PVS, and PVY.…”

“…Infection of potato plants with PLRV, PVY, PVS, and PVM leads to a decrease in the starch content in tubers by 0.5%–9% compared to uninfected tubers (Blotskaya, 2000). It is noted that various viruses, for example, sweet potato leaf curl virus (SPLCV) and Tomato leaf curl New Delhi virus‐potato (ToLCNDV‐potato) lead to a significant decrease in the starch content in the sweet potato (Hou et al, 2020) and potato tubers ( Solanum tuberosum L.) (Lal et al, 2021), respectively.…”

Activation of PsMYB10.2 Transcription Causes Anthocyanin Accumulation in Flesh of the Red-Fleshed Mutant of ‘Sanyueli’ (Prunus salicina Lindl.)

“…The total carotenoid content was measured using the carotenoid assay kit (Comin Biotechnology Co., Ltd., Suzhou, China.) as previously described ( Hou et al, 2020 ).…”

Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection

“…Leaf paraffin section analysis was performed as described by Hou et al [43]. The leaves were collected from the sixth leaf of N. benthamiana at 6 dpi and fixed in stationary liquid (v/v/v = 8/1/1, 50% ethanol/formal/glacial acetic acid) for 48 h. Samples were washed with water, dehydrated with 85, 95 and 100% ethanol and dimethylbenzene and embedded in paraffin.…”