“…For secondary antibodies, Alexa Fluor-568 anti-rabbit or anti-rat (Molecular Probes) was used at 1:1,000 dilutions for immunodetection. Pearson correlation coefficients were calculated from more than five individual cells by using a PSC colocalization plug-in of the ImageJ program (French et al, 2008;Wang et al, 2014;Gao et al, 2015), which produced r values in the range of 21 to +1, with 0 indicating no discernible correlation, and +1 and 21 indicating strong positive or negative correlations, respectively.…”