<i>Wolbachia</i>
            interferes with Zika virus replication by hijacking cholesterol metabolism in mosquito cells

Edwards, Brent; Ghedin, Elodie; Voronin, Denis

doi:10.1128/spectrum.02180-23

Cited by 6 publications

(3 citation statements)

References 50 publications

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“…These results suggest that w Au could show unique features in its inhibition of arboviruses compared to previously characterized Wolbachia strains. Further, Wolbachia was shown to interfere with Zika virus replication by hijacking cholesterol metabolism in mosquito cells [ 136 ].…”

Section: Pillar I—community Participationmentioning

confidence: 99%

Introduction of invasive mosquito species into Europe and prospects for arbovirus transmission and vector control in an era of globalization

Lühken,

Brattig,

Becker

2023

Infect Dis Poverty

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Background Mosquito research in Europe has a long history, primarily focused on malaria vectors. In recent years, invasive mosquito species like the Asian tiger mosquito (Aedes albopictus) and the spread of arboviruses like dengue virus, chikungunya virus or bluetongue virus have led to an intensification of research and monitoring in Europe. The risk of further dissemination of exotic species and mosquito-borne pathogens is expected to increase with ongoing globalization, human mobility, transport geography, and climate warming. Researchers have conducted various studies to understand the ecology, biology, and effective control strategies of mosquitoes and associated pathogens. Main body Three invasive mosquito species are established in Europe: Asian tiger mosquito (Aedes albopictus), Japanese bush mosquito (Ae. japonicus), and Korean bush mosquito (Aedes koreicus). Ae. albopictus is the most invasive species and has been established in Europe since 1990. Over the past two decades, there has been an increasing number of outbreaks of infections by mosquito-borne viruses in particular chikungunya virus, dengue virus or Zika virus in Europe primary driven by Ae. albopictus. At the same time, climate change with rising temperatures results in increasing threat of invasive mosquito-borne viruses, in particular Usutu virus and West Nile virus transmitted by native Culex mosquito species. Effective mosquito control programs require a high level of community participation, going along with comprehensive information campaigns, to ensure source reduction and successful control. Control strategies for container breeding mosquitoes like Ae. albopictus or Culex species involve community participation, door-to-door control activities in private areas. Further measures can involve integration of sterile insect techniques, applying indigenous copepods, Wolbachia sp. bacteria, or genetically modified mosquitoes, which is very unlike to be practiced as standard method in the near future. Conclusions Climate change and globalization resulting in the increased establishment of invasive mosquitoes in particular of the Asian tiger mosquito Ae. albopictus in Europe within the last 30 years and increasing outbreaks of infections by mosquito-borne viruses warrants intensification of research and monitoring. Further, effective future mosquito control programs require increase in intense community and private participation, applying physical, chemical, biological, and genetical control activities.

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Section: Pillar I—community Participationmentioning

confidence: 99%

Introduction of invasive mosquito species into Europe and prospects for arbovirus transmission and vector control in an era of globalization

Lühken,

Brattig,

Becker

2023

Infect Dis Poverty

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“…Perturbations in such pathways have been shown to be involved in Wolbachia -mediated antiviral activity against flaviviruses. The ability of the strains w Mel and w MelPop to block flaviviruses DENV and ZIKV in both Aedes aegypti and Aedes albopictus is at least partially due to changes in cholesterol dynamics and lipid metabolism [ 12 , 13 , 14 ]. It has been shown that strain w Au does not cause similar perturbations in Aedes cells/mosquitoes, and it is likely that they do not play an important role in the ability of w Au to block arbovirus replication [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Evidence of Differences in Cellular Regulation of Wolbachia-Mediated Viral Inhibition between Alphaviruses and Flaviviruses

Rainey,

Lefteri,

Darby

et al. 2024

Viruses

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The intracellular bacterium Wolbachia is increasingly being utilised in control programs to limit the spread of arboviruses by Aedes mosquitoes. Achieving a better understanding of how Wolbachia strains can reduce viral replication/spread could be important for the long-term success of such programs. Previous studies have indicated that for some strains of Wolbachia, perturbations in lipid metabolism and cholesterol storage are vital in Wolbachia-mediated antiviral activity against the flaviviruses dengue and Zika; however, it has not yet been examined whether arboviruses in the alphavirus group are affected in the same way. Here, using the reporters for the alphavirus Semliki Forest virus (SFV) in Aedes albopictus cells, we found that Wolbachia strains wMel, wAu and wAlbB blocked viral replication/translation early in infection and that storage of cholesterol in lipid droplets is not key to this inhibition. Another alphavirus, o’nyong nyong virus (ONNV), was tested in both Aedes albopictus cells and in vivo in stable, transinfected Aedes aegypti mosquito lines. The strains wMel, wAu and wAlbB show strong antiviral activity against ONNV both in vitro and in vivo. Again, 2-hydroxypropyl-β-cyclodextrin (2HPCD) was not able to rescue ONNV replication in cell lines, suggesting that the release of stored cholesterol caused by wMel is not able to rescue blockage of ONNV. Taken together, this study shows that alphaviruses appear to be inhibited early in replication/translation and that there may be differences in how alphaviruses are inhibited by Wolbachia in comparison to flaviviruses.

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“…Similarly, the gut bacterium Serratia marcescens facilitates DENV and ZIKV infection by secreting a bacterial effector that degrades glycosylated membrane-bound mucins on mosquito gut epithelia ( 13 ). Wolbachia , an endosymbiotic microorganism, also interrupts viral replication, possibly through the modulation of mosquito immune or physiological responses ( 14 , 15 ). Recent studies have demonstrated a substantial decrease in dengue transmission in areas where Wolbachia -infected mosquitoes were released as compared with that found in control areas ( 16 ).…”

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confidence: 99%

A naturally isolated symbiotic bacterium suppresses flavivirus transmission byAedesmosquitoes

Zhang,

Wang,

Shi

et al. 2024

Science

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The commensal microbiota of the mosquito gut plays a complex role in determining the vector competence for arboviruses. In this study, we identified a bacterium from the gut of field Aedes albopictus mosquitoes named Rosenbergiella sp. YN46 ( Rosenbergiella_ YN46) that rendered mosquitoes refractory to infection with dengue and Zika viruses. Inoculation of 1.6 × 10 3 colony forming units (CFUs) of Rosenbergiella_ YN46 into A. albopictus mosquitoes effectively prevents viral infection. Mechanistically, this bacterium secretes glucose dehydrogenase ( Ry GDH), which acidifies the gut lumen of fed mosquitoes, causing irreversible conformational changes in the flavivirus envelope protein that prevent viral entry into cells. In semifield conditions, Rosenbergiella_ YN46 exhibits effective transstadial transmission in field mosquitoes, which blocks transmission of dengue virus by newly emerged adult mosquitoes. The prevalence of Rosenbergiella_ YN46 is greater in mosquitoes from low-dengue areas (52.9 to ~91.7%) than in those from dengue-endemic regions (0 to ~6.7%). Rosenbergiella_ YN46 may offer an effective and safe lead for flavivirus biocontrol.

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Wolbachia interferes with Zika virus replication by hijacking cholesterol metabolism in mosquito cells

Cited by 6 publications

References 50 publications

Introduction of invasive mosquito species into Europe and prospects for arbovirus transmission and vector control in an era of globalization

Introduction of invasive mosquito species into Europe and prospects for arbovirus transmission and vector control in an era of globalization

Evidence of Differences in Cellular Regulation of Wolbachia-Mediated Viral Inhibition between Alphaviruses and Flaviviruses

A naturally isolated symbiotic bacterium suppresses flavivirus transmission byAedesmosquitoes

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