Ibuprofen removal in horizontal subsurface flow constructed wetlands: treatment performance and fungal community dynamics

Abstract

The treatment performance of ibuprofen (IBP)-enriched wastewater by horizontal subsurface flow constructed wetlands planted with cattail (Typha angustifolia) and unplanted control mesocosms was investigated. Removal efficiencies of IBP were significantly (p < .05) enhanced in the planted mesocosms (78.5%) compared to those in the unplanted beds (57.9%). An 18S rRNA gene high-throughput pyrosequencing approach was used to investigate the effects of IBP on the structure of the fungal community in these wetland systems. The overall diversity of the fungal community was reduced under the IBP exposure. Taxonomic analysis revealed that 62.2% of the fungal sequences were affiliated with Basidiomycota, followed by Ascomycota (37.4%) at the phylum level. Uncultured fungus (48.2%), Chaetomium sp. (14.2%), Aspergillus sp. (12.4%), Trichoderma sp. (5.7%), Cladosporium sp. (5.4%), and Emericellopsis sp. (5.2%) were identified as dominant genera. At the genus level, a distinct profile of the fungal community in the IBP-enriched mesocosms was observed as compared to the control beds, and as well specific fungal genera were enhanced in the planted beds, regardless of IBP enrichment. However, despite these differences, the composition of the fungal community (as measured by Bray-Curtis similarity) was mostly unaffected by the significant IBP enrichment. On the other hand, a consistent similarity pattern of fungal community structure in the planted mesocosms suggests that the presence of higher macrophytes in the wetland systems may well help shape the fungal community structure.

Wetlands for Wastewater Treatment

“…HSFCWs planted with cattail and unplanted control mesocosms showed a significant removal efficiency (p < .05), enhancing the planted mesocosms (78.5%) when compared to those in the unplanted beds (57.9%) (Zhang, Luo, et al, 2016). Three rural CWs used for the treatment of 32 multi-class PPCPs showed removal efficiencies that exhibited large variability between 11 and 100% for antiinflammatories, 37 and 99% for β-blockers, and 18 and 95% for diuretics ).…”

Characterization of microbial communities in wetland mesocosms receiving caffeine-enriched wastewater

“…Polymerase chain reaction (PCR) amplification was performed according to the methodology and conditions given in Zhang et al (2015).…”