2008
DOI: 10.1093/intimm/dxn051
|View full text |Cite
|
Sign up to set email alerts
|

ICAT expression disrupts  -catenin-TCF interactions and impairs survival of thymocytes and activated mature T cells

Abstract: T cell factor (TCF) family of transcription factors and beta-catenin critically regulate T cell development as demonstrated by the deletion of the tcf gene, which results in a block early in development that becomes complete in mice bearing tcf/lef double deletion. However, the role of beta-catenin, a major TCF cofactor, remains controversial. To directly address this, we have generated transgenic mice expressing Inhibitor of beta-catenin and TCF (ICAT), a naturally occurring inhibitor that specifically disrup… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
28
0
2

Year Published

2008
2008
2022
2022

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 29 publications
(30 citation statements)
references
References 38 publications
0
28
0
2
Order By: Relevance
“…The efficiency of transition was not significantly different from the WT (Fig 1F). To further confirm the results from CAT-KO mice, we employed another genetically modified mouse model that expresses an inhibitor of β-Catenin (ICAT) in thymocytes 34 . ICAT is a naturally occurring inhibitor of β-Catenin -TCF1 interactions and thus blocks the downstream activation of TCF1 specific genes 36 .…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…The efficiency of transition was not significantly different from the WT (Fig 1F). To further confirm the results from CAT-KO mice, we employed another genetically modified mouse model that expresses an inhibitor of β-Catenin (ICAT) in thymocytes 34 . ICAT is a naturally occurring inhibitor of β-Catenin -TCF1 interactions and thus blocks the downstream activation of TCF1 specific genes 36 .…”
Section: Resultsmentioning
confidence: 99%
“…β-Catenin knock out (CAT-KO) mice were generated by breeding previously described β-CAT flox/flox mice 33 with mice expressing Cre recombinase under the control of Cd4 promoter (CD4-Cre mice). ICAT (inhibitor of β-catenin-TCF interactions) transgenic (ICATtg) mice expressing ICAT under the control of proximal Lck promoter have been described previously 34 . PLZF-deficient mice (a kind gift of Dr. Derek Sant’Angelo) were described previously 35 .…”
Section: Methodsmentioning
confidence: 99%
“…Our results are in agreement with previous reports that TCF1 or b-catenin signaling is required for DP cell survival. 15,17,54 Increased survival may therefore promote tumor initiation by favoring the accumulation of secondary mutations.…”
Section: Discussionmentioning
confidence: 99%
“…10,13,14 Wnt signaling may also be important for cell survival, because CD4-Cre-mediated deletion of TCF1, or transgenic expression of catenin b interacting protein 1 (ICAT, an inhibitor of b-catenin/TCF interaction), reduces the survival of DP cells in the thymus, while overexpression of b-catenin enhances DP cell survival in vitro. [15][16][17] Surprisingly, loss of b-catenin in hematopoietic cells does not affect T-cell differentiation. 18,19 However, varying APC levels result in a gradient of developmental defects at the DN3 and DN4 stages.…”
Section: Cd44mentioning
confidence: 98%
“…Verificou-se também que camundongos deficientes em Wnt1 e 4 apresentam poucas células no timo (91). Ademais, a deficiência da proteína β-catenina inibe o desenvolvimento intratímico das células T (92) e a deleção de β-catenina ou a utilização de um de seus inibidores, o ICAT, limita o desenvolvimento dos timócitos nos estágios DN para DP (93) e diminui a sobrevivência dos timócitos DP (94). Adicionalmente, a ausência do fator de transcrição TCF-1 leva ao comprometimento no desenvolvimento dos timócitos (95), especialmente na transição do estágio DN1 para DN2 e DN4 para DP no timo (96).…”
Section: Polarização De Células Tcd4+unclassified