Ichthyenterobacterium magnum gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from olive flounder (Paralichthys olivaceus)

Abstract

Ichthyenterobacterium magnum gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from olive flounder (Paralichthys olivaceus) A novel marine bacterium isolated from the intestine of cultured flounder (Paralichthys olivaceus) was studied by using a polyphasic taxonomic approach. The isolate was Gram-stain-negative, pleomorphic, aerobic, yellow and oxidase-and catalase-negative. Phylogenetic analysis of 16S rRNA gene sequences indicated that isolate Th6T formed a distinct branch within the family Flavobacteriaceae and showed 96.6 % similarity to its closest relative, Bizionia hallyeonensis Ty7T . The DNA G+C content was 29 mol%. The major respiratory quinone was MK-6. The predominant fatty acids were iso-C 15 : 1 G, iso-C 15 : 0 , iso-C 15 : 0 3-OH, iso-C 17 : 0 3-OH and summed feature 3 (C 15 : 1 v6c and/or C 16 : 1 v7c). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, the novel bacterium has been assigned to a novel species of a new genus for which the name Ichthyenterobacterium magnum gen. nov., sp. nov. is proposed. The type strain is Th6 T (5JCM 18636 T 5KCTC 32140 T ).

Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes

“…The description is as before (Shakeela et al, 2015a) with the following modification. The G+C content of the type-strain genome is 31.4%, its approximate size 3.01 Mbp, its IMG deposit 2728369274.…”

“…Many Bacteroidetes produce pigments, particularly carotenoids (Goodwin, 1980; Sowmya and Sachindra, 2016) and flexirubin-like pigments (Reichenbach et al, 1974, 1980); in some cases proteorhodopsin has been observed (González et al, 2008; Yoshizawa et al, 2012). The cell shape ranges from short rods to long filaments (Saputra et al, 2015; Hahnke R. L. et al, 2016); longer cells are often flexible, including curved or helical shapes (Eder et al, 2015; Shakeela et al, 2015a). Spherical degenerative forms called “spheroblasts” are known from Flavobacterium (Bernardet and Bowman, 2006).…”

Pseudotenacibaculum haliotis gen. nov., sp. nov., a new member within the Tenacibaculum-Polaribacter clade of the family Flavobacteriaceae, isolated from the intestine of adult abalones, Haliotis diversicolor and H. discus hannai

“…Furthermore, the type species of the genus Tenacibaculum, T. maritimum (type strain NBRC 15946 T ), unexpectedly stands in a para-monophyletic clade from the other 23 species of this genus, more likely representing a novel genus which needs further examination in the future. In addition, Flavirhabdus iliipiscaria Th68 T , representing a recently described novel genus (Shakeela et al, 2015), formed a robust node with the genus Lacinutrix, and the 16S rRNA gene sequence showed 97.2 % similarity with the sequence of Lacinutrix venerupis Cmf 20.8 T . This indicated that strain Th68…”

Luteimonas arsenica sp. nov., an arsenic-tolerant bacterium isolated from arsenic-contaminated soil

“…Bacterial growth was evaluated at different temperatures (4, 10, 20, 28, 30, 35, 37 and 42 C). Growth was also evaluated at different pH values (pH 5.0-11.0) that were generated using the buffer systems Na 2 HPO 4 /citric acid (pH 5.0-7.0), Tris/ HCl (pH 8.0-9.0) and Na 2 CO 3 /NaHCO 3 (pH 9.0-11.0) (Shakeela et al, 2015). The results are shown in Table 1.…”