The objective of the present study was to explore the potential of avian hypophysis to induce the breeding of climbing perch. The pituitary glandof local chicken and duck and its combination was tested at the same dose of 0.5 ml/kg body weight (BW) of broodfish. Therefore, the tested treatments as follows; treatment A = 0.5 ml/kg BW of Ovaprim (control; Treatment B= 0.5 ml/kg BW of chicken pituitary extract; Treatment C= 0.5 ml/kg BW of duck pituitary extract; Treatment D= 0.25 ml/kg BW of chicken pituitary extract + 0.25 ml/kg BW of duck pituitary extract. The ANOVA test showed that treatment of avian pituitary extracts had a significant effect on the latency period, the total number of ovulatory eggs, fertility, hatchability, and survival of climbing perch larvae (P<0.05). The highest number of ovulated eggs was obtained in treatment B, this value was significantly different from treatments C and D. The highest fertility and survival were also obtained in the treatment B, but were not significantly different from the treatment C. Meanwhile, the highest hatchability was also obtained in treatment B and was significantly different from other treatments.