ICP-MS for specific detection in capillary electrophoresis

Álvarez-Llamas, Gloria; laCampa, Marı́a del Rosario Fernández de; Sanz‐Medel, Alfredo

doi:10.1016/j.trac.2004.09.007

Cited by 99 publications

(55 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The interface is considered to be a key factor for successfully coupling of CE and ICP-MS [20]. It is required to maintain a stable electrical circuit from CE to nebulizer, introduce CE effluent into ICP-MS efficiently and keep a low dead volume [21,22].…”

Section: Introductionmentioning

confidence: 99%

Speciation analysis of arsenic compounds by capillary electrophoresis on-line coupled with inductively coupled plasma mass spectrometry using a novel interface

Liu

Yun

et al. 2013

Journal of Chromatography A

View full text Add to dashboard Cite

Inductively coupled plasma mass spectrometry Simple and high efficient interface a b s t r a c tIn this work, a capillary electrophoresis (CE) coupled with inductively coupled plasma mass spectrometer (ICP-MS) system was developed for the simultaneous determination of ten arsenic compounds including arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenobetaine, arsenocholine, 3-nitro-4-hydroxyphenylarsonic acid, o-Arsanilic acid, p-ureidophenylarsonic acid, and 4-nitrophenylarsonic acid. The CE-ICP-MS system was hyphenated by a novel and high efficient interface which was directly used as the nebulizer. The separation was achieved on a 100 cm length × 50 m ID fused-silica capillary. Analytical conditions such as electrophoretic buffer composition, concentration and pH value, separation voltage, methanol concentration in the sheath flow liquid and sample injection time were optimized. Baseline separation of the ten arsenic species was achieved using an electrophoretic buffer consisting of 12 mM NaH 2 PO 4 and 8 mM HBO 3 at pH 9.20 with an applied voltage of +30 kV. The detection limits of the ten arsenic compounds ranged from 0.9 to 3.0 ng As g −1 , corresponding to absolute detection limits in the range of 19-65 fg As and the relative standard deviations (RSD, n = 5) were below 1.5%, 5.7% and 4.8% for migration time, peak height and peak area, respectively. This method was successfully applied to determine various arsenicals in two certified reference materials (TORT-2 and DORM-3) and environmental samples such as ground water samples, herbal plants and chicken meat.

show abstract

Section: Introductionmentioning

confidence: 99%

Speciation analysis of arsenic compounds by capillary electrophoresis on-line coupled with inductively coupled plasma mass spectrometry using a novel interface

Liu

Yun

et al. 2013

Journal of Chromatography A

View full text Add to dashboard Cite

show abstract

“…Recently the focus has shifted towards mass spectrometric detection such as ESI-MS [25,26] and inductively coupled plasma (ICP)-MS [27][28][29][30], opening new possibilities for the characterization of metallodrug interactions with biological targets [7,31]. Especially ICP-MS, the unrivalled detector in terms of specificity and LOD of metals bound to biomolecules, seems best-suited for quantification of adducts, especially if only present in traces [32,33].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis hyphenated to inductively coupled plasma‐mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

et al. 2008

View full text Add to dashboard Cite

Capillary electrophoresis hyphenated to inductively coupled plasma-mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasmaThe development of metal-based chemotherapeutics lacks methods which are capable of providing early indication on the potential of new metal complexes as future anticancer drugs. Since most of these compounds are administered intravenously, serum proteins are the first available biological binding partners in the bloodstream. For platinum-based anticancer drugs the interaction with serum proteins is regarded as an important contribution to the side effects accompanying chemotherapy. In contrast, newly developed ruthenium compounds are thought to be transported into the tumor in a protein-bound form. In here, the application of CE hyphenated to inductively coupled plasma (ICP)-MS, applying Polybrene-coated capillaries, is demonstrated for studying the interaction of indazolium [trans-tetrachlorobis(1H-indazole)ruthenate(III)] (KP1019) with HSA and transferrin, which are important transport proteins. Furthermore, the applicability of the method to human serum and plasma and, more importantly, to real-world patient samples was proven. KP1019 was found to bind to a high degree to HSA both in serum, plasma and the patient samples. Only minor fractions of ruthenium were found attached to other proteins.

show abstract

“…Chromatographic techniques coupled to element detectors are often the tools of choice for this kind of analysis. However, Capillary Electrophoresis (CE) is a powerful tool in element speciation because of its high separation capabilities and its environmentally friendly nature, which is due to the use of aqueous buffer solutions with moderate pH levels and its extremely low reagent/sample consumption [2][3][4]. The latter, however, presents an important problem when CE is coupled to Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) or Inductively Coupled Plasma Mass Spectrometry (ICP-MS) due to liquid flow incompatibility (typically one order of magnitude lower in CE than in ICP sample introduction systems nebulizers).…”

Section: Introductionmentioning

confidence: 99%

“…Hyphenations of ICP-MS with CE microfluidic devices have also been reported [15,16]. Most of the published reports have been recently reviewed by Álvarez-Llamas et al [4] and Michalke [17]. Commercial systems based on some of the abovementioned approached are nowadays offered by various leading manufacturers [18,19].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Elemental speciation by capillary electrophoresis with inductively coupled plasma spectrometry: A new approach by Flow Focusing® nebulization

Kovachev

Aguirre

Hidalgo

et al. 2014

Microchemical Journal

View full text Add to dashboard Cite

Please cite this article as: Nikolay Kovachev, MiguelÁngel Aguirre, Montserrat Hidalgo, Kiril Simitchiev, Violeta Stefanova, Veselin Kmetov, Antonio Canals, Elemental speciation by capillary electrophoresis with inductively coupled plasma spectrometry: A new approach by flow focusing nebulization, Microchemical Journal (2014Journal ( ), doi: 10.1016Journal ( /j.microc.2014 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. nebulizer as aerosol generation unit is presented, aiming to provide high signal sensitivity and low detection limits for element speciation at short analysis times. To prove its viability, the system prototype constructed has been coupled to an inductively coupled plasma -optical emission spectrometer (ICP-OES) and an inductively coupled plasma -mass spectrometer (ICP-MS) for Cr(III) and Cr(VI) speciation. Separationnebulization system and operation parameters (i.e., capillary length, nebulizer geometry, carrier flow, carrier ionic strength, separation potential and sample injection volume) have been considered and studied, and the analytical figures of merit obtained for model samples in ICP-MS are presented. The results obtained show that the developed instrumental system permits Cr speciation in less than two minutes with detection limits of 0.1, 0.2 and 0.03 µg/L for Cr(III), Cr(VI) and total Cr, respectively. A C C E P T E D M A N U S C R I P T

show abstract

ICP-MS for specific detection in capillary electrophoresis

Cited by 99 publications

References 52 publications

Speciation analysis of arsenic compounds by capillary electrophoresis on-line coupled with inductively coupled plasma mass spectrometry using a novel interface

Speciation analysis of arsenic compounds by capillary electrophoresis on-line coupled with inductively coupled plasma mass spectrometry using a novel interface

Capillary electrophoresis hyphenated to inductively coupled plasma‐mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

Elemental speciation by capillary electrophoresis with inductively coupled plasma spectrometry: A new approach by Flow Focusing® nebulization

Contact Info

Product

Resources

About