Identification and Analysis of a Degradation Product of the Glucocorticoid Desonide in Ointment.

Nguyen, Thuy T.; Kringstad, Randi; Aasen, Arne Jørgen; Rasmussen, Knut; Lönnberg, Harri; Berg, Jan-Eric; Bartók, Mihály; Pelczer, István; Dombi, György

doi:10.3891/acta.chem.scand.42b-0403

Cited by 5 publications

(4 citation statements)

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“…Comparing the quantification of D-LNC with ointment [62] is possible to realize that using the same mobile phase the peak of desonide came out in 7.9 min later obtained in the ointment which was around 4.0 min. However, ointment which indicates the presence of two peaks degradation products of desonide in times 1.0 and 2.0 min; probably the lipidcore nanocapsules provided protection from degradation.…”

Section: Discussionmentioning

confidence: 94%

Development and Physicochemical Characterization of Desonide-Loaded Nanocapsule Suspensions

Antonow

Lorenzoni

Barbosa

et al. 2016

Advances in Materials Science and Engineering

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Desonide is a topical corticosteroid that has been used for more than 30 years; however, its prolonged use can induce several side effects, affecting dermis and epidermis. The present work consists of development desonide-loaded nanocapsule suspensions (D-NC) using different polymers (Eudragit S100® or Eudragit L100®) and desonide-loaded lipid-core nanocapsules (D-LNC). They were formulated by interfacial deposition using the preformed polymer method and all formulations showed negative zeta potential and adequate nanotechnological characteristics (particle size 161–202 nm, polydispersity index < 0.20). Simple and sensitive methods using high-performance liquid chromatography (HPLC) were developed to quantify desonide in LNC and to study its release kinetics. The method was linear, specific, precise, and exact and therefore can be applied in quantification of D-NC and D-LNC. We evaluatedin vitromethods for drug release (dissolution, Franz diffusion cells, and dialysis sac) and we use mathematical models (monoexponential, biexponential, and Korsmeyer-Peppas) to show release kinetics from this system.

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Section: Discussionmentioning

confidence: 94%

Development and Physicochemical Characterization of Desonide-Loaded Nanocapsule Suspensions

Antonow

Lorenzoni

Barbosa

et al. 2016

Advances in Materials Science and Engineering

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“…The desonide HPLC ASSAY METHODOLOGY FOR A DESONIDE CREAM 1391 cream was prepared at a local pharmacy where it was purchased together with its individual components. The main degradation product was obtained as described by Nguyen et al (1988). The mixture under analysis contained 1 g of the desonide topical formulation in a mixture of chloroform and methanol (1:4).…”

Section: Experimental Chemicalsmentioning

confidence: 99%

“…Although it has been used for several decades, there are no official pharmacopeia methods to control the content of this active pharmaceutical ingredient in the topical formulations currently used (Sweetman 2007). Moreover, the analytical methods previously published regarding desonide containing formulations (DiPietra et al 1996;Nguyen et al 1988;Nguyen, Kringstad, and Rasmussen 1986;USP 1996) focus only on the non-simultaneous quantification of desonide (Nguyen et al 1988;USP 1996) and two preservatives, methylparaben and propylparaben (Nguyen et al 1986), or binary mixtures (DiPietra et al 1996). However, no methodology is available to quantify simultaneously desonide and different preservatives (such as antioxidant agents and other anti-microbial agents) included in several emulsions commercially available.…”

Section: Introductionmentioning

confidence: 97%

“…The method should also ensure the complete resolution between desonide and 11b-hydroxy-16a,17a-[(1-methylethylidene)bis(oxy)]-3-oxoandostra-1,4-diene-17b-carboxylic acid, obtained by oxidative cleavage of the a-cetol group, which is known to be the main degradation pathway of 21-hydroxyglucocorticoids (Nguyen et al 1988). The method should not resource to extraction, gradient elution, or derivatization procedures.…”

Section: Introductionmentioning

confidence: 99%

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Development of an HPLC Assay Methodology for a Desonide Cream with Chemometrics Assisted Optimization

et al. 2012

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A new reversed-phase liquid chromatographic method for the separation and simultaneous quantification of desonide, sorbic acid, methylparaben, propyl gallate, and the major degradation product of desonide in a hydrophilic cream was developed with the aid of experimental design, resolution loss functions, and chemometrics methods. A strategy involving a screening phase and a fractional factorial design revealed the most influent chromatographic variables (pH and organic solvent content). The arc tangent resolution function was adopted as the optimization loss function. These variables were further optimized using a central composite design. Multivariate curve resolution and partial least squares regression were tested to optimize the chromatographic run time. The latter revealed to be superior in terms of precision and allowed the validation of a method with a total run time 3 times lower (approximately 8 min). The experimental design and chemometrics models enabled an efficient use of time and resources in predicting the optimum separation conditions for the desonide formulation. The validation of the resulting method according to the current ICH guidelines confirmed its selectivity, linearity, accuracy, and precision.

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ChemInform Abstract: Identification and Analysis of a Degradation Product of the Glucocorticoid Desonide in Ointment.

et al. 1988

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Identification and Analysis of a Degradation Product of the Glucocorticoid Desonide in Ointment.

Cited by 5 publications

References 0 publications

Development and Physicochemical Characterization of Desonide-Loaded Nanocapsule Suspensions

Development and Physicochemical Characterization of Desonide-Loaded Nanocapsule Suspensions

Development of an HPLC Assay Methodology for a Desonide Cream with Chemometrics Assisted Optimization

ChemInform Abstract: Identification and Analysis of a Degradation Product of the Glucocorticoid Desonide in Ointment.

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