Identification and Analysis of Different Types of UFBs

Methylated histones on mitotic chromosomes promote topoisomerase IIα function for high fidelity chromosome segregation

“…TopoIIα prevents UFBs in anaphase by resolving catenated centromeric DNA. 19 , 20 Thus, we examined whether αChT and proper H3K27me3/H4K20me3 modifications contribute to this centromere TopoIIα function. We compared the TopoIIα replaced cell lines either before endogenous TopoIIα depletion (-Aux) or after depletion and induction of wt or ChTΔ (+Aux +Dox).…”