Identification and Analysis of Human Sex-Biased MicroRNAs

Cui, Chunmei; Yang, Weili; Shi, Jiangcheng; Zhou, Yong; Yang, Jichun; Cui, Qinghua; Zhou, Yuan

doi:10.1016/j.gpb.2018.03.004

Cited by 100 publications

(103 citation statements)

References 54 publications

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“…Speci cally, miRNA analysis across postnatal brain development revealed 40 miRNAs with signi cant sex-biased expression differences in the prefrontal cortex regions, 93% of them female-biased [40]. Further, investigation of four adult human tissues -brain, colorectal mucosa, peripheral blood, and cord blood -revealing 73 femalebiased and 163 male-biased expressed miRNAs [41]. Notably, two of 32 SON-associated miRNAs overlapped with miRNAs showing corresponding sex-biased expression in the adult brain, and two overlapped with miRNAs showing such a bias in the peripheral blood.…”

Section: Discussionmentioning

confidence: 99%

Variation of MicroRNA Expression in the Human Placenta Driven by Population Identity and Sex of the Newborn

Guo

Huang

Jiang

et al. 2020

Preprint

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BackgroundAnalysis of lymphocyte cell lines revealed substantial differences in the expression of mRNA and microRNA (miRNA) among human populations. The extent of such population-associated differences in actual human tissues remains largely unexplored. The placenta is one of the few solid human tissues that can be collected in substantial numbers in a controlled manner, enabling quantitative analysis of transient biomolecules such as RNA transcripts. Here, we analyzed microRNA (miRNA) expression in human placental samples derived from 36 individuals representing four genetically distinct human populations: African Americans, European-Americans, South Asians, and East Asians. All samples were collected at the same hospital following a unified protocol, thus minimizing potential biases that might influence the results.ResultsSequence analysis of the miRNA fraction yielded 938 annotated and 70 novel miRNA transcripts expressed in the placenta. Of them, 82 (9%) of annotated and 11 (16%) of novel miRNAs displayed quantitative expression differences among populations, generally reflecting reported genetic and mRNA-expression-based distances. Several co-expressed miRNA clusters stood out from the rest of the population-associated differences in terms of miRNA evolutionary age, tissue-specificity, and disease-association characteristics. Among three non-environmental influenced demographic parameters, the second largest contributor to miRNA expression variation after population was the sex of the newborn, with 32 miRNAs (3% of detected) exhibiting significant expression differences depending on whether the newborn was male or female. Male-associated miRNAs were evolutionarily younger and correlated inversely with the expression of target mRNA involved in neuron-related functions. In contrast, both male and female-associated miRNAs appeared to mediate different types of hormonal responses. Demographic factors further affected reported imprinted expression of 66 placental miRNAs: the imprinting strength correlated with the mother’s weight, but not height. ConclusionsOur results showed that among 12 assessed demographic variables, population affiliation and fetal sex had a substantial influence on miRNA expression variation among human placental samples. The effect of newborn-sex-associated miRNA differences further led to expression inhibition of the target genes clustering in specific functional pathways. By contrast, population-driven miRNA differences might mainly represent neutral changes with minimal functional impacts.

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Section: Discussionmentioning

confidence: 99%

Variation of MicroRNA Expression in the Human Placenta Driven by Population Identity and Sex of the Newborn

Guo

Huang

Jiang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition, arterial stiffness measures were obtained 10 min after exercise, so more immediate effects of exercise may have been missed ( Mutter et al., 2017). There are reported effects of sex, CVD risk factors, and training status on circulating microRNAs and arterial stiffness, so only young, healthy men were included as participants in this initial study, limiting the generalizability of our results to other populations (Ameling et al., 2015; Bye et al., 2013; Cui et al., 2018; Jones Buie, Goodwin, Cook, Halushka, & Fan, 2016).…”

Section: Discussionmentioning

confidence: 99%

Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise

et al. 2020

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High-intensity interval (HII) exercise elicits distinct vascular responses compared to a matched dose of moderate intensity continuous (MOD) exercise. However, the acute effects of HII compared to MOD exercise on arterial stiffness are incompletely understood. Circulating microRNAs (ci-miRs) may contribute to the vascular effects of exercise. We sought to determine exercise intensity-dependent changes in ci-miR potentially underlying changes in arterial stiffness. Ten young, healthy men underwent well-matched, 30-min HII and MOD exercise bouts. RT-qPCR was used to determine the levels of seven vascular-related ci-miRs in serum obtained immediately before and after exercise. Arterial stiffness measures including carotid to femoral pulse wave velocity (cf-PWV), carotid arterial compliance and β-stiffness, and augmentation index (AIx and AIx75) were taken before, 10min after and 60min after exercise. Ci-miR-21-5p, 126-3p, 126-5p, 150-5p, 155-5p, and 181b-5p increased after HII exercise (p < .05), while ci-miR-150-5p and 221-3p increased after MOD exercise (p = .03 and 0.056). One hour after HII exercise, cf-PWV trended toward being lower compared to baseline (p = .056) and was significantly lower compared to 60min after MOD exercise (p = .04). Carotid arterial compliance was increased 60min after HII exercise (p = .049) and was greater than 60min after MOD exercise (p = .02). AIx75 increased 10 min after both HII and MOD exercise (p < .05).There were significant correlations between some of the exercise-induced changes in individual ci-miRs and changes in cf-PWV and AIx/AIx75. These results support the hypotheses that arterial stiffness and ci-miRs are altered in an exercise intensitydependent manner, and ci-miRs may contribute to changes in arterial stiffness.

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“…MicroRNA (miRNA) are short, single stranded non-coding RNA that bind complementary sequences on target genes and block mRNA translation and degradation. Roughly 14% of all miRNA show a sex-biased expression pattern ( 84 ). There are 113 miRNA on the X chromosome and 2 miRNA on the Y chromosome ( 4 ).…”

Section: Immune Responsementioning

confidence: 99%

What’s Sex Got to Do With COVID-19? Gender-Based Differences in the Host Immune Response to Coronaviruses

et al. 2020

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The novel severe acute respiratory syndrome coronavirus 2, the cause of the coronavirus disease 2019 (COVID-19) pandemic, has ravaged the world, with over 22 million total cases and over 770,000 deaths worldwide as of August 18, 2020. While the elderly are most severely affected, implicating an age bias, a striking factor in the demographics of this deadly disease is the gender bias, with higher numbers of cases, greater disease severity, and higher death rates among men than women across the lifespan. While pre-existing comorbidities and social, behavioral, and lifestyle factors contribute to this bias, biological factors underlying the host immune response may be crucial contributors. Women mount stronger immune responses to infections and vaccinations and outlive men. Sex-based biological factors underlying the immune response are therefore important determinants of susceptibility to infections, disease outcomes, and mortality. Despite this, gender is a profoundly understudied and often overlooked variable in research related to the immune response and infectious diseases, and it is largely ignored in drug and vaccine clinical trials. Understanding these factors will not only help better understand the pathogenesis of COVID-19, but it will also guide the design of effective therapies and vaccine strategies for gender-based personalized medicine. This review focuses on sex-based differences in genes, sex hormones, and the microbiome underlying the host immune response and their relevance to infections with a focus on coronaviruses.

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Identification and Analysis of Human Sex-Biased MicroRNAs

Cited by 100 publications

References 54 publications

Variation of MicroRNA Expression in the Human Placenta Driven by Population Identity and Sex of the Newborn

Variation of MicroRNA Expression in the Human Placenta Driven by Population Identity and Sex of the Newborn

Changes in circulating microRNA and arterial stiffness following high‐intensity interval and moderate intensity continuous exercise

What’s Sex Got to Do With COVID-19? Gender-Based Differences in the Host Immune Response to Coronaviruses

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