Identification and antimicrobial susceptibility testing of anaerobic bacteria isolated from clinical samples

Abstract

Background/aims: Anaerobic bacteria have attracted the attention of scientists as the causative agents of serious infections since the 19th century. Antibiotic resistance has become a major problem among these bacteria, but antibiotic susceptibility testing has not been standardized yet. Materials and methods:In this study, anaerobic bacteria isolated from a total of 800 clinical materials sent to our laboratory were evaluated. A total of 69 anaerobic bacteria isolated were identified with VITEK 2 (Biomerieux). Antibiotic susceptibility testing was performed on 23 of 69 isolates with gradient test method. Results: Identified anaerobic bacteria were Prevotella spp. (19 isolates, 27.5%), Veillonella spp. (14 isolates, 20.2%), Bacteroides spp. (9 isolates, 13%), Peptoniphilus spp. (6 isolates, 8.6%), Parvimonas spp. (5 isolates, 7.2%), Fusobacterium spp. (3 isolates, 4.3%), Actinomyces spp. (3 isolates, 4.3%), Parabacteroides spp. (2 isolates, 2.8%), Finegoldia magna (2 isolates, 2.8%), Clostridium spp. (2 isolates, 2.8%), Propionibacterium spp. (1 isolate, 1.4%), Peptostreptococcus spp. (1 isolate, 1.4%), Bifidobacterium spp. (1 isolate, 1.4%) and Lactobacillus spp. (1 isolate, 1.4%). Resistance rates of anaerobic bacteria to metronidazole, imipenem, penicillin, clindamycin and cefoxitin were found as %95.