Identification and association of single-nucleotide polymorphisms in gonadotropin-inhibitory hormone (GnIH) gene with egg production traits in Erlang mountainous chickens

Abstract

ABSTRACT. Gonadotropin-inhibitory hormone (GnIH) gene is an important gene in reproduction. In this study, we screened singlenucleotide polymorphisms (SNPs) in the chicken GnIH gene among 204 individuals in Erlang mountainous chickens. We then analyzed the associations between polymorphisms of the GnIH gene and 5 egg production traits in chickens. Five SNPs (T3305C, T3310C, G3403C, G3411A, and T3591C) were detected. Associations between polymorphic loci and age at first egg, body weight at first egg, weight at first egg, egg weight in 300 days, and egg production in 300 days were analyzed using analysis of covariance. The results showed that SNP1, SNP3, and SNP4 had large effects on age at first egg, while SNP5 had a large effect on body weight at first egg; of the effect of the TT genotype was significantly higher than that of CT (P < 0.01). Further 295 Association of GnIH with egg production traits ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 294-303 (2015) analysis show that the highest frequency (0.2353) haplotype H1H1 was associated with the latest age at first egg. The H4H5 haplotype had a positive effect on egg production in 300 days and a negative effect on weight at first egg. We observed no association between the H3H3 haplotype and body weight at first egg.

Molecular, cellular, morphological, physiological and behavioral aspects of gonadotropin-inhibitory hormone

“…Five SNPs were detected. There were significant associations between polymorphic loci and age at first egg, body weight at first egg and egg production in 300 days (Hu et al, 2015). Lima et al (2014) reported that there is a three-nucleotide in frame deletion in the human GnIH precursor gene (p.I71_K72), with a smaller proportion in the idiopathic central precocious puberty (CPP) (5%) compared to the normosmic isolated hypogonadotrophic hypogonadism (nIHH) (15%) group.…”

“…Genetic variations are commonly found in the DNA between the genes, but SNPs within a gene or in a regulatory region may directly affect the gene's function. Hu et al (2015) screened SNPs in the chicken GnIH precursor gene among 204 individuals in Erlang mountainous chickens. They further analyzed the associations between polymorphisms in the GnIH precursor gene and egg production traits.…”

Association Analysis Between Polymorphism of Gonadotrophin Releasing Hormone Genes and Growth Traits of Quail (Coturnix Coturnix)

“…Peng L. (2013) detected AA, AB and BB genotypes on GR1 segment of intron 2 of GnRHR gene in muscovy duck, which all showed significant correlations with laying start age, maximum number of continuous laying days, average number of continuous laying days and egg number of 300-day old ducks. Hu et al (2015) pointed out that 3 SNPs of GnRH gene influenced laying start age of Erlangshan chicken significantly and SNP5 played an important role in body weight at the beginning of laying. All these studies show that GnRH gene might regulate the production performance of poultry.…”

Association Analysis between Polymorphism of GnRH-1 Genes and Carcass Traits of Quail (Coturnix coturnix)

“…For mutation site T68G of GnRH gene detected in Rongjiang little sweet sheep, lambing number of GG genotype was far higher than those of the rest two genotypes (P<0.05) (Li et al 2016). That 3 SNPs of GnRH gene influenced laying start age of Erlangshan chicken significantly and SNP5 played an important role in body weight at beginning of laying (Hu et al 2015). All These studies showed that GnRH gene could regulate the production performance of poultry.…”