Identification and biofilm development by a new fungal keratitis aetiologic agent

Calvillo‐Medina, Rosa Paulina; Martínez-Neria, Magda; Mena‐Portales, Julio; Barba‐Escoto, Luis; Raymundo, Tania; Campos-Guillén, Juan; Jones, George H.; Reyes‐Grajeda, Juan Pablo; González-y-Merchand, Jorge A.; Lucio, Víctor Manuel Bautista-de

doi:10.1111/myc.12849

Cited by 23 publications

(23 citation statements)

References 55 publications

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“…Recently, using sequencing of the ITS1+5.8S+ITS2 rDNA region, or of a fragment of the 28S large subunit ribosomal (LSU), it has been possible to recognize the species N. novaehollandiae , N. orchidaceous , N. dimidiatum , and N. oculus [3,11]. In the present study, the high level of accuracy of using the ITS1+5.8S+ITS2 rDNA region to identify N. dimidiatum reveals that this barcode is fit-for-propose in the clinical field.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, within this genus, four species are recognized: N. novaehollandiae , N. orchidacearum , N. oculus , and N. dimidiatum [2,3]. The first two species have been reported as phytopathogens, while N. oculus and N. dimidiatum have been associated to ocular or keratinized tissue (skin or nails) infections, which are indistinguishable from dermatophytosis [3,4,5]. Recently, it has been demonstrated that N. oculus can form biofilms and cause hemolysis [3].…”

Section: Introductionmentioning

confidence: 99%

“…The first two species have been reported as phytopathogens, while N. oculus and N. dimidiatum have been associated to ocular or keratinized tissue (skin or nails) infections, which are indistinguishable from dermatophytosis [3,4,5]. Recently, it has been demonstrated that N. oculus can form biofilms and cause hemolysis [3]. In contrast, N. dimidiatum can also cause subcutaneous and deep infections, mainly in immunocompromised patients [6,7,8].…”

Section: Introductionmentioning

confidence: 99%

“…The identification of this genus has been based on the observation of macroscopic and microscopic characteristics of in vitro cultures [3]. Initially, white colonies are observed in the culture media, but with age, they become black/greenish, and the plate is fully covered with powdery aerial mycelium.…”

Section: Introductionmentioning

confidence: 99%

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ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)

et al. 2019

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Within the Neoscytalidium genus, N. dimidiatum, N. oculus, N. orchidacearum, and N. novaehollandiae have been recognized. Although these species are frequently found in soil, N. dimidiatum has been identified as an etiologic agent of onychomycosis or dermatomycosis, and N. oculus has been identified as an etiologic agent of an ocular lesion. All these species can be cultured in vitro, but their morphological identification by macroscopic and microscopic traits is difficult and imprecise due to their similarity. In this study, 34 isolates of Neoscytalidium spp. from 32 onychomycosis and two dermatomycosis cases in Medellin (Colombia) were identified at the species level using sequencing of the ITS1+5.8S+ITS2 nuclear rDNA region and MALDI-TOF mass spectrometry (MS). Neoscytalidium dimidiatum strain MUM 17.21 was used to construct the reference spectrum in the in-house library to identify the clinical isolates by MALDI-TOF MS. Additionally, N. dimidiatum PPC-216 and PLAB-055 strains were used to validate the in-house constructed reference spectra. Although four groups were observed in the dendrogram obtained from the proteins of each isolate profile, MALDI-TOF MS and sequencing results are in accordance, since all isolates were identified as N. dimidiatum.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)

et al. 2019

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“…In Mexico, fungal keratitis is a poorly documented and understood problem, which is mainly studied in hospitals 1 . There are few reports regarding keratomycosis as health problem.…”

Section: Introductionmentioning

confidence: 99%

Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals

Calvillo‐Medina

Ponce-Angulo

Raymundo

et al. 2020

Mycoses

Self Cite

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BackgroundInfectious keratitis is the main cause of preventable blindness worldwide, with about 1.5–2.0 million new cases occurring per year. This inflammatory response may be due to infections caused by bacteria, fungi, viruses or parasites. Fungal keratitis is a poorly studied health problem.ObjectivesThis study aimed to identify a new fungal species by molecular methods and to explore the possible efficacy of the three most common antifungals used in human keratitis in Mexico by performing in vitro analysis. The capacity of this pathogen to cause corneal infection in a murine model was also evaluated.MethodsThe fungal strain was isolated from a patient with a corneal ulcer. To identify the fungus, taxonomic and phylogenetic analyses (nrDNA ITS and LSU data set) were performed. An antifungal susceptibility assay for amphotericin B, itraconazole and voriconazole was carried out. The fungal isolate was used to develop a keratitis model in BALB/c mice; entire eyes and ocular tissues were preserved and processed for histopathologic examination.Results and conclusionThis fungal genus has hitherto not been reported with human keratitis in Mexico. We described a new species Purpurecillium roseum isolated from corneal infection. P roseum showed resistance to amphotericin B and itraconazole and was sensitive to voriconazole. In vivo study demonstrated that P roseum had capacity to developed corneal infection and to penetrate deeper corneal tissue. The global change in fungal infections has emphasised the need to develop better diagnostic mycology laboratories and to recognise the group of potential fungal pathogens.

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Curvularia, Exophiala, Scedosporium, Sporothrix, and Other Melanized Fungi

Guarro,

Madrid,

de Hoog

2023

ClinMicroNow

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This chapter covers most of the agents of phaeohyphomycosis, chromoblastomycosis, and sporotrichosis, as well as a number of agents of superficial and cutaneous disease. Pleoanamorphism is particularly striking in members of the black yeasts and in the genera Scedosporium and Triadelphia . Members of the genus Curvularia usually produce elongated conidia that are inconspicuously distoseptate and often possess an intermediate, asymmetrically swollen cell that gives the conidia a typically curved appearance. Exophiala is the main genus of clinically relevant black yeasts. Sporothrix brasiliensis is highly virulent and has caused large epidemics in Brazil; transmission occurs particularly through stray cats. Dematiaceous fungi are able to cause superficial infections, such as those of the skin and nails. Some melanized phialidic fungi were segregated from Phialophora on molecular and morphological grounds; these fungi now constitute small islands of clinical significance in the order Calosphaeriales, which otherwise contains plant‐associated moulds.

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Identification and biofilm development by a new fungal keratitis aetiologic agent

Cited by 23 publications

References 55 publications

ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)

ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)

Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals

Curvularia, Exophiala, Scedosporium, Sporothrix, and Other Melanized Fungi

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