Identification and Characterization of a Novel Thymus Aging Related Protein Rwdd1

Kang, Ning; Duan, Lian; Tang, Long; Liu, Shirong; Li, Chunman; Li, Yonghai; Li, Qingfeng; Hu, Yu; Cui, Lianxian; Wang, He

doi:10.1038/cmi.2008.34

Cited by 9 publications

(8 citation statements)

References 26 publications

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“…The same reduction is shown dealing with the proliferative cell staining with Ki67; 3-fold more is the level of apoptotic TECs in aged mice thymus (Gui et al 2007). Kang et al (2008) discovered an androgen receptor (AR) RWD domain containing 1 (Rwdd1) that is markedly decreased in aged and oxidatively stressed mice on thymocytes and TECs. AR is connected with the thymus involution.…”

Section: Murine Thymic Involutionmentioning

confidence: 59%

Thymus and aging: morphological, radiological, and functional overview

Rezzani

Nardo

Favero

et al. 2013

AGE

158

112

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Aging is a continuous process that induces many alterations in the cytoarchitecture of different organs and systems both in humans and animals. Moreover, it is associated with increased susceptibility to infectious, autoimmune, and neoplastic processes. The thymus is a primary lymphoid organ responsible for the production of immunocompetent T cells and, with aging, it atrophies and declines in functions. Universality of thymic involution in all species possessing thymus, including human, indicates it as a long-standing evolutionary event. Although it is accepted that many factors contribute to age-associated thymic involution, little is known about the mechanisms involved in the process. The exact time point of the initiation is not well defined. To address the issue, we report the exact age of thymus throughout the review so that readers can have a nicely pictured synoptic view of the process. Focusing our attention on the different stages of the development of the thymus gland (natal, postnatal, adult, and old), we describe chronologically the morphological changes of the gland. We report that the thymic morphology and cell types are evolutionarily preserved in several vertebrate species. This finding is important in understanding the similar problems caused by senescence and other diseases. Another point that we considered very important is to indicate the assessment of the thymus through radiological images to highlight its variability in shape, size, and anatomical conformation.

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Section: Murine Thymic Involutionmentioning

confidence: 59%

Thymus and aging: morphological, radiological, and functional overview

Rezzani

Nardo

Favero

et al. 2013

AGE

158

112

View full text Add to dashboard Cite

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“…TGFβRII [TGF (transforming growth factor)-β type II receptor] gene expression was also not significantly different between the three groups ( Figure 5E). Rwdd1 (Rwd-domain-containing 1) is a recently characterized thymus aging-related protein that has been suggested to be indirectly involved in AR signalling [26]. The expression level of Rwdd1 in the thymus from PLP and recuperated animals was similar to controls ( Figure 5F).…”

Section: Genes Involved In Thymic Growth and Agingmentioning

confidence: 75%

Early-life nutrition influences thymic growth in male mice that may be related to the regulation of longevity

et al. 2009

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Nutrition and growth rate during early life can influence later health and lifespan. We have demonstrated previously that low birthweight, resulting from maternal protein restriction during pregnancy followed by catch-up growth in rodents, was associated with shortened lifespan, whereas protein restriction and slow growth during lactation increased lifespan. The underlying mechanisms by which these differences arise are unknown. In the present study, we report that maternal protein restriction in mice influences thymic growth in early adult life. Offspring of dams fed a low-protein diet during lactation (PLP offspring) had significant thymic growth from 21 days to 12 weeks of age, whereas this was not observed in control mice or offspring of dams fed a low-protein diet during pregnancy (recuperated offspring). PCNA (proliferating-cell nuclear antigen) and SIRT1 (silent information regulator 1) protein levels at 21 days of age were significantly higher in the thymus from both PLP mice (P<0.001 and P<0.05 respectively) and recuperated mice (P<0.001 and P<0.01 respectively) compared with controls. At 12 weeks, PLP mice maintained a higher SIRT1 level, whereas PCNA was decreased in the thymus from recuperated offspring. This suggests that mitotic activity was initially enhanced in the thymus from both PLP and recuperated offspring, but remained sustained into adulthood only in PLP mice. The differential mitotic activity in the thymus from PLP and recuperated mice appeared to be influenced by changes in sex hormone concentrations and the expression of p53, p16, the androgen receptor, IL-7 (interleukin-7) and the IL-7 receptor. In conclusion, differential thymic growth may contribute to the regulation of longevity by maternal diet.

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“…However, due to the complexity of the aging process, the underlying mechanisms of age-related thymus involution are not well understood. To investigate the gene expression patterns in thymuses from aged mice, we performed differential display reverse (1) transcriptase polymerase chain reaction (DDRT-PCR) using total RNA from thymus of 1-month and 10-month old mice. Our previous study suggested Rwdd1 is a thymus-aging related protein (1).…”

Section: Discussionmentioning

confidence: 99%

“…To investigate the gene expression patterns in thymuses from aged mice, we performed differential display reverse (1) transcriptase polymerase chain reaction (DDRT-PCR) using total RNA from thymus of 1-month and 10-month old mice. Our previous study suggested Rwdd1 is a thymus-aging related protein (1). Currently, the detailed way of Rwdd1 functioning is still unclear.…”

Section: Discussionmentioning

confidence: 99%

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Rwdd1, a Thymus Aging Related Molecule, Is a New Member of the Intrinsically Unstructured Protein Family

et al. 2008

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We had previously identified a novel protein termed Rwdd1 whose expression in thymus is decreased in aged or oxidatively stressed mice. In the present study, we found that Rwdd1 expressed in both prokaryotic and eukaryotic cells showed a slower migration rate on SDS-PAGE gel. In addition, Rwdd1 was more sensitive to proteinase proteolysis. Furthermore, being a highly acidic protein which contains an RWD domain, Rwdd1 shared a high level of sequence similarity with Gir2, a member of the intrinsically unstructured protein (IUP). These findings suggest that Rwdd1 is a novel member of the IUP family. Cellular & Molecular Immunology. 2008;5(5):333-339.

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Identification and Characterization of a Novel Thymus Aging Related Protein Rwdd1

Abstract: By using DDRT-PCR and EST segment ligation, a novel mouse thymus involution related gene

Cited by 9 publications

References 26 publications

Thymus and aging: morphological, radiological, and functional overview

Thymus and aging: morphological, radiological, and functional overview

Early-life nutrition influences thymic growth in male mice that may be related to the regulation of longevity

Rwdd1, a Thymus Aging Related Molecule, Is a New Member of the Intrinsically Unstructured Protein Family

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