Identification and characterization of a novel 34 kDa merozoite protein in Babesia orientalis

He, Li; Fan, Lizhe; Liu, Qin; Hu, Jinfang; Miao, Xiaoyan; Huang, Yuan; He, Pei; He, Junwei; Yu, Liang; Khan, Muhammad Kasib; Zhou, Yanqin; Shen, Bang; Jun, Zhao

doi:10.1016/j.vetpar.2015.06.031

Cited by 1 publication

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“…How does B. orientalis interact with the host, invade red blood cells, grow and proliferate? Several antigens, such as AMA1, RAP1, BoP34, etc., have been identified and presumed to play important roles in B. orientalis invasion of RBC (Yu et al, 2014; He et al, 2015a,b). There is no report about the receptors and/or interacting proteins for these antigens.…”

Section: Future Researchmentioning

confidence: 99%

A Historical Overview of Research on Babesia orientalis, a Protozoan Parasite Infecting Water Buffalo

Liu

Yao

et al. 2017

Front. Microbiol.

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Babesiosis is a globally important zoonotic disease caused by tick-borne intraerythrocytic protozoan of the genus Babesia (phylum apicomplexa). In China, there are five species that infect cattle buffalo and cause great economic loss, which include Babesia bigemina, B. bovis, B. major, B. ovata, and B. orientalis. Among them, B. orientalis is the most recently identified new Babesia species epidemic in China. This review summarized the work done in the past 33 years to give an overview of what learned about this parasite. This parasitic protozoan was found in 1984 in Central and South China and then named as B. orientalis in 1997 based on its differences in transmitting host, morphology, pathogenicity and characteristics of in vitro cultivation when compared with B. bigemina and B. bovis. It was found that Rhipicephalus haemaphysaloides is the transmitting vector and water buffalo is the only reported host. Phylogenetic analysis based on the 18S rRNA gene also confirmed that B. orientalis is a new species. After species verification, four diagnostic methods including semi-nest PCR, loop-mediated isothermal amplification assay, reverse line blot hybridization assay, and real-time PCR were established for lab and field use purposes. Genomic sequencing was conducted and the complete genomes of mitochondria and apicoplast were annotated. Future work will be focused on developing effective vaccines, identifying drug targets and screening useful drugs for controlling B. orientalis in water buffalo.

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Section: Future Researchmentioning

confidence: 99%