Identification and Characterization of a <i>nodH</i> Ortholog from the Alfalfa-Nodulating Or191-Like Rhizobia

Papa, María Florencia Del; Pistorio, Mariano; Draghi, Walter Omar; Lozano, Mauricio Javier; Giusti, M. A.; Medina, Carlos; Dillewijn, Pieter van; Martı́nez-Abarca, Francisco; Flores, Beth; Ruíz-Sainz, José E.; Megı́as, Manuel; Pühler, Alfred; Niehaus, Karsten; Toro, Nicolás; Lagares, Antonio

doi:10.1094/mpmi-20-2-0138

Cited by 18 publications

(18 citation statements)

References 49 publications

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“…LPU83 strain is acid tolerant and has an extended nodulation host range (Del Papa et al, 1999). The genetic structure of this strain is unique in that it has a chromosome related to the bean-, pea-, and clover-nodulating rhizobia, while the pSym genes participating in nodulation and nitrogen fixation are more similar to those present in Medicago symbionts (Laguerre et al, 2001;Del Papa et al, 2007;Torres Tejerizo et al, 2011a). Previously, we described the conjugative properties of plasmids from Rhizobium sp.…”

Section: Introductionmentioning

confidence: 99%

Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background

Tejerizo

Pistorio

Althabegoiti

et al. 2014

FEMS Microbiol Ecol

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Plasmids have played a major role in bacterial evolution, mainly by their capacity to perform horizontal gene transfer (HGT). Their conjugative transfer (CT) properties are usually described in terms of the plasmid itself. In this work, we analyzed structural and functional aspects of the CT of pLPU83a, an accessory replicon from Rhizobium sp. LPU83, able to transfer from its parental strain, from Ensifer meliloti, or from Rhizobium etli. pLPU83a contains a complete set of transfer genes, featuring a particular organization, shared with only two other rhizobial plasmids. These plasmids contain a TraR quorum-sensing (QS) transcriptional regulator, but lack an acyl-homoserine lactone (AHL) synthase gene. We also determined that the ability of pLPU83a to transfer from R. etli CFN42 genomic background was mainly achieved through mobilization, employing the machinery of the endogenous plasmid pRetCFN42a, falling under control of the QS regulators from pRetCFN42a. In contrast, from its native or from the E. meliloti background, pLPU83a utilized its own machinery for conjugation, requiring the plasmid-encoded traR. Activation of TraR seemed to be AHL independent. The results obtained indicate that the CT phenotype of a plasmid is dictated not only by the genes it carries, but by their interaction with its genomic context.

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Section: Introductionmentioning

confidence: 99%

Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background

Tejerizo

Pistorio

Althabegoiti

et al. 2014

FEMS Microbiol Ecol

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“…Researches have indicated that at least two sets of sulfate activation systems exist in Rhizobium: one activates sulfate for the synthesis of cysteine, and APS directly generates sulfite catalyzed by APS reductase (cysH) (Sekowska et al 2000); the other is that sulfate is catalyzed to generate PAPS by ATP sulfurylase and APS kinase complex encoded by nodPQ gene, then PAPS is used to modify Nod factors and extracellular polysaccharides via sulfotransferase encoded by nodH gene (Ehrhardt et al 1995;Del Papa et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a gene associated with sulfate assimilation in Sinorhizobium fredii WGF03

Song

Shen

et al. 2014

World J Microbiol Biotechnol

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Sulfur is an essential element for rhizobia, such as sulfated modified Nod factors and nitrogenase. To investigate the role of sulfur metabolism in Rhizobium-Soybean symbiosis, a transponson random insertional mutants' library was constructed and a sulfur assimilation-related gene was isolated and characterized. A mutant strain unable to utilized sulfate was screened from 11,000 random insertional mutants of Sinorhizobium fredii WGF03. Sequencing analysis showed that a sulfate assimilation-related gene (cysDN) was inserted by the Tn transponson. Mutants inactivated in cysD and cysN (SMcysDF and SMcysNF) were constructed by homologous recombination using the suicide plasmid pK18mob. The mutants SMcysDF and SMcysNF could no longer utilize sulfate as sulfur source. Phenotype analysis revealed that mutation of cysDN had multiple effects on S. fredii WGF03. Root hair deformation assay showed that the activity of Nod factors secreted by mutants SMcysDR and SMcysNR elicited minimal hair initiation only. Soybean plant tests indicated that the mutant strains delayed 1-2 days to nodulate and exhibited lower nodulation efficiency and symbiotic efficiency than the wild-type strain. The complementary strain of cysD and cysN (HcysDF and HcysNF) could restore the nodulation efficiency.

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“…Lamentablemente, la estructura de los NFs de dicho mutante nodH no fue determinada, dejando abierto el interrogante de si podía existir otra enzima que produzca la sulfatación de los NFs o si esta cepa es capaz de nodular alfalfa sin poseer factores de nodulación sulfatados (Del Papa, et al, 2007).…”

Section: I6c Propiedades Simbióticas De Los Rizobios Tipo Oregonunclassified

“…Estos resultados, sin embargo, no fueron decisivos (Eardly, et al, 1992). Con la llegada de métodos filogenéticos más poderosos, Laguerre et al (2001) Papa et al (2007) estudiaron el gen nodH y encontraron relaciones filogenéticas similares a las descriptas previamente con respecto a los genes simbióticos (Laguerre, et al, 2001).…”

Section: I6dunclassified

“…Leucena leucocephala, altamente competitivos con E. meliloti en acidez e ineficientes en la fijación biológica de nitrógeno. Además, la constitución genética de los mismos ha impuesto severas restricciones a la taxonomía clásica de estos rizobios (Eardly, et al, 1985;Eardly, et al, 1992;Eardly, et al, 1995;Del Papa, et al, 1999;Wegener, et al, 2001;Del Papa, et al, 2007). )), Rhizobium etli CFN42 (CP000133 (González, et al, 2006) (AM236080 (Young, et al, 2006)) y Agrobacterium radiobacter K84 (CP000628 (Slater, et al, 2009) (Kurtz et al, 2004).…”

Section: Ii8d Secuencias Utilizadasunclassified

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Estudios genómicos y caracterización funcional de rizobios tipo Oregon noduladores de alfalfa y otras leguminosas

Tejerizo¹,

Gonzalo²

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Debido a sus características particulares fenotípicas y taxonómicas, los rizobios tipo Oregon han atraído la atención de los rizobiólogos desde su aislamiento original por Eardly et al. (1985) en Oregon, USA. Estos rizobios son tolerantes a la acidez, tienen un rango de hospedadores amplio (nodulan varias leguminosas), son muy competitivos para la nodulación de alfalfa en suelos ácidos y son ineficientes para la fijación de nitrógeno en asociación con la alfalfa. Todos estos factores señalan a este grupo de rizobios como un potencial factor de riesgo en los suelos agrícolas en los que coexisten y compiten con E. meliloti, el simbionte eficiente de alfalfa. Nueva información sobre la genética y fisiología de las bacterias tipo Oregon es necesaria para desarrollar mejores estrategias y herramientas (moleculares) para su detección, para comprender de modo más acabado su ecología en suelos de cultivo, y para minimizar los impactos sobre la simbiosis eficiente entre alfalfa y E. meliloti. Objetivos Generales: Abordar estudios genómicos y caracterizar funcionalmente a los rizobios tipo Oregon, noduladores de alfalfa y otras leguminosas, tolerantes a la acidez e ineficientes en la fijación biológica de nitrógeno. Objetivos Específicos: - Obtener el genoma completo de la cepa Rhizobium sp. LPU83, como el representante mejor caracterizado de los rizobios tipo Oregon. - Realizar el análisis funcional in silico del nuevo genoma y evaluar, comparativamente, marcadores cromosomales y plasmídicos respecto de sus homólogos en otros rizobios con el fin de establecer relaciones funcionales y filogenéticas con otras rizobacterias. - Analizar la información contenida en plásmidos, y su transmisibilidad por vía conjugativa. - Determinar la estructura de los factores de nodulación de las rizobios tipo Oregon como determinantes de especificidad para la nodulación de alfalfa. Explorar las relaciones estructurales de los factores de nodulación con los de otros rizobios, especialmente los noduladores de Medicago spp. y Phaseolus vulgaris.

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Identification and Characterization of a nodH Ortholog from the Alfalfa-Nodulating Or191-Like Rhizobia

Cited by 18 publications

References 49 publications

Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background

Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background

Isolation and characterization of a gene associated with sulfate assimilation in Sinorhizobium fredii WGF03

Estudios genómicos y caracterización funcional de rizobios tipo Oregon noduladores de alfalfa y otras leguminosas

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