Identification and characterization of a novel abiotic stress responsive sulphotransferase gene (OsSOT9) from rice

Abstract

Abiotic stresses may seriously affect plant growth and development. In order to explore new stress tolerance genes in rice (Oryza sativa L.), expression profiles were obtained for leaf and panicle tissues. They were taken at seedling, booting, heading and flowering stages of indica cultivar Pei'ai 64S plants under cold, drought and heat stresses by using the GeneChip Rice Genome Array (Affymetrix), which includes 51,279 transcripts from japonica and indica rice. O. sativa L. cytosolic sulphotransferase-like gene (OsSOT9) was highly induced in leafs and panicles during different developmental stages, in response to all stresses, especially at booting stage under cold stress. The quantitative real-time polymerase chain reaction analysis showed that this result was almost consensus with GeneChip Rice Genome Array. cDNA of OsSOT9 was cloned to study its function in stress tolerance through reverse transcription PCR. Sequence analysis showed that the cDNA encoded a protein with molecular weight of about 37.78 kDa and pl of about 6.6, which was composed of 343 amino acid residues. Bioinformatics data showed that the protein contained a conservative domain of the sulphotransferase (SOT) family gene. OsSOT9 was found to be closely related to the cytosolic sulphotransferase after comparison of the protein sequences. Analysis of the putative promoter region found 12 kinds of cis-elements related to stress response. On the basis of the above analyses, we suggested that OsSOT9 is a novel candidate gene involved in stress tolerance in rice.

Ocimum metabolomics in response to abiotic stresses: Cold, flood, drought and salinity

“…Na+/H+ (AtNHX1) and K+/H+ (AtNHX3) antiporters from Arabidopsis have been demonstrated for improved tolerance (CDS_39398_Unigene_61545) against salt and drought stresses in transgenic poplar [42]. Cytosolic sulfotransferase (OsSOT9) gene from rice has been reported as a novel candidate (CDS_38924_Unigene_60033) concerned to stress tolerance [43]. Wang et al [44] have demonstrated that RPN1a, the Arabidopsis 26S proteasome subunit (CDS_12189_Unigene_22188) is necessary for optimal growth of plant as well as stress responses.…”

Identification and characterization of a novel abiotic stress responsive OsTHIC gene from rice

“…According to the method described in previous studies [31,32], we obtained the experimental materials as follows. Seeds of cultivated Indica rice Pei'ai 64S were sterilized in 0.1% HgCl 2 for 10 min and washed three times under double-distilled water, immersed for 3 days under 25 C with water changed every day; then they were selected and germinated in liquid culture medium at 37 C for 2-3 days.…”

OsPM1 is a positive regulator of rice tolerance to drought stress but a negative regulator of rice tolerance to salt stress

“…Therefore, it is necessary to vigorously excavate some genes related to abiotic stress in rice, and use genetic engineering to study and modify them, so that rice has corresponding resistance under different stresses (Filiz and Akbudak 2020). This is of great significance for ensuring the normal growth and development of rice in harsh environments and maintaining stable and considerable yields (Cao et al 2016).…”